ILMU KELAUTAN: Indonesian Journal of Marine Sciences
Vol 27, No 2 (2022): Ilmu Kelautan

Molecular Identification and Phylogenetic Trees Reconstruction of Blue Swimming Crabs (Decapoda: Portunidae) from Pangpang Bay, Banyuwangi

Sapto Andriyono (Department of Marine, Faculty of Fisheries and Marine, Universitas Airlangga)
Reza Istiqomatul Hidayah (Aquaculture Study Program, Faculty of Fisheries and Marine, Universitas Airlangga)
Laksmi Sulmartiwi (Department of Marine, Faculty of Fisheries and Marine, Universitas Airlangga)
Andi Aliah Hidayani (Fisheries Department, Faculty of Marine Science and Fisheries, Hasanuddin University)
Md. Jobaidul Alam (Department of Fisheries, Ministry of Fisheries and Livestock)



Article Info

Publish Date
05 Jun 2022

Abstract

Crabs are a group of Decapoda (Portunidae) that act as keystone species from Pangpang Bay as the marine benthic organism. Besides having an ecological function, crab also provides essential components for human health. The crab identification technique is usually conducted based on morphology and anatomy characteristics, in which certain body parts as the key for identification. This study used two identification methods, i.e. morphological features and a molecular approach. Although morphological identification has been carried out, the molecular techniques provide better accuracy and, at the same time, provide additional information about the characteristics of mitochondrial DNA. The purpose of this study is to identify the blue swimming crab caught by a traditional fisherman at Pangpang Bay, Banyuwangi, based on mitochondrial DNA sequence on cytochrome c oxidase subunit I, and reconstructed the phylogenetic tree including genetic distance also was analysed. The nucleotide sequences of the COI gene were analysed by Chromas, Clustalω, Reverse-Complement, and the MegaX. The phylogenetic tree and genetic distance calculations were carried out using Mega X software through the Neighbor-Joining (NJ) Algorithm with the addition of several sequences from the NCBI online database. This study confirmed that the specimen of Pangpang Bay is Portunus pelagicus (BWIPP001 and BWIPP003) and Portunus sanguinolentus (BWIPP002). The species of P. pelagicus have 99.99% similarities with the same species (KJ168060) from China, while the P. sanguinolentus is close to the same species (EU284144) with a per cent identity is 99.97%. The genetic distance, for P. pelagicus and P. sanguinolentus, were in range of 0.00-0.066 and 0.00-0.005 respectively.

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Journal Info

Abbrev

ijms

Publisher

Subject

Earth & Planetary Sciences

Description

ILMU KELAUTAN: Indonesian Journal of Marine Sciences (IJMS) is dedicated to published highest quality of research papers and review on all aspects of marine biology, marine conservation, marine culture, marine geology and ...