Article Per Year (5 Year)
p-Index From 2019 - 2024
0
P-Index
This Author published in this journals
All Journal Indonesian Journal of Mathematics and Natural Sciences
P Widiyaningrum
Jurusan Biologi, Fakultas MIPA, Universitas Negeri Semarang, Indonesia
Mathematics

Published : 6 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 6 Documents
Search

 1 
UJI TOKSISITAS DAN PERUBAHAN STRUKTUR MIKROANATOMI INSANG IKAN NILA LARASATI (Oreochromis nilloticus) YANG DIPAPAR TIMBAL ASETAT Mulyani, FAM; Widiyaningrum, P; Utami, NR
Jurnal MIPA Vol 37, No 1 (2014): April 2014
Publisher : Jurnal MIPA

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Penelitian ini bertujuan untuk mengetahui Nilai LC50-96 jam pada uji toksisitas ikan Nila Larasati yang dipapar timbal; mengetahui perubahan struktur  mikroanatomi dan mengetahui pada konsentrasi berapa timbal menyebabkan perubahan struktur mikroanatomi insang. Sampel uji toksisitas sebanyak 120 ekor untuk mengetahui tingkat  kematian ikan hingga 50% dalam 96 jam, dan uji perlakuan sebanyak 80 ekor dengan konsentrasi 0 ppm; 259,51 ppm; 291,94 ppm; dan 324,38 ppm. Pada akhir minggu ke empat diambil sampel insang dan dilakukan analisis gambaran struktur mikroanatomi insang secara diskriptif. Hasil analisis menunjukkan kerusakan edema 0%-25%, fusi lamela antara 1%-75%, hiperplasia 0%-50%, epithelial lifting 0%-50%, dan nekrosis 0%-50%. Dari hasil penelitian dapat disimpulkan bahwa nilai  LC50-96 jam pada uji toksisitas ikan Nila Larasati sebesar 324,38 ppm dan mulai terjadi perubahan struktur mikroanatomi insang pada konsentrasi 259,51 ppm. The objective of thje study was to determine the value of LC50-96 hours in a toxicity test of Nila Larasati fish exposed to lead acetate; to determine the changes in the microanatomy structure and to determine the concentration of lead acetate which causes microanatomy structure changes in the gills of Nila Larasati fish. The samples were 120 fish to find out the 50% fish mortality within 96 hours, and 80 fish in treatment test with the lead acetate concentrations of 0 ppm; 259.51 ppm; 291.94 ppm; and 324.38 ppm. At the end of the 4th week of the research, the gill samples were taken and the analysis of the microanatomy structure was carried out descriptively. The result of the analysis showed that there were 0%-25% edema, 1%-75% lamella fusion, 0%-50% hyperplasia, 0%-50% epithelial lifting and 0%-50% necrosis. It was concluded that the value of LC50-96 hours in Nila Larasati fish toxicity test was 324.38 ppm; and microanatomy changes started to occur in the lead acetate concentration of 259.51 ppm. 
