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All Journal ILMU KELAUTAN: Indonesian Journal of Marine Sciences Indonesian Aquaculture Journal
Andi Aliah Hidayani
Fisheries Department, Faculty of Marine Science and Fisheries, Hasanuddin University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences

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Journal : ILMU KELAUTAN: Indonesian Journal of Marine Sciences

 1 
Molecular Identification and Phylogenetic Trees Reconstruction of Blue Swimming Crabs (Decapoda: Portunidae) from Pangpang Bay, Banyuwangi Sapto Andriyono; Reza Istiqomatul Hidayah; Laksmi Sulmartiwi; Andi Aliah Hidayani; Md. Jobaidul Alam
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 27, No 2 (2022): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ik.ijms.27.2.93-100

Abstract

Crabs are a group of Decapoda (Portunidae) that act as keystone species from Pangpang Bay as the marine benthic organism. Besides having an ecological function, crab also provides essential components for human health. The crab identification technique is usually conducted based on morphology and anatomy characteristics, in which certain body parts as the key for identification. This study used two identification methods, i.e. morphological features and a molecular approach. Although morphological identification has been carried out, the molecular techniques provide better accuracy and, at the same time, provide additional information about the characteristics of mitochondrial DNA. The purpose of this study is to identify the blue swimming crab caught by a traditional fisherman at Pangpang Bay, Banyuwangi, based on mitochondrial DNA sequence on cytochrome c oxidase subunit I, and reconstructed the phylogenetic tree including genetic distance also was analysed. The nucleotide sequences of the COI gene were analysed by Chromas, Clustalω, Reverse-Complement, and the MegaX. The phylogenetic tree and genetic distance calculations were carried out using Mega X software through the Neighbor-Joining (NJ) Algorithm with the addition of several sequences from the NCBI online database. This study confirmed that the specimen of Pangpang Bay is Portunus pelagicus (BWIPP001 and BWIPP003) and Portunus sanguinolentus (BWIPP002). The species of P. pelagicus have 99.99% similarities with the same species (KJ168060) from China, while the P. sanguinolentus is close to the same species (EU284144) with a per cent identity is 99.97%. The genetic distance, for P. pelagicus and P. sanguinolentus, were in range of 0.00-0.066 and 0.00-0.005 respectively.
Molecular Identification of Snapper (Perciformes: Lutjanidae) Landed at Pondokdadap Fishing Port of Sendang Biru, Malang, Indonesia Sapto Andriyono; Novian Aji Pradana; Laksmi Sulmartiwi; Andi Aliah Hidayani; Md. Jobaidul Alam; Adrian Damora; Ahasan Habib
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 27, No 4 (2022): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ik.ijms.27.4.307-314

Abstract

Snapper is a type of demersal marine fish from the Lutjanidae family. The Lutjanidae family spread throughout the world and currently has 123 species in 21 genera, one of which is the Lutjanus genus (Miller and Thomas, 2007). To this day, the records of capture fisheries production data for snapper in Malang are still very limited to certain types. Morphological identification that has been carried out so far is still challenging to obtain accurate results because of the many similarities between the observed species or the loss of characteristics. Therefore, molecular identification is necessary to determine the types of snappers in this area and their conservation status. This study aims to determine the types of snappers using a molecular approach by Cytochrome Oxidase subunit I (COI) gene marker.  Phylogenetic tree reconstruction and genetic distance calculations were performed using Mega X software through the neighbour-joining (NJ) algorithm. The results of the identification snapper based on a molecular approach with DNA barcoding revealed that the four snapper samples were L. gibbus, L. rufolineatus, L. bengalensis, and L. erythropterus. Based on the results of the compilation of the phylogenetic tree, it can be seen that the L. bengalensis sample is closely related to L. rufolineatus while L. gibbus, and L. erythropterus each form a separate clade from the two previous Lutjanus species. Based on their conservation status at the IUCN, the four species of snapper found are in the Least Concern category.
Co-Authors Adrian Damora Ahasan Habib Alimuddin Alimuddin Dody Dharmawan Trijuno Gunarto Gunarto Laksmi Sulmartiwi Md. Jobaidul Alam Md. Jobaidul Alam Moh. Tauhid Umar Nita Rukminasari Novian Aji Pradana Reza Istiqomatul Hidayah Sapto Andriyono Yushinta Fujaya