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All Journal Journal of Mathematical and Fundamental Sciences Acta Pharmaceutica Indonesia Indonesian Journal of Chemistry Current Research on Biosciences and Biotechnology
Indra Wibowo
Physiology Animal Development and Biomedical Science, School of Life Science and Technology, Institut Teknologi Bandung, Jl. Ganesha No. 10, Bandung 40132, West Java, Indonesia
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics

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SKRINING TOKSISITAS AKUT LIMA RIMPANG SUKU ZINGIBERACEAE MENGGUNAKAN EMBRIO IKAN ZEBRA Anggra Paramita; Indra Wibowo; Muhamad Insanu
Acta Pharmaceutica Indonesia Vol. 46 No. 2 (2021)
Publisher : School of Pharmacy Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/api.v46i2.16093

Abstract

Tumbuhan suku Zingiberaceae dikenal sebagai sumber obat tradisional. Pemanfaatan sebagai tanaman obat sudah dikenal secara turun temurun dari generasi ke generasi. Oleh sebab itu, perlu dilakukan pengujian toksisitas untuk mengevaluasi dan memprediksi keamanan penggunaan tanaman obat tersebut. Tujuan dari penelitian ini adalah untuk mengetahui toksisitas akut dari ekstrak dan fraksi temu hitam (Curcuma aeruginosa Roxb.), temu mangga (Curcuma mangga Roxb.), jahe merah (Zingiber officinale Rosc. var. rubrum), bangle (Zingiber cassumunar Roxb.) dan kencur (Kaempferia galanga L.) berdasarkan nilai LC50 (Lethal concentration 50). Proses ekstraksi rimpang dari tanaman temu hitam, temu mangga, jahe merah, bangle, dan kencur dengan ekstraksi sinambung menggunakan pelarut etanol 70%. Ekstrak yang dihasilkan difraksinasi menggunakan pelarut bertingkat (n-heksan, etil asetat dan air). Pengujian toksisitas akut dilakukan menggunakan ikan zebra. Dari pengamatan diperoleh perbedaan morfologi embrio dan larva ikan zebra yang dipaparkan dengan ekstrak etanol, fraksi etil asetat maupun fraksi air jika dibandingkan dengan kontrol negatif yang berisi media E3 1X. Perbedaan tersebut antara lain edema perikardial, edema kantong kuning telur (yolk sac), tulang belakang lengkung, tulang ekor lengkung, koagulasi dan malformasi rahang. Hasil nilai LC50  diperoleh melalui perhitungan regresi probit  kemudian data diekstrapolasi ke dalam golongan sesuai dengan kategori toksisitasnya. Dari penelitian diperoleh hasil bahwa ekstrak, fraksi etil asetat maupun fraksi air dari sampel tergolong dalam toksisitas sedang, sedikit beracun, dan praktis tidak beracun.  
Fibroin and Spidroin Thin Film to Support the Attachment and Spread of Human Dermal Fibroblast: The Potency of Skin Tissue Engineering Safira Meidina Nursatya; Anggraini Barlian; Hermawan Judawisastra; Indra Wibowo; Hutomo Tanoto
Journal of Mathematical and Fundamental Sciences Vol. 53 No. 2 (2021)
Publisher : Institute for Research and Community Services (LPPM) ITB

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/j.math.fund.sci.2021.53.2.10