PENURUNAN TOTAL BAKTERI DAGING AYAM DENGAN PERLAKUAN PERENDAMAN INFUSA DAUN SALAM (Syzygium polyanthum) Kusumaningrum, A; Widiyaningrum, P; Mubarok, I
Jurnal MIPA Vol 36, No 1 (2013): April 2013
Publisher : Jurnal MIPA

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Daun salam (Syzygium polyanthum) merupakan salah satu tanaman antibakteri karena mengandung senyawa flavonoid, tannin, dan minyak atsiri. Tujuan penelitian ini untuk mengetahui pengaruh perendaman infusa daun salam terhadap penurunan jumlah bakteri pada daging ayam segar post mortem. Penelitian dilakukan secara eksperimental menggunakan rancangan acak lengkap dua faktor yaitu konsentrasi infusa (0%, 5%, 10%) dan waktu pengamatan (0, 6, 12 jam setelah perendaman). Variabel yang diukur meliputi jumlah bakteri dan uji keberadaan Salmonella sp. Data total bakteri dianalisis secara kuantitatif menggunakan ANOVA serta uji lanjut BNT pada taraf uji 5%. Uji keberadaan Salmonella sp. dianalisis secara deskriptif. Penelitian ini menyimpulkan bahwa perbedaan konsentrasi infusa daun salam dapat menurunkan jumlah bakteri. Penurunan jumlah bakteri terbaik ditunjukkan pada daging ayam yang direndam pada konsentrasi 10% infusa daun salam. Hasil uji keberadaan Salmonella sp. menunjukkan hasil negatif pada semua perlakuan.  Salam leaf (Syzygium polyanthum) is one of antibacterial plants because it contains flavonoid compound, tannins, and essential oils. The purpose of this study to determine the effect of infusion soaking of salam leaf (Syzygium polyanthum) towards the decreasing of the amount of bacteria on fresh chicken meat post mortem, and also the presence test of Salmonella sp. The research was experimental research by using a complete random design with two factors of infusion concentrations (0%, 5%, 10%) and time of observation (0, 6, 12 hours after soaking). The amount of bacteria were analyzed quantitatively using ANOVA, while the presence of Salmonella sp. were analyzed descriptively. It can be concluded that the differences of concentration can reduce the amount of bacteria. The best bacteria reduction was shown in 10% salam leaf infusion and the presence of Salmonella sp. was proven negatively in all treatments.
PERKEMBANGAN OVARIUM TIKUS YANG DIPAPAR RADIASI SINAR X Nurjanah, N; Widiyaningrum, P
Jurnal MIPA Vol 39, No 2 (2016): October 2016
Publisher : Jurnal MIPA

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Penelitian ini bertujuan untuk mengetahui perkembangan ovarium tikus putih (Rattus norvegicus) galur Wistar yang dipapar radiasi sinar X, meliputi bobot dan gambaran histologisnya.  Penelitian menggunakan 20 ekor tikus betina usia 1,5 bulan dengan bobot badan rata-rata 150±13 gram.  Tikus  dikelompokkan ke dalam 4 perlakuan yaitu  perlakuan dosis radiasi sinar X sebesar 50, 100 dan 150 mGray serta satu kelompok kontrol. Variabel yang diukur meliputi bobot ovarium utuh dan gambaran histologis ovarium. Radiasi dilakukan dengan cara penyinaran satu kali tembakan pada setiap tikus per hari selama 5 hari.  Analisis data bobot ovarium dilakukan dengan dengan anova satu arah dilanjutkan dengan uji LSD pada taraf signifikansi 5%, sedangkan untuk data kualitatif gambaran histologis ovarium dianalisis secara deskriptif. Hasil analisis statistik memperlihatkan bahwa perlakuan dosis radiasi 50, 100 dan 150 mGray berpengaruh nyata terhadap bobot ovarium, yakni cenderung menurun dibanding kelompok kontrol. Pengamatan terhadap gambaran histologis ovarium memperlihatkan kerusakan ovarium mulai terjadi pada dosis 100 dan 150 mGray. Penelitian ini menyimpulkan bahwa paparan radiasi sinar X menyebabkan penurunan bobot ovarium dan pada dosis 100 mGray telah menimbulkan tanda kerusakan pada struktur histologi ovarium tikus putih galur Wistar.This study aims to determine ovarian development of rats (Rattus norvegicus) after exposure X-ray radiation, especially weights and ovarian histology. The study used 12 female rats of 1.5 months and average body weight of 150 ± 13 grams. Female rats were devided into four groups: control, and variations in dose of 50 mGray (T1), 100 mGray (T2) and 150 mGray (T3). This study used a complete randomized block design.  Radiation is done by irradiating one shot on each rat per day for 5 days. Statistical analysis for ovarian weight was performed by one way Anova and LSD Test, while qualitative data of ovarian histological were analyzed descriptively. The results showed that the variations in dose of  X-ray radiation significantly (P ≤ 5 %) of the ovarian weight. LSD test results further demonstrate that treatment of radiation doses 50, 100 and 150 mGray significant effect on ovarian weight, which decreases compared to the control group. This study concluded that exposure to X-ray radiation causes a decrease in ovarian weight and radiation dose of 100 mGray has caused damage to the structure of the ovarian histology Wistar rats.
