Article Per Year (5 Year)
p-Index From 2019 - 2024
0
P-Index
This Author published in this journals
All Journal Indonesian Journal of Chemistry
Agung Endro Nugroho
Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta 55281
Chemical Engineering, Chemistry & Bioengineering Chemistry

Published : 1 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 1 Documents
Search
Journal : Indonesian Journal of Chemistry

 1 
Quantification of Andrographolide Isolated from Andrographis paniculata Nees Obtained from Traditional Market in Yogyakarta Using Validated HPLC Yandi Syukri; Ronny Martien; Endang Lukitaningsih; Agung Endro Nugroho
Indonesian Journal of Chemistry Vol 16, No 2 (2016)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (276.901 KB) | DOI: 10.22146/ijc.21163

Abstract

This research was aimed to quantification of andrographolide isolated from A. paniculata Ness found in traditional market in Yogyakarta using validated HPLC to obtain high level content of andrographolide. The extraction of andrographolide from A. paniculata was carried out using ethanol as the solvent. Fractionation and isolation were continued using a non-polar solvent. Next, the extracts were re-crystallized to obtain isolated andrographolide. The identity of the compound was confirmed through an analysis of the melting point, IR spectra, and TLC. The purity of the compound was confirmed by the validated HPLC. The data obtained were then compared using an analytical grade of andrographolide as the standard. The isolated andrographolide confirmed melting point, IR spectra and TLC analysis were similar to the standard andrographolide. The method to determine the content of isolated andrographolide showed an adequate precision, with a relative standard deviation (RSD) smaller than 1%. The accuracy showed good recovery values were obtained for all concentrations used. The HPLC method in this study showed specificity and selectivity with linearity in the working range and good precision and accuracy, making it very suitable for the quantification of andrographolide isolated in A. paniculata. When compared to the standard, the purity of the isolated andrographolide was 95.74 ± 0.29%.
Co-Authors Endang Lukitaningsih Ronny Martien Yandi Syukri