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Atin Supiyani
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Uji Efektivitas Vaksin Flu Burung Subtipe H5N1 pada Ayam Kampung di Legok, Tangerang, Banten Arria Janovie; Rusdi Rusdi; Atin Supiyani
Bioma Vol 10 No 2 (2014): Bioma
Publisher : Biologi UNJ Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (131.292 KB) | DOI: 10.21009/Bioma10(2).6

Abstract

Abstract The aim of the study was to determine the effectiveness of Avian Flu Vaccine Subtype H5N1 on native chicken. There were several inclusion criteria to selected the chicks. The criteria were just native chicken in Legok, at least 8 weeks old and never been had previous vaccine program. Total of 20 chickens were used as samples. All chicken was split in two cages, cage A contained 10 chickens were treated with vaccine and cage B contained 10 chickens as a control. Then 0.5 mL of vaccine administered intramuscularly in the chicken breast. The effectiveness of vaccination determined by measuring antibody titers before and 3 weeks after vaccination program. Measurement of antibody titers obtained by HI (Hemagglutination Inhibition) test method. Result showed that antibody titers of native chicken increased after vaccination program. There were 70% of samples that have been vaccinated could be protected against the virus. Three chickens as a control had positive titers prior to vaccination with a range of 21 - 22. Key Word : test of effectiveness, homologue killed vaccine, antibody titers.
PRE TREATMENT MINYAK JELANTAH DENGAN KARBON AKTIF AMPAS TEBU MENURUNKAN RESIKO HEPATOTOKSISITAS TIKUS (SPRAQUE DAWLEY) Sri Rahayu; Atin Supiyani; Rizky Darmansyah; Rizka Amalia
Bioma Vol 18 No 2 (2022): Bioma
Publisher : Biologi UNJ Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21009/Bioma18(2).5

Abstract

Cooking oil is a human need that is increasing over time resulting in wasted cooking oil. One of the efforts to reduce negative effect of used cooking oil is the use of activated carbon in the refinery process. The aim of this study was to determine the hepatotoxicity of used cooking oil treated with activated carbon of bagasse on rat livers. The method used true experimental laboratory with Post Test Only with Control Group Design. White rats were divided into 4 treatment groups. Positive control, given 0.2 ml of used cooking oil. Negative control, given 0.2 ml of packaged cooking oil. Group 3 was given 0.2 ml of used cooking oil mixed with 5% activated carbon. Group 4 was given 0.2 ml of used cooking oil mixed with 15% activated carbon of bagasse. The liver enzyme of SGPT-SGOT was measured by spectrophotometry and analysed by ANOVA. The concentration of bagasse-activated carbon in used cooking oil affected the levels of SGOT-SGPT and rats weight. The lowest levels of SGOT were obtained in used cooking oil treated with 5% activated carbon bagasse (18.85 ± 0.544 U/L). Application of bagasse-activated carbon in used cooking oil can reduce the risk of hepatotoxicity in mice.