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Isolasi dan Karakterisasi Bakteri Asam Laktat dari Kefir Susu Kambing Saanen (Capra aegagrus Hircus) Ronanda Rumaisha; Hendri Aldrat; Ofa Suzanthi Betha
Pharmaceutical and Biomedical Sciences Journal (PBSJ) Vol 2, No 2 (2020)
Publisher : UIN Syarif Hidayatullah Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (206.399 KB) | DOI: 10.15408/pbsj.v2i2.19970

Abstract

Kefir merupakan minuman kesehatan yang memiliki efek positif bagi manusia yang bahannya bisa berasal dari susu kambing dan sapi. Perubahan susu menjadi masam pada kefir terjadi karena adanya berbagai jenis mikroba yang hidup bersimbiosis pada bulir kefir. Sejauh ini belum banyak dilaporkan jenis BAL pada kefir yang diperoleh dari susu kambing Saanen di Indonesia. Penelitian ini bertujuan untuk mengetahui jenis dan karakteristik BAL yang terkandung dalam kefir susu kambing Saanen. BAL diisolasi dengan menggunakan media MRS yang disuplemen dengan CaCO3 1% dan diinkubasi 48 jam suhu 37oC. Selanjutnya dilakukan karakterisasi BAL dengan cara mengamati morfologi koloni dan sel, karakterisasi fisiologi dan biokimia yang di antaranya: uji katalase, motilitas, tipe fermentasi, pertumbuhan pada suhu 11oC, 37oC, dan 45oC, serta uji pertumbuhan yang disuplemen dengan NaCl 5%, 6.5%, dan 10%. Penelitian ini berhasil memperoleh 4 isolat yang sesuai dengan karakteristik umum BAL, yaitu Gram positif berbentuk bulat, tidak membentuk spora, dan bersifat katalase negatif. Semua isolat bersifat non motil, homofermentatif, dapat tumbuh pada suhu 11oC-45oC dan konsentrasi NaCl 5%-10%. Hasil karakterisasi menunjukkan keempat isolat termasuk dalam genus Enterococcus. Peran Enterococcus sebagai penghasil asam laktat didiskusikan dalam kajian ini.
Penggunaan Tumbuhan Obat Sebagai Penunjang Kesehatan pada Masyarakat Betawi Hendri Aldrat; Winda Trya Wulandari; Mita Restinia
Pharmaceutical and Biomedical Sciences Journal (PBSJ) Vol 5, No 1 (2023)
Publisher : UIN Syarif Hidayatullah Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/pbsj.v5i1.32588

Abstract

Changes in land use from rice fields and plantations to residential areas, offices, and trading complexes will certainly result in changes in the biodiversity of the environment of the inhabitants of the capital city of Jakarta, including the Betawi community. The scarcity of certain species due to land conversion also affects people’s ethnobotanical knowledge. This study aimed to explore how the Betawi people, with the rapid flow of urbanization, use medicinal plants around them. A total of 25 respondents of the selected Betawi ethnicity were interviewed about the use of medicinal plants. Purposive sampling and snowball sampling were used. The data obtained from the respondents were collected and compared using triangulation techniques with previously published ethnobotanical reports. The results showed that as many as 61 species from 36 families were used by the Betawi people to support their health. Ginger, lemongrass, and jatropha are the most widely used plants. The most widely used species belong to the Zingiberaceae family. The most commonly used part is the leaves (55%), the most common processing method is boiling (48%), the most common way to administer the medicine is by drinking (49%), and as much as 61% of medicinal plants are used in the home. In conclusion, the Betawi people still maintain the tradition of using herbal medicine amidst the high rate of urbanization in the capital city of Jakarta. Furthermore, it is necessary to conduct further experiments in the laboratory to validate the safety, efficacy, and identification of the active ingredients in these plants.