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All Journal Indonesian Journal of Agricultural Science Jurnal AgroBiogen
Ahmad Warsun
Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development, Jl. Tentara Pelajar 3A, Bogor 16111 Phone (62-251) 8337975
Agriculture, Biological Sciences & Forestry

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Phylogenetic and Maturity Analyses of Sixty Soybean Genotypes Used for DNA Marker Development of Early Maturity Quantitative Trait Loci in Soybean Tasma, I Made; Satyawan, Dani; Warsun, Ahmad; Yunus, Muhamad; Santosa, Budi
Jurnal AgroBiogen Vol 7, No 1 (2011): Jurnal AgroBiogen
Publisher : Jurnal AgroBiogen

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The Indonesian soybean productivity is still very low with the national average of 1.3 t/ha. One means to improve national soybean productivity is by manipulating harvest index by cultivating very early maturing soybean cultivars. Development of early maturing soybean cultivars can be expedited by using marker-aided selection. The objective of this study was to select parental lines having contrasted maturity traits and selected parents must be genetically distance. The parents then were used to develop F2 populations for detecting early maturity QTL in soybean. Maturity tests of 60 soybean genotypes were conducted at two locations, Cikeumeuh (Bogor) and Pacet (Cianjur) using a randomized block design with three replications. Genomic DNA of the 60 genotypes were analyzed using 18 SSR markers and genetic relationship was constructed using the Unweighted Pair-Group Method Arithmatic through Numerical Taxonomy and Multivariate System program version 2.1-pc. Results showed that the 60 genotypes demonstrated normal distribution in both locations for days to R1 (32-48d), days to R3 (35-55d), days to R7 (75-92d), and days to R8 (78-99d). Four early maturing genotypes and three late genotypes were obtained. Total SSR alleles observed were 237 with average allele per locus of 12.6 (3-29), and average PIC value of 0.78 (0.55-0.89). Genetic similarity among genotypes ranges from 74.8-95%. At similarity level 77% divided the genotypes into six clusters (the four selected early maturing genotypes located in clusters III and IV, while the three late genotypes located in cluster II). Based on maturity data, pubescent color, and phygenetic analysis seven parents were selected (four early maturing genotypes B1430, B2973, B3611, B4433 and three late genotypes B1635, B1658, and B3570). Twelve F2 populations were developed with the aid of SSR markers Satt300 dan Satt516. Two of the populations will be used to develop DNA markers for earliness in soybean.
IDENTIFICATION OF A MAJOR QUANTITATIVE TRAIT LOCUS CONFERRING RICE BLAST RESISTANCE USING RECOMBINANT INBRED LINES Sobrizal, Sobrizal; Bustamam, Masdiar; Carkum, Carkum; Warsun, Ahmad; Human, Soeranto; Fukuta, Yoshimichi
Indonesian Journal of Agricultural Science Vol 11, No 1 (2010): April 2010
Publisher : Indonesian Agency for Agricultural Research and Development - MOA

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Blast disease caused by Pyricularia oryzae is one of the limitingfactors for rice production world wide. The use of resistantvarieties for managing blast disease is considered as the mosteco-friendly approaches. However, their resistances may bebroken down within a few years due to the appearance of newvirulent blast races in the field. The objective of the presentstudy was to identify the quantitative trait locus (QTL) conferringresistance to blast disease using 126 recombinant inbred(RI) lines originated from a crossing of a durably resistant uplandrice genotype (Laka) and a highly susceptible rice accessioncultivar (Kencana Bali). The RI population was developedthrough a single seed descent method from 1997 to 2004.Resistance of the RI lines was evaluated for blast in an endemicarea of Sukabumi, West Java, in 2005. Disease intensity of theblast was examined following the standard evaluation systemdeveloped by the International Rice Research Institute (IRRI).At the same year the RI lines were analyzed with 134 DNAmarkers. Results of the study showed that one major QTL wasfound to be associated with blast resistance, and this QTL waslocated near RM2136 marker on the long arm of chromosome11. This QTL explained 87% of the phenotypic variation with37% additive effect. The map position of this QTL differedfrom that of a partial resistant gene, Pi34, identified previouslyon chromosome 11 in the Japanese durably resistant variety,Chubu 32. The QTL, however, was almost at the same positionas that of the multiple allele-resistant gene, Pik. Therefore, anallelic test should be conducted to clarify the allelic relationshipbetween QTL identified in this study and the Pik. The RI linesare the permanent segregating population that could be veryuseful for analysing phenotypic variations of important agronomictraits possibly owned by the RI lines. The major QTLidentified in this study could be used as a genetic resource inimprovement of rice varieties for blast resistance in Indonesia
Co-Authors Budi Santosa Carkum Carkum Dani Satyawan I Made Tasma Masdiar Bustamam Muhamad Yunus Sobrizal Sobrizal Soeranto Human Yoshimichi Fukuta