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All Journal Tropical Animal Science Journal
H. Maheshwari
Department of Anatomy, Physiology, and Pharmacology, Faculty of Veterinary Medicine, IPB University
Agriculture, Biological Sciences & Forestry Energy

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Physiological Repairing of Postpartum Rat Uterus Treated with Pelawan (Tristaniopsis obovata Benn.) Extract Yusfiati; W. Manalu; H. Maheshwari; Andryanto
Tropical Animal Science Journal Vol. 44 No. 4 (2021): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2021.44.4.415

Abstract

The study examined the effectiveness of ethyl-acetate fraction of Pelawan extract on the activities of superoxide dismutases in physiological repairing of uterus in postpartum rat based on SOD activity, MDA concentration, and Cu, Zn SOD expressions. The experiment was arranged with a factorial, completely randomized design. Seventy-two female rats were divided into 4 groups, namely, control 1 (P01= 9), control 2 (P02= 9), giving birth once (P1= 27), and giving birth twice (P2= 27). The experimental rats were given treatments orally with 4 doses, namely 0 mg/kg BW, 50 mg/kg BW, 100 mg/kg BW, and 150 mg/kg BW. Observation data were taken on days 0, 3, and 5 after parturition. Variables measured were SOD activity, MDA levels, and the number of cells in the uterine tissue expressing Cu, Zn SOD. The results of studies on the condition of P01, P02, P1, and P2 showed significant changes in Cu, Zn SOD expressions. The low of SOD and MDA activities were found at doses of 100 mg and 150 mg. The high number of cells expressing Cu, Zn SOD in the epithelium cells and gland cells were found at a dose of 100 mg. Antioxidant plant extracts and their dosages determine the activities of SOD, MDA, and Cu, Zn SOD expressions in the uterus that will affect the process of repairing and involution of uterine tissue. Allegedly, the bioactive compounds extracted at certain doses effectively affect the physiological repair of cells in the uterine tissue of postpartum mothers.
The Viabilities of Freeze-Thaw Pasundan-Bull Sperms After a Short-Term Exposure to Media with Different pHs R. Widyastuti; N. M. D. Haq; D. N. Pristihadi; Wahyudin; H. Maheshwari; C. Sumantri; A. Boediono
Tropical Animal Science Journal Vol. 45 No. 3 (2022): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2022.45.3.270

Abstract

External pH is crucial in preserving sperm viability and ensuring fertilization during in vitro conditions. The purpose of this study was to determine the maximum pH value that can be tolerated by frozen-thawed Pasundan bull sperms and the effect on sperm quality. Around 250x106 sperms/mL of frozen-thawed Pasundan bull sperms were divided into ten equal aliquots, and each was diluted in the medium within a particular pH value. HCL or NaOH was added to the buffer media to create ten different solutions with varying pH values of 3, 4, 5, 6 as acidic, 7.2-7.4 as a control, and 8, 9, 10, 11, and 12 as alkaline. Furthermore, the samples were incubated for 5 minutes at room temperature within a particular pH medium before being immediately supplemented with a buffered medium to achieve a pH of 7.2-7.4. After 10 minutes of incubation at room temperature, all parameters were assessed. The results showed that sperm motility, viability, normal morphology, and acrosome intactness in sperms incubated in the acidic or alkaline media were significantly lower compared to control (p<0.05, respectively). Interestingly, the sperm still had a good tolerance to pHs 6 and 8. This tolerance was evidenced by all the parameters of sperms that were not sharply decreased compared to the control group. The significant loss of motility occurred at pHs 3 and 12. It could be concluded that frozen-thawed Pasundan bull sperms are still tolerable in pHs 4-11, but the sperm quality degrades as the acidity or alkaline level increases.acidic
Co-Authors A. Boediono Andryanto C. Sumantri Diah Nugrahani Pristihadi N. M. D. Haq R. Widyastuti W. Manalu Wahyudin Yusfiati Yusfiati