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Rahmi Utami

Department of Obstetric and Gynecology, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia

Author-ID : 408790

Agriculture, Biological Sciences & Forestry Education Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Physics Social Sciences Other

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1

Influence of 17β-estradiol treatment on the expression of NF-κB in complete hydatidiform mole culture Nurseta, Tatit; Irwanto, Yahya; Utami, Rahmi
Medical Journal of Indonesia Vol 22, No 4 (2013): November
Publisher : Faculty of Medicine Universitas Indonesia

Background: Genetic evidence has established a role of nuclear factor kappa B (NF-κB) signaling in oncogenesis. However, activity of NF-κB in complete hydatidiform mole (CHM) cell culture under 17β-estradiol (E2) treatment is not yet known. Recently, a positive cross-talk between estrogen receptor (ER) and NF-κB to promote survival and progress of cancer cells to a more aggressive phenotype was established. In the present study, we examined the influence of E2 treatment on the NF-κB expression in CHM’s culture.Methods: This experimental study measured the expression of NF-κB in CHM culture treated with E2: 10, 100, 300, 600, and 1000 pg/mL and without E2. Imunohistochemistry staining was used to assess the expression of NF-κB. Microphotographs were taken using 400x magnification. Adobe photoshop CS2 was used to assess the NF-κB expression in cell nucleus. The lower the color intensity of cell RGBbv, is the higher the expression of NF-κB in cells. ANOVA test was performed to compare the expression of NF-κB.Results: NF-κB expression as indicated by color intensity in control group was 114.84 ± 9.02. NF-κB expression in E2 treatment groups were respectively: E2 10 pg/mL: 106.30 ± 13.95; E2 100 pg/mL: 82.47 ± 4.72; E2 300 pg/mL: 82.24 ± 2.67; E2 600 pg/mL: 69.05 ± 6.47; E2 1000 pg/mL: 68.49 ± 2.37. There was progressive decline in color intensity of cells with E2 treatment indicating the increase expression of NF-κB. Significant differences with the control group occurred in doses of E2 100, 300, 600, dan 1000 pg/mL.Conclusion: Treatment of CHM trophoblast culture with escalating doses of E2 was associated with the increase of NF-κB expression in a dose dependent manner. (Med J Indones. 2013;22:197-201. doi: 10.13181/mji.v22i4.599)Keywords: 17-β Estradiol, Hydatidiform mole, NF-κB

POLA PENDIDIKAN AQIDAH ANAK DALAM KELUARGA MUALAF (Studi Kasus di Mualaf Center Yogyakarta) Utami, Rahmi
G-Couns: Jurnal Bimbingan dan Konseling Vol 2, No 2 (2018): G-Couns Jurnal Bimbingan dan Konseling
Publisher : Universitas PGRI Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31316/g.couns.v2i2.65

Penelitian ini bertujuan untuk memperoleh proses pola pendidikan aqidah anak dalam keluarga mualaf studi kasus di Mualaf Center Yogyakarta, sehingga mampu memberikan gambaran kepada keluarga-keluarga lain dalam memberikan pendidikan aqidah anak di keluarga. Subjek penelitian ini berjumlah 2 keluarga dengan usia pernikahan 16 dan 13 tahun serta usia anak antara 6-15 tahun. Metode penelitian yang digunakan adalah pendekatan kualitatif. Metode penentuan subjek menggunakan teknik purposive sampling. Teknik pengumpulan data dengan pengamatan, wawancara dan dokumentasi. Hasil penelitian menunjukkan bahwa pemahamaan agama anak di keluarga mualaf bersifat: unreflective (tidak mendalam), egosentis (sesuai dengan keinginannya), imitative (meniru) dan verbal dan ritual. Terdapat dua pendekatan dalam menanamkan aqidah oleh keluarga mualaf, yaitu: 1) mendidik langsung (direct education), 2) mendidik tidak langsung (indirect education. Hambatan yang dialami oleh keluarga mualaf dalam pelaksanaan pendidikan aqidah, meliputi: 1) hambatan berdasarkan orangtua, 2) hambatan berdasarkan anak, 3) hambatan berdasarkan pola yang digunakan, dan 4) hambatan berdasarkan lingkungan.Kata kunci: pendidikan, aqidah, mualaf

Application of ultraviolet light as an indoor disinfectant Khair, Hafizhul; Suryati, Isra; Utami, Rahmi
ABDIMAS TALENTA: Jurnal Pengabdian Kepada Masyarakat Vol. 5 No. 2 (2020): ABDIMAS TALENTA : Jurnal Pengabdian Kepada Masyarakat
Publisher : Talenta Publisher

WHO announced a new outbreak disease, COVID-19, as a public health emergency that has plagued the world since January 30, 2020. Critical readiness and response are needed, such as equipping healthcare workers and facility management with important information, procedures, and tools to be safe and working effectively in responding to the outbreak. One of the critical hospital's services facilities is an isolation room/inpatient room. This room needs to be sterilized using a disinfectant regularly. The use of disinfectants, such as chemical fluids can only kill vegetative cells from microbes/viruses. Microbes/viruses, especially in the form of vegetative cells, could be killed by ultraviolet exposure. The objectives of this service program are: (1) Improving the cleanliness of hospital inpatient rooms so that they can be used immediately in treating patients (2) Speeding up the process of cleaning/sterilizing hospital inpatient rooms/isolation given the increasing number of patients in monitoring for covid-19 cases. The problem with inpatient sanitation/isolation in hospitals is usually many microbes in the indoor air. Clean and sterile inpatient/isolation rooms are needed in the patient's care. Therefore, it is necessary to improve the process of cleaning /sterilizing the inpatient room/isolation in the hospital by using UV exposure.

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