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All Journal Dentika Dental Journal Padjadjaran Journal of Dentistry
Haris Budi Widodo
Jenderal Soedirman University
Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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Effect of All-Trans Retinoic Acid (ATRA) against expression of Matrix Metalloproteinase-2 (MMP-2) in model mice (Rattus norvegicus) periodontitis Soraya, Ilma; Herdiana, Nadya Octoraputri; Hanggoro, Rifan; Widodo, Haris Budi
Padjadjaran Journal of Dentistry Vol 29, No 2 (2017): July
Publisher : Faculty of Dentistry Universitas Padjadjaran, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (462.526 KB) | DOI: 10.24198/pjd.vol29no2.13612

Abstract

Introduction: Periodontitis is a common chronic inflammatory disease characterised by destruction of the supporting structures of the teeth, generally caused by bacteria Phorphyromonas gingivalis (P.g). Matrix metalloproteinase-2 (MMP-2) is an enzyme that plays an important role in inflammatory conditions. All-trans retinoic acid is a metabolite of vitamin A which plays a role in healing the inflamed tissue and maintain the immune system. The purpose of this study was to determine the effect of ATRA on the expression of MMP-2 in periodontitis models of mouse Rattus norvegicus. Methods: this was a laboratory experimental study using post-test only with control group design. This study used 25 male Wistar mice that was divided into 5 groups. Group 1 is a group of healthy mice, Group 2 is a group of periodontitis induced mice without treatment, Group 3 is a group of periodontitis mice treated with 5 mg/kgBW doses of ATRA, Group 4 is a group of periodontitis mice treated with 10 mg/kgBW doses of ATRA, and Group 5 is a group of periodontitis mice treated with 20 mg/kgBW doses of ATRA. The periodontitis was induced using Phorphyromonas gingivalis bacteria every 3 days for 28 days and followed by administration of ATRA for 7 days. Expression of MMP-2 from gingival tissues and periodontal ligament was obtained by immunohistochemical methods. The results were analyzed using the Shapiro-Wilk Test and Mann-Whitney Test. Results: The results showed there were significant differences in the positive area of MMP-2 and MMP-2 color intensity (p<0.05) between Groups. Conclusion: ATRA dose of 20 mg/kgBW is the most effective dose in inhibiting the expression of MMP-2 in mice models of periodontitis when compared with other doses.
The Effect Of Ethanol Extract From Lingzhi Fungi (Ganoderma Lucidum) Cianjur Isolate On Syndecan-1 Expressions In Kb CCL17 Oral Cancer Cell: Efek Ekstrak Ethanol Dari Jamur Lingzhi (Ganoderma Lucidum) Isolat Cianjur Terhadap Ekspresi Syndecan-1 Pada Sel Kanker Rongga Mulut Kb Ccl-17 Ashar, Fadli; Widodo, Haris Budi; Wahyono, Daniel Joko; Ratnaningtyas, Nuniek Ina; Nawangtantrini, Gita; Novrial, Dody; Dwiandhono, Irfan
Dentika: Dental Journal Vol. 24 No. 2 (2021): Dentika Dental Journal
Publisher : TALENTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/dentika.v24i2.4189

Abstract

Intercellular adhesion plays a role in cancer formation and protein has a key potential in maintaining cell adhesion, including syndecan-1. Meanwhile, oral cancer originates from the oral epithelium, which has an invasive and metastatic level. Its treatments involving chemotherapy and radiotherapy commonly leave unfavorable side effects, hence, suitable alternatives are needed. Natural ingredients are widely used as an alternative treatment for cancer, for example, Ganoderma lucidum (G. lucidum) which has anti-cancer and anti-angiogenic properties, induces apoptosis, stimulates an immune response, inhibits the degradation of Extracellular matrix (ECM), reduces inflammation, affects cell cycles, cytotoxic, and acts as an antioxidant.This study aims to determine the effect of ethanol extract from Ganoderma lucidum Cianjur isolate on syndecan-1 expression in KB CCL-17 oral cell cancer. This was an experimental study with a post-test only control group design, the treatment group used G. lucidum ethanol extract with a concentration of 2.12 μg/ml (P1), 4.24 μg/ml (P2), and 8.49 μg/ml (P3), while the positive control group used cisplatin with a concentration of 11.5 μg/ml (K1). In contrast, the negative control used aquadest (K0), while syndecan-1 expression was observed using the immunohistochemical examination.The highest syndecan-1 expansion rate was found in the treatment group with a concentration of 8.49 μg/ml. A significant difference was indicated by one-way ANOVA (p<0.05) between K0 - K1, K0 - P1, K0 - P2, K0 - P3, K1 - P1, K1 - P2, K1 - P3, P1 - P2, as well as P1 and P3. The administration of ethanol extract from G. lucidum Cianjur isolate increases syndecan-1 expression in KB CCL-17 oral cell cancer.
Co-Authors Ashar, Fadli Daniel Joko Wahyono Dody Novrial, Dody Ilma Soraya, Ilma Irfan Dwiandhono Nadya Octoraputri Herdiana, Nadya Octoraputri Nawangtantrini, Gita Nuniek Ina Ratnaningtyas Rifan Hanggoro, Rifan