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Retno Sri Wahjuni
Department of Health, Faculty of Vocational,Universitas Airlangga
Arts Humanities Economics, Econometrics & Finance Education Public Health Social Sciences

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The Isolation DNA Chromosomal DNA from Local Isolate of Aeromonas hydrophila Bacteria from East Java Retno Sri Wahjuni; M. Gandul Atik Yuliani
Jurnal Vokasi Indonesia Vol 6, No 1 (2018): January - June
Publisher : Program Pendidikan Vokasi Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (185.995 KB) | DOI: 10.7454/jvi.v6i1.115

Abstract

Aeromoniasis is a bacterial disease caused by Aeromonas hydrophila that affects fish and shrimps in ponds and aquariums. This is an ulcer disease that causes petechiae in scales of fish and may be fatal. It can cause economic loss if it is not treated with medication accompanied with improved sanitation. It affects numerous freshwater fish farms in East Java. A previous study has characterized an antigenic protein derived from the outer membrane protein of A. hydrophila. In order to perform sequencing of the DNA coding for this protein, we first conducted a study for isolating DNA fragments of A. hydrophila via four stages: cultivation and harvesting of bacterial cells, cell lysis, DNA purification, and the concentration of chromosomal DNA. The results will be used as a predictive immunogenic determinant by the method by Kolaskar and Tongaonkar. Keywords: Aeromonas hydrophila, chromosomal DNA, immunogenic determinant
Co-Authors Gandul Atik Yuliani