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All Journal Jurnal Ilmiah Farmasi Akademi Farmasi Jember
Siti Nur Azizah
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Medicine & Pharmacology

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OPTIMASI HIDROLISIS MEDIA PRODUKSI CANGKANG UDANG MENGGUNAKAN KITINASE SERRATIA MARCESCENS KAHN.15.12 TERHADAP KADAR N-ASETIL GLUKOSAMIN SEBAGAI BAHAN SEDIAAN Siti Nur Azizah, Rosida
JURNAL ILMIAH FARMASI AKADEMI FARMASI JEMBER 2017: Desember - Vol.2 No.2
Publisher : AKADEMI FARMASI JEMBER

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (299.69 KB) | DOI: 10.53864/jifakfar.v2i2.28

Abstract

Limbah cangkang udang mengandung kitin yang tinggi yaitu sekitar 17-40%. Kitin tersebut dapat digunakan sebagai substat pertumbuhan bakteri kitinolitik yaitu S.marcescens KAHN 15.12. Bakteri tersebut akan mensekresikan enzim kitinase untuk mendegradasi kitin sebagai media pertumbuhannya hingga menghasilkan monomer N-asetil glukosamin (GlcNac). N-asetil glukosamin dapat dimanfaatkan sebagai bahan sediaan suplemen osteoartritis. Penelitian ini bertujuan untuk memproduksi GlcNac hasil hidrolisis kitin cangkang udang menggunakan kitinase Serratia marcescens KAHN.15.12. Hasil konfirmasi aktivitas kitinolitik menggunakan media selektif yaitu agar koloidal kitin 0,3% menunjukkan bahwa Serratia marcescens KAHN 15.12 memiliki index kitinolitik sebesar 2,3 yang ditunjukkan dengan adanya zona bening disekitar koloni. Hasil optimasi perbedaan konsentrasi media produksi menunjukkan bahwa tepung cangkang udang 2% menghasilkan kadar GlcNac tertinggi yaitu sebesar 376,35 μg/ml setelah diuji dengan metode kolorimetri menggunakan reagen shcales pada panjang gelombang 420nm. Berdasarkan hasil tersebut menunjukkan bahwa setiap hidrolisis 1 gram tepung cangkang udang yang mengandung 18,7% kitin akan menghasilkan sebesar 70,54 mg GlcNAc.Kata Kunci: N-asetil glukosamin, kitinase, Serratia marcescens
ANTIBACTERIAL ACTIVITY Streptomyces spp. ENDHOPHYTIC TO Staphylococcus aureus AND Eschericia coli Siti Nur Azizah
JURNAL ILMIAH FARMASI AKADEMI FARMASI JEMBER Vol 4, No 1 (2021): JURNAL ILMIAH FARMASI AKADEMI FARMASI JEMBER
Publisher : AKADEMI FARMASI JEMBER

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (455.406 KB) | DOI: 10.53864/jifakfar.v4i1.50

Abstract

The objective of this research was to known antibacterial activity of 4 endhophytic Streptomyces namely Streptomyces griseorubiginosus,   Streptomyces vellosus, Streptomyces diastaticus and Streptomyces griseoruber against Staphylococcus aureus and Eschericia coli and measure the biggest antibacterial activity which is indicate by clear zone diameter. Antibacterial activity by using agar plug diffusion method showed that Streptomyces vellosus has capable to inhibits growth of Staphylococcus aureus but not with Eschericia coli and Streptomyces diastaticus has capable to inhibits growth of Eschericia coli but not with Staphylococcus aureus. The clear zone of  Streptomyces vellosus and          Streptomyces diastaticus are 5,18 mm and 7 mm respectively.                Streptomyces griseoruber and Streptomyces griseorubiginosus can’t grow during isolation.  Keyword: Streptomyces vellosus, Streptomyces diastaticus, agar plug diffusion method
Identifikasi dan Uji Toksisitas Ekstrak Jamur Blotong dengan Metode Brine Shrimp Lethality Test (BST) Siti Nur Azizah, Lutfiya Cahyani, Reni Budiarti, Rudju Winarsa
JURNAL ILMIAH FARMASI AKADEMI FARMASI JEMBER 2016: Desember - Vol.1 No.2
Publisher : AKADEMI FARMASI JEMBER

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (329.091 KB) | DOI: 10.53864/jifakfar.v1i2.14

Abstract

Blotong mushroom is a fungus that thrives in waste of filter cake (a solid waste of sugar mill) from PG Semboro, Jember Regency.Commonly, blotong mushroom is often consumed by people but some were poisoned. The purpose of this study was to present a scientific data on blotong mushroom taxonomy and ensure the toxic content of blotong mushrooms based on the difference in age according to Brine Shrimp Lethality Test (BST) method. Identification carried to genus level by macroscopic and microscopic observations based on the book of Identify Mushrooms To Genus I and VI. While the toxicity test was conducted by BST following the extraction method using ethanol 99%, preparation test solutions, toxicity test of mushroom extract using the larvae of Artemia salina L and data analysis using a probit analysis then compared through variance T test with 95% confidence levelThe identification showed that blotong mushroom belonged to genus Leopita with specific features such us the cap had scales, the stem had a ring that appears in adulthood and the spore form of amyloid. The toxicity test of probit analysis showed that blotong mushroom is toxic on different ages such us mushroom with closed cap and opened cap had an average LC50 of 234.709 mg/ml and LC50 of 583.902 mg/ml, respecvtively, according to BST result. While T Test showed the significance value of 0.273 0.05, it means that there is no significant difference between the toxic content on mushrooms blotong with age difference.Keywords: identification, blotong mushroom, toxic, Brine Shrimp Lethality Test
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