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All Journal The Journal of Experimental Life Sciences (JELS) Jurnal Jamu Indonesia Advances in Food Science, Sustainable Agriculture and Agroindustrial Engineering (AFSSAAE)
Widodo Widodo
Universitas Brawijaya
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Engineering Immunology & microbiology Industrial & Manufacturing Engineering Mechanical Engineering Medicine & Pharmacology Neuroscience Other

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Glucohealth: The potential of porang (Amorphophallus muelleri) acid hydrolysed glucomannan as an inhibitor of SARS-CoV-2 interaction with ACE2 Amira Hasnanuha Ulayya; Ahmad Zaki Mubarok; Widodo Widodo; Simon Bambang Widjanarko; Marlita Eklesia
Advances in Food Science, Sustainable Agriculture and Agroindustrial Engineering (AFSSAAE) Vol 4, No 2 (2021)
Publisher : Advances in Food Science, Sustainable Agriculture and Agroindustrial Engineering (AFSSAAE)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.afssaae.2021.004.02.8

Abstract

The novel SARS-CoV-2 that causing global pandemic COVID-19 known to enter the host cell using the hACE2 as cell receptor. SARS-CoV S1 protein cleaves the ACE2 receptor, then the S2 subunits facilitates the cell membrane fusion, the inhibition of S1-ACE2 interaction can help develop anti SARS-CoV-2 medication. Porang glucomannan is a polysaccharide known as immunomodulator but never reported as anti-virus by direct inhibition of viral entry. Glucohealth was developed to investigate its potential. Method: Glucohealth is a glucomannan hydrolysate (HGM) that made from porang (Amorphophallus muelleri). Hydrolysis was carried using HCl in different concentration (0.25N, 0.5N, 1N) then analyzed its inhibitor activity using ELISA kit. Result: Higher HCl concentration produced HGM with smaller average particle size and lower glucomannan content. However, ELISA studies showed that glucomannan, including its hydrolysates, have the potency to bind with S1 protein and inhibit the binding activity of S1-ACE2. Degraded glucomannan proven to have better bioactivity and able to interact with pathogen to inhibit its cell entry. This project should be a gateway for further biomedical study of glucomannan from Indonesia’s local tuber and new approach to produce more natural therapy against COVID-19.
Saponin Pada Bawang Putih Lokal Indonesia dan Potensi Biologinya (Saponins in Indonesian Local Garlic and Their Biological Potential) Balqis; Betty Lukiati; Mohammad Amin; Agus Muji Santoso; Jeni; Widodo Widodo
Jurnal Jamu Indonesia Vol. 7 No. 2 (2022): Jurnal Jamu Indonesia
Publisher : Pusat Studi Biofarmaka Tropika LPPM IPB; Tropical Biopharmaca Research Center - Bogor Agricultural University

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Abstract

This study aims to determine the various kinds of compounds from the saponin group found in local Indonesian garlic and map the biological potential of these compounds, especially the potential in the health sector. Saponins are a group of compounds characterized by the presence of foam. Saponins have various benefits both in the health, agriculture, animal husbandry, and also in the food and cosmetic industries. Fifteen compounds from the saponin group were identified from local Indonesian garlic obtained from West Papua, using Liquid Chromatography Mass Spectrometry (LCMS) and validated based on their similarity with the NCBI PubChem database. Saponin compounds in local Papuan garlic, then, their biological potential was mapped using the PASS Server and showed that there were seventeen kinds of biological potential in the saponin compound group from Indonesian local garlic. The three most dominant potencies are as anticancer, antifungal, and hypolipemic. Based on this research, it can be concluded that Indonesian local garlic has great potential to be developed and applied in the health sector, especially as an anticancer related to the saponin content in tubers.
Genetic Variation Analysis of Four Local Varieties of Indonesian Black Rice (Oryza sativa L.) Based on Partially rbcL cpDNA Gene Sequence Abdul Basith; Estri Laras Arumingtyas; Widodo Widodo
The Journal of Experimental Life Science Vol. 11 No. 1 (2021)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2021.011.01.01

Abstract

Black rice (Oryza sativa L.) varieties i.e. Toraja (South Sulawesi), Cempo Ireng (Yogyakarta), Wojalaka (East Nusa Tenggara), and Manggarai (East Nusa Tenggara) are four local black rice varieties in Indonesia whose character has not been widely studied, especially the character of genetic variation. Research aimed to determine the variation of the rbcL gene in the four local black rice varieties. The sample for testing the variation of the rbcL gene sequence in the form of black rice leaves six weeks after planting. Dendogram was carried out using the UPGMA method with the Kimura 2-parameter algorithmic calculation model using the MEGA5 version 5.2.2 program. The results showed that partially the rbcL gene sequence was successfully amplified on four black rice varieties with a sequence length of 487 bp. The partial rbcL sequence of black rice consisted of 26.58% tyrosine, 21.38% cytosine, 28.86% adenine, and 23.18% guanine. The value of G + C content was 0.446, with the frequency of invariable sites of 97.13%. The frequency of informative parsimony sites was 1.43% with a nucleotide diversity (Pi) value of 42-10, the number of haplotypes was 5, and the total number of mutations and polymorphic sites was 14. The ratio between transition and transversion (ts/tv ratio k) for purine bases was 1.741 and pyrimidine was 3.571, with the estimated overall ratio between transition and transversion (R) of 1.31. Based on the dendogram, the farthest genetic distance was found in Wojalaka and Manggarai varieties, which were 0.019 respectively.Keywords: black rice, genetic variation, local varieties, rbcL gen
Co-Authors Abdul Basith Agus Muji Santoso Ahmad Zaki Mubarok Amira Hasnanuha Ulayya Balqis Betty Lukiati Estri Laras Arumingtyas Jeni Marlita Eklesia Mohammad Amin Simon Bambang Widjanarko