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Alim Isnansetyo
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MONITORING PARASIT PADA BUDIDAYA IKAN DAN UDANG DI DAERAH ISTIMEWA YOGYAKARTA Triyanto Triyanto; Alim Isnansetyo
Jurnal Perikanan Universitas Gadjah Mada Vol 6, No 1 (2004)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.9041

Abstract

Parasites often cause the great loss in aquaculture either in fish or shrimp aquacultures. The loss will become more serious when fish or shrimp are secondary infected especially by bacteria. The objective of this study was to identify parasites in fish and shrimp culture inYogyakartaSpecialProvince (DIY). Samples of fish and shrimp were collected from several aquaculture centers in this region including several fish and shrimp hatcheries. Observation of parasites was conducted microscopically and macroscopically.Results showed that giant tiger prawn (Penaeus monodon) cultured in DIY was often infected by Zoothamnium sp. and Gregarines sp.. In addition, giant freshwater prawn (Machrobrachium rosenbergii) was often infected by Apiosoma sp., Dactylogyrus sp., Epystilis sp., Glossatella piscicola, Gregarines sp. and Zoothamnium sp.. Moreover Apiosoma sp.,  Dactylogyrus sp., Gyrodactylus sp., Ichthyopthirius sp., and Trichodina sp. were found in nile (Oreochromis niloticus) culture. Similar parasites such as Apiosoma sp., Dactylogyrus sp., Henneguya sp. Ichtyoptirius sp. and Trichodina sp..were found in Osphronemus gaurami. Ichtyoptirius sp. and Trichodina sp. also were found in walking catfish (Clarias gariephinus). 
ISOLASI, IDENTIFIKASI DAN KARAKTERISASI Vibrio spp. PATOGEN PENYEBAB VIBRIOSIS PADA KERAPU DI BALAI BUDIDAYA AIR PAYAU SITUBONDO Kamiso Handoyo Nitimulyo; Alim Isnansetyo; Triyanto Triyanto; Indah Istiqomah; Muhammmad Murdjani
Jurnal Perikanan Universitas Gadjah Mada Vol 7, No 1 (2005)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.9053

Abstract

This research was conducted to isolate, identify, and characterize pathogenic Vibrio spp., causative agents of vibriosis in grouper at Brackishwater Aquaculture Development Center (BADC), Situbondo. Twenty-nine isolates were isolated from wound, gills, eyes, liver, and ren of grouper with vibriosis on Thiosulphate Citrate Bile Sucrose Agar (TCBSA) medium. Koch Postulate Test was conducted to determine pathogenic Vibrio spp., by intraperitoneal injection to humpback grouper (Cromileptes altivelis) (9-10 cm of total length) at 106 cells/fish in triplicates. Results indicated that the pathogenic Vibrio spp., causative agents of vibriosis in grouper at BADC, Situbondo were identified to be V. alginolyticus, V. anguillarum, V. metchnikovii, V. vulnificus, V. fluvialis, V. furnisii, and V. parahaemolyticus. Infection of the pathogenic Vibrio spp. caused acute mortality within 17-46 hours with specific disease signs like haemorhagic on fins (pinnae pectorales, pinnae abdominales, pinna analis) and also on the body. These results suggested that vibriosis should be monitored and controlled properly and quickly.
EFEKTIVITAS VAKSIN POLIVALEN UNTUK PENGENDALIAN VIBRIOSIS PADA KERAPU TIKUS (Cromileptes altivelis) Kamiso Handoyo Nitimulyo; Alim Isnansetyo; Triyanto Triyanto; Muhammad Murdjani; Lili Sholichah
Jurnal Perikanan Universitas Gadjah Mada Vol 7, No 1 (2005)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.9056

Abstract

The objective of this research was to know the effectiveness of polyvalen  Vibrio vaccine to control vibriosis in humpback grouper (Cromileptes altivelis). The effectiveness of vaccine was evaluated by the survival rate (SR), relative percent survival (RPS), mean time to death (MTD) as well as growth rate of vaccinated fish. This research consisted of 4 treatments (control, injection, immersion, and oral vaccinations) in quadruplicates. Injection  vaccination was conducted by intraperitoneal injection of polyvalen vaccine at 107 cells/fish. Immersion vaccination was done by immersing the fishes at 107 cells/ml for 30 minutes. Oral administration of vaccine was also carried out  at 107 cells/fish. One week after the first vaccination, second vaccination (booster) was carried out at the same dosage and by the same administration. One week after the second vaccination, fishes were challenged with 3.16x104 cells/fish of Vibrio ordalii 3J by intraperitoneal injection, and reared for 20 days post infection. Results indicated that polyvalen Vibrio vaccine increased SR (P<0.01) up to 100%. Vaccination was also able to delay MTD of fishes. However, the vaccination was not influence the growth rate of fish.
Co-Authors INDAH ISTIQOMAH Kamiso Handoyo Nitimulyo Lili Sholichah Muhammad Murdjani Muhammmad Murdjani Triyanto Triyanto