UJI TOKSISITAS DAN PERUBAHAN STRUKTUR MIKROANATOMI INSANG IKAN NILA LARASATI (Oreochromis nilloticus) YANG DIPAPAR TIMBAL ASETAT Mulyani, FAM; Widiyaningrum, P; Utami, NR
Indonesian Journal of Mathematics and Natural Sciences Vol 37, No 1 (2014): April 2014
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Penelitian ini bertujuan untuk mengetahui Nilai LC50-96 jam pada uji toksisitas ikan Nila Larasati yang dipapar timbal; mengetahui perubahan struktur  mikroanatomi dan mengetahui pada konsentrasi berapa timbal menyebabkan perubahan struktur mikroanatomi insang. Sampel uji toksisitas sebanyak 120 ekor untuk mengetahui tingkat  kematian ikan hingga 50% dalam 96 jam, dan uji perlakuan sebanyak 80 ekor dengan konsentrasi 0 ppm; 259,51 ppm; 291,94 ppm; dan 324,38 ppm. Pada akhir minggu ke empat diambil sampel insang dan dilakukan analisis gambaran struktur mikroanatomi insang secara diskriptif. Hasil analisis menunjukkan kerusakan edema 0%-25%, fusi lamela antara 1%-75%, hiperplasia 0%-50%, epithelial lifting 0%-50%, dan nekrosis 0%-50%. Dari hasil penelitian dapat disimpulkan bahwa nilai  LC50-96 jam pada uji toksisitas ikan Nila Larasati sebesar 324,38 ppm dan mulai terjadi perubahan struktur mikroanatomi insang pada konsentrasi 259,51 ppm. The objective of thje study was to determine the value of LC50-96 hours in a toxicity test of Nila Larasati fish exposed to lead acetate; to determine the changes in the microanatomy structure and to determine the concentration of lead acetate which causes microanatomy structure changes in the gills of Nila Larasati fish. The samples were 120 fish to find out the 50% fish mortality within 96 hours, and 80 fish in treatment test with the lead acetate concentrations of 0 ppm; 259.51 ppm; 291.94 ppm; and 324.38 ppm. At the end of the 4th week of the research, the gill samples were taken and the analysis of the microanatomy structure was carried out descriptively. The result of the analysis showed that there were 0%-25% edema, 1%-75% lamella fusion, 0%-50% hyperplasia, 0%-50% epithelial lifting and 0%-50% necrosis. It was concluded that the value of LC50-96 hours in Nila Larasati fish toxicity test was 324.38 ppm; and microanatomy changes started to occur in the lead acetate concentration of 259.51 ppm. 
PENURUNAN TOTAL BAKTERI DAGING AYAM DENGAN PERLAKUAN PERENDAMAN INFUSA DAUN SALAM (Syzygium polyanthum) Kusumaningrum, A; Widiyaningrum, P; Mubarok, I
Indonesian Journal of Mathematics and Natural Sciences Vol 36, No 1 (2013): April 2013
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Daun salam (Syzygium polyanthum) merupakan salah satu tanaman antibakteri karena mengandung senyawa flavonoid, tannin, dan minyak atsiri. Tujuan penelitian ini untuk mengetahui pengaruh perendaman infusa daun salam terhadap penurunan jumlah bakteri pada daging ayam segar post mortem. Penelitian dilakukan secara eksperimental menggunakan rancangan acak lengkap dua faktor yaitu konsentrasi infusa (0%, 5%, 10%) dan waktu pengamatan (0, 6, 12 jam setelah perendaman). Variabel yang diukur meliputi jumlah bakteri dan uji keberadaan Salmonella sp. Data total bakteri dianalisis secara kuantitatif menggunakan ANOVA serta uji lanjut BNT pada taraf uji 5%. Uji keberadaan Salmonella sp. dianalisis secara deskriptif. Penelitian ini menyimpulkan bahwa perbedaan konsentrasi infusa daun salam dapat menurunkan jumlah bakteri. Penurunan jumlah bakteri terbaik ditunjukkan pada daging ayam yang direndam pada konsentrasi 10% infusa daun salam. Hasil uji keberadaan Salmonella sp. menunjukkan hasil negatif pada semua perlakuan.  Salam leaf (Syzygium polyanthum) is one of antibacterial plants because it contains flavonoid compound, tannins, and essential oils. The purpose of this study to determine the effect of infusion soaking of salam leaf (Syzygium polyanthum) towards the decreasing of the amount of bacteria on fresh chicken meat post mortem, and also the presence test of Salmonella sp. The research was experimental research by using a complete random design with two factors of infusion concentrations (0%, 5%, 10%) and time of observation (0, 6, 12 hours after soaking). The amount of bacteria were analyzed quantitatively using ANOVA, while the presence of Salmonella sp. were analyzed descriptively. It can be concluded that the differences of concentration can reduce the amount of bacteria. The best bacteria reduction was shown in 10% salam leaf infusion and the presence of Salmonella sp. was proven negatively in all treatments.