Abstract

This study aimed to determine the characteristics of scaffolds made of fibroin from Bombyx mori and spidroin from Argiope appensa in supporting the attachment and proliferation of HDF cells on the scaffolds. Thin-film scaffolds were made using the solvent casting technique, where the scaffold is an amalgamation of fibroin, spidroin, PVA, and glycerol. HDF cells were grown on DMEM medium with 10% FBS and 1% antibiotic-antimicotic. Characterization of the scaffolds was performed by using ATR-FTIR, swelling test, contact angle measurement, tensile test, biodegradation, MTT and SEM. The results of the ATR-FTIR analysis showed that the scaffolds contained fibroin, spidroin, PVA, and glycerol. Swelling and contact angle tests showed that all scaffold combinations were hydrophilic. Mechanical properties and in vitro biodegradation tests showed no significant difference among the scaffold combinations. MTT testing showed that all scaffolds could facilitate the attachment of fibroblasts and showed increased viability from day 1, 3, and 5. Scanning electron microscopy showed that the cells in the 70% fibroin and 10% spidroin scaffold had the best cell morphology and the best combination for potential application in skin tissue engineering.
A study of genus Zingiber: the role of condiments in science Anggra Paramita; Indra Wibowo; Muhammad Insanu
Current Research on Bioscences and Biotechnology Vol. 3 No. 1 (2021)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2021.3.1/CKHEYSRT

Abstract

Zingiberaceae family has been widely used as an herbal medicine from generation to generation. The literature review of Zingiber genus, which is part of Zingiberaceae, was conducted to provide information and to determine the correlation between bioactive compounds and pharmacological properties with their empirical uses. Articles about genus Zingiber were collected from online databases (e.g., Directory of Open Access Journal, PubMed, ResearchGate, ScienceDirect, and Springer), they were sorted based on inclusions criteria such as related to bioactive compounds and biological activities of the samples and also articles for the last ten years. Merely articles with DOI were reviewed. The screening process resulted in 52 reports being reviewed and grouped based on each plant's bioactive contents and pharmacological properties. The major constituents in genus Zingiber are essential oils. Therefore, the dominant activities found in this genus was related to antimicrobial and antioxidant. Furthermore, the other activities of plants were related to the bioactive compounds and their current uses. The empirical uses of these rhizomes were in line with the bioactive compounds and biological activities.
In Silico Study on Interaction and Preliminary Toxicity Prediction of Eleutherine americana Components as an Antifungal and Antitoxoplasmosis Candidate Sophi Damayanti; Nadiyah Athifah Salim Martak; Benny Permana; Adi Suwandi; Rika Hartati; Indra Wibowo
Indonesian Journal of Chemistry Vol 20, No 4 (2020)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (15.96 KB) | DOI: 10.22146/ijc.48570

Abstract

Red bulbs of Eleutherine americana (Aubl.) Merr. ex K. Heyne has been known for its high content of naphthoquinones that have antifungal and antiparasitic activities. In this research, in silico interaction study was performed between 31 compounds reported to be found in E. americana with the selected target proteins for antifungal and antitoxoplasmosis activity using the molecular docking method. An ORPs (OSBP-related proteins), Osh4 (PDB ID: 1ZHX), and N-myristoyltransferase (Nmt, PDB ID: 1IYL) were used as the antifungal target proteins. Toxoplasma gondii purine nucleoside phosphorylase (TgPNP, PDB ID: 3MB8) and calcium-dependent protein kinase-1 (TgCDPK1, PDB ID: 4M84) were used as antitoxoplasmosis target proteins. Three-dimensional structures of the test compounds were made and optimized using GaussView 6.0 and Gaussian 09W. The target proteins were prepared using the Discovery Studio 2016 Program. Aquatic toxicity prediction as the preliminary assessment of the safety of the compounds was performed using ECOSAR v2.0. The results suggest that the compound having both the smallest free binding energy compared with positive control and other test compounds and low predicted toxicity is β-sitosterol with a free binding energy of ‒11.55 and ‒11.18 kcal/mol towards Osh4 and Nmt and ‒8.06 and ‒10.29 kcal/mol towards TgPNP and TgCDPK1, respectively.
Co-Authors Adi Suwandi Anggra Paramita Anggra Paramita Anggraini Barlian Benny Permana Hermawan Judawisastra Hutomo Tanoto Muhamad Insanu Muhammad Insanu Nadiyah Athifah Salim Martak Rika Hartati Safira Meidina Nursatya Sophi Damayanti