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Leila Nur Azizah
Fakultas Kedokteran Hewan, Institut Pertanian Bogor, Jl. Agatis, Kampus IPB Dramaga, Bogor, Indonesia
Veterinary

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Deteksi Gen Penyandi Resistansi Tetracycline dan Plasmid Mediated Quinolones pada Salmonella Ayam di Bandung dan Purwakarta Leila Nur Azizah; Agustin Indrawati; I Wayan Teguh Wibawan
Jurnal Veteriner Vol 23 No 1 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (644.554 KB) | DOI: 10.19087/jveteriner.2022.23.1.55

Abstract

This study aimed to identify genes encoding tetracycline and plasmid-mediated quinolones resistance to Salmonella spp from Poultry Farm in Bandung and Purwakarta, West Java. A total of 70 samples were collected from poultry farm In Bandung and Purwakarta, West Java. All isolates were test by selective media (SSA) and confirmation Salmonella with PCR. Thirty three isolate positive from selective media Salmonella Shigella Agar (SSA) and 21 isolat was confirmed as Salmonella spp by PCR. Twenty one isolate isolated were tested for tetracycline, doxycicline,, nalidixic acid, oxytetracycline, enrofloxacyn using disk diffusion method. TE-resistant were screened for presence of tet(A) and tet(B) genes by single polymerase chain reaction (PCR). The qnr(A), qnr(B) and qnr(S) genes were detected by multiplex PCR in quinolone resistant Salmonella isolates. The result of antibiotic sensitivity test showed that resistance to ampicillin (95.2%), tetracycline (100%), oxytetracycline (95.2%), nalidixic acid (90.4%), eritromisin (85.7%), enrofloxacin (76.2%), Gentamisin 47.6%, chloramphenicol (38.1%). The distribution of antibiotics-resistance genes in the Salmonella isolates included ampC (95.2%), tet(A)(61.9%), tet(B)(38.1%), qnr(A)(28.5%), qnr(B)(14.3%) and qnr(S)(23.8%).This study shows that a few pathogens of Salmonella are resistant to ampicillin, tetracycline , and quinolone. The tet and qnr genes are responsible for this resistance among Salmonella in Bandung and Purwakarta, West Java Indonesia was high.
Co-Authors Agustin Indrawati I wayan Teguh Wibawan