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All Journal Jurnal Biologi Tropis Jurnal Pepadu
I Gde Mertha
Program Studi Pendidikan Biologi, FKIP Universitas Mataram
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Centella asiatica Activities towards Staphylococcus aureus and Escherichia coli Growth Sandi Murdiyansah; Dewa Ayu Citra Rasmi; I Gde Mertha
Jurnal Biologi Tropis Vol. 20 No. 3 (2020): September - Desember
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v20i3.1418

Abstract

Centella asiatica contains some secondary metabolic compounds, e.g. tannin, alkaloid, flavonoid, saponin, and triterpenoid, known as medicinal plants as well, one of which is as a anti-bacterial. The present study aimed at examining the activities of Centella asiatica extract towards the growth of Staphylococcus aureus and Escherichia coli. The extraction of C. asiatica was done by using ethanol and ethyl acetate solvents and the activity test employed seaweed diffusion. The hindrance power of Centella asiatica extract was tested in 30%, 50%, and 95% and the data were analyzed qualitatively with the standard issued by the Clinical and Laboratory Standard Institute and quantitatively with ANOVA. It shows that Centella asiatica extract in the ethyl acetate can hinder the growth of Staphylococcus aureus in the acetate concentration of 50% and 70% at the sensitive level and Escherichia coli at the intermediate level in the extract concentration of 50% and 70%. However, Centella asiatica activities in the ethanol solvent hinders the growth of either Centella asiatica or Escherichia coli with resistant category in the extract concentration of 70%. The hindrance power of Centella asiatica extract in ethyl acetate in the growth of Staphylococcus aureus is significantly more effective than that of Centella asiatica extract in the ethanol solvent
Response of Sweet Corn (Zea mays L. Saccharata) Growth on the Treatment of Organic and NPK Fertilizer Ahmad Raksun; I Wayan Merta; I Gde Mertha; Mohammad Liwa Ilhamdi
Jurnal Biologi Tropis Vol. 21 No. 1 (2021): Januari - April
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v21i1.2443

Abstract

Sweet corn fruit is one of the food favored by the people of Indonesia. The growth and yield of sweet corn can be increased by fertilizing. Research on the response of sweet corn growth to organic and NPK fertilizer was conducted in the Terong Tawah Village, West Lombok Regency. The purpose of this research was to determine: (1) response of the growth of sweet corn to the organic fertilizer treatment, (2) response of the growth of sweet corn to the NPK fertilizer treatment, (3) the effect of the interaction between organic and NPK fertilizer on the growth of sweet corn. The growth parameters of sweet corn are stem height, leaf lenght, number of leaf and stem diameter. Research data were analyzed using analysis of variance. The results showed that: (1) sweet corn growth has a positive response to the organic fertilizer, organic fertilizer can increas stem height, leaf lenght, number of leaf and stem diameter, (2)  sweet corn growth has a positive response to the NPK fertilizer, NPK fertilizer can increase all  growth parameter, (3) the interaction between organic and NPK fertilizer has no significant effect on all growth parameters, (4) the optimum dose of organic fertilizer is 1.5 kg m-2 of agricultural land, (5) The treatment of 6 g NPK fertilizer was better than other treatments.
INDUKSI POLIPLOIDI PADA TANAMAN BAWANG PUTIH (Allium sativum L.) A Sukarso; I Gde Mertha; Syamsul Bahri; Ahmad Raksun; I Wayan Merta
Jurnal Pepadu Vol. 4 No. 2 (2023): Jurnal Pepadu
Publisher : LPPM UNIVERSITAS MATARAM

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Abstract

Kromosom poliploid merupakan sumber belajar yang baik untuk menjelaskan mutasi kromosom pada materi genetika. Sel yang mempunyai lebih dari dua set kromosom dinamakan poliploid. Individu dengan sel poliploid dapat terjadi secara alami atau dibuat oleh manusia karena perlakuan zat kimia. Pengabdian ini diikuti oleh guru-guru mitra yang tergabung dalam MGMP Biologi Kabupaten Lombok Barat dan telah dilaksanakan di SMAN 1 Narmada. Tujuan kegiatan pengabdian adalah memberikan pengetahuan dan keterampilan pada guru-guru mitra tentang teknik induksi poliploidi sel-sel ujung akar bawang putih, preparasi preparat squash ujung akar untuk penghitungan jumlah kromosom dibawah mikroskop, dan penyusunan karyotipe. Metode untuk pencapaian tujuan pelatihan ini adalah praktik, ceramah, diskusi, dan tanya jawab. Aktivitas praktikum yang dilakukan guru mitra dengan pendampingan dari tim pengabdian adalah (1) Teknik perlakuan pelipatgandaan set kromosom dasar sel-sel ujung akar bawang putih (Allium sativum L.) dengan kolkisin, (2) Pembuatan preparat squash sel-sel mutan ujung akar bawang putih, dan (3) Pembuatan karyotipe dan penentuan ploidi sel-sel mutan berdasarkan hasil foto dibawah mikroskop yang telah disusun dalam karyogram. Hasil pelatihan menunjukkan (1) Guru mitra mendapat pembelajaran yang bermakna tentang teknik induksi poliploidi, teknik pembuatan preparat squash sel-sel poliploid ujung akar, dan teknik penyusunan karyotipe untuk membandingkan set kromosom diploid dan poliploid, (2) Berkat kerja tekun dan antusias yang tinggi, guru-guru mitra dapat menghasilkan produk preparat squash sel-sel ujung akar yang baik untuk pengamatan kromosom poliploid, dan (3) Pengetahuan dan keterampilan yang diperoleh guru mitra dapat menjadi bekal berharga untuk membuat preparat perubahan jumlah kromosom akibat mutasi pada kegiatan praktikum siswa di sekolah dalam menunjang materi genetika.
Co-Authors A Sukarso Ahmad Raksun Dewa Ayu Citra Rasmi I Wayan Merta Mohammad Liwa Ilhamdi Sandi Murdiyansah Syamsul Bahri