PERKEMBANGAN OVARIUM TIKUS YANG DIPAPAR RADIASI SINAR X Nurjanah, N; Widiyaningrum, P
Indonesian Journal of Mathematics and Natural Sciences Vol 39, No 2 (2016): October 2016
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Penelitian ini bertujuan untuk mengetahui perkembangan ovarium tikus putih (Rattus norvegicus) galur Wistar yang dipapar radiasi sinar X, meliputi bobot dan gambaran histologisnya.  Penelitian menggunakan 20 ekor tikus betina usia 1,5 bulan dengan bobot badan rata-rata 150±13 gram.  Tikus  dikelompokkan ke dalam 4 perlakuan yaitu  perlakuan dosis radiasi sinar X sebesar 50, 100 dan 150 mGray serta satu kelompok kontrol. Variabel yang diukur meliputi bobot ovarium utuh dan gambaran histologis ovarium. Radiasi dilakukan dengan cara penyinaran satu kali tembakan pada setiap tikus per hari selama 5 hari.  Analisis data bobot ovarium dilakukan dengan dengan anova satu arah dilanjutkan dengan uji LSD pada taraf signifikansi 5%, sedangkan untuk data kualitatif gambaran histologis ovarium dianalisis secara deskriptif. Hasil analisis statistik memperlihatkan bahwa perlakuan dosis radiasi 50, 100 dan 150 mGray berpengaruh nyata terhadap bobot ovarium, yakni cenderung menurun dibanding kelompok kontrol. Pengamatan terhadap gambaran histologis ovarium memperlihatkan kerusakan ovarium mulai terjadi pada dosis 100 dan 150 mGray. Penelitian ini menyimpulkan bahwa paparan radiasi sinar X menyebabkan penurunan bobot ovarium dan pada dosis 100 mGray telah menimbulkan tanda kerusakan pada struktur histologi ovarium tikus putih galur Wistar.This study aims to determine ovarian development of rats (Rattus norvegicus) after exposure X-ray radiation, especially weights and ovarian histology. The study used 12 female rats of 1.5 months and average body weight of 150 ± 13 grams. Female rats were devided into four groups: control, and variations in dose of 50 mGray (T1), 100 mGray (T2) and 150 mGray (T3). This study used a complete randomized block design.  Radiation is done by irradiating one shot on each rat per day for 5 days. Statistical analysis for ovarian weight was performed by one way Anova and LSD Test, while qualitative data of ovarian histological were analyzed descriptively. The results showed that the variations in dose of  X-ray radiation significantly (P ≤ 5 %) of the ovarian weight. LSD test results further demonstrate that treatment of radiation doses 50, 100 and 150 mGray significant effect on ovarian weight, which decreases compared to the control group. This study concluded that exposure to X-ray radiation causes a decrease in ovarian weight and radiation dose of 100 mGray has caused damage to the structure of the ovarian histology Wistar rats.
Co-Authors A Kusumaningrum FAM Mulyani I Mubarok N Nurjanah, N NR Utami