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All Journal AGRIVITA, Journal of Agricultural Science Agroekoteknologi Tropika Lembab
Sunaryo, Widi
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Agriculture, Biological Sciences & Forestry

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Induksi Multiplikasi Ubi Kayu var. Gajah (Manihot esculenta crantz) Melalui Kultur Jaringan Dengan Zat Pengatur Tumbuh BAP dan NAA Muhammad Fauzan; Ratna Nirmala; Widi Sunaryo; Penny Pujowati
Jurnal Agroekoteknologi Tropika Lembab Vol 3, No 2 (2021): Agroekoteknologi Tropika Lembab Volume 3 Nomor 2 Februari 2021
Publisher : Mulawarman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35941/jatl.3.2.2021.4813.79-85

Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh BAP dan NAA terhadap pertumbuhan eksplan ubi kayuvar.Gajah(Manihot esculenta crantz). Percobaan faktorial dalam rancangan acak lengkap dengan ulangan sebanyak tujuh kali digunakandalam penelitian. Faktor pertama yaitu konsentrasi NAA dengan dua taraf sebesar 0 ppm dan 0,5 ppm, sedangkan faktor kedua adalahkonsentrasi BAP dengan empat taraf sebesar 0 ppm, 0,5 ppm, 0,75 ppm dan 1 ppm. Data dianalisa menggunakan sidik ragam dandilanjutkan dengan Uji Beda Nyata Terkecil (BNT) dengan taraf 5%. Hasil penelitian menunjukkan bahwa BAP berpengaruh nyataterhadap tinggi tunas dan jumlah daun tetapi tidak berpengaruh nyata terhadap panjang akar tunas ubi kayu var. gajah. NAAberpengaruh nyata terhadap panjang akar tetapi tidak berpengaruh nyata terhadap tinggi tunas dan jumlah daun tunas ubi kayu var.gajah. Interaksi antara BAP dengan NAA berpengaruh nyata terhadap panjang akar tetapi tidak berpengaruh nyata terhadap tinggi danjumlah daun tunas ubi kayu var. gajah. Media MS dengan konsentrasi BAP: 1 ppm + NAA: 0,5 ppm merupakan konsentrasi terbaikuntuk induksi multiplikasi ubi kayu var. gajah.
Somatic Embryos Induction of East Kalimantan Local Rice (Oryza sativa L.) Cultivars and In Vitro Selection Against Salinity Muktirianur Muktirianur; Bambang Supriyanto; Widi Sunaryo; Nurhasanah Nurhasanah
AGRIVITA, Journal of Agricultural Science Vol 44, No 2 (2022)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v0i0.2193

Abstract

Soil salinity is one major environmental constraint on rice production, especially in coastal areas. The development of salt-tolerant genotypes is considered to be the most effective breeding strategy to overcome the constraint. This study aims to induce somatic embryos formation of East Kalimantan local rice cultivars and to obtain tolerant somatic embryos under saline condition via in vitro selection. Four commonly cultivated local rice cultivars, namely Buyung, Siam, Ketalun Tawar and Serai Gunung, were used in this study. The somatic embryos were produced using three different plant growth regulator (PGR) compositions. The salinity tolerance level of somatic embryos was induced by in vitro selection in salt toxicity medium containing 0 mM; 50 mM; 100 mM; 150 mM; 200 mM NaCl. The best medium for somatic embryogenesis contains 1 mg/l 2,4-D + 0.5 m/l  BAP, resulting the highest percentage of cream and white non-compact callus on the tested cultivars. More than 70% of the somatic embryos were tolerant against salinity (NaCl 200 mM). However, only somatic embryos derived from Serai Gunung could regenerate into normal plantlets.
Co-Authors Bambang Supriyanto Muhammad Fauzan Muktirianur Muktirianur Nurhasanah Nurhasanah Pujowati, Penny Ratna Nirmala