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Tri Mulyaningsih
Poltekkes Kemenkes Yogyakarta
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Pemanfaatan Pseudomonas putida untuk mendegradasi plastik Tri Mulyaningsih
Jurnal Teknologi Kesehatan (Journal of Health Technology) Vol. 17 No. 1 (2021): June
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29238/jtk.v17i1.986

Abstract

Purpose: to identify and analyze the ability of Pseudomonas sp for plastic waste degradation. Method: This study was a quasi-experimental design with post-test only with control design, using local Pseudomonas sp insulated of Piyungan landfill. Fertilizer media used BHI and selective medium for Pseudomonas Sp namely Cetrimide agar, and characterized by a series of microbact 24E kitt. Isolate elected was suspended with concentration 106CFU/ml, 107CFU/ml, and 108CFU /ml. Furthermore, distributed with volume of 10 ml suspension into each container that containing liquid of standard medium of Basal Salt and plastic (plastic black and white), with control negative (without the addition of bacteria). The incubation time for the plastic to degrade was 49 days at a temperature of 37°C, with periodic harvesting plastic every 7 days. Determination of degradation products was based on the percentage of weight loss plastic after treatment. The data analysis used anova and paired t-test to determine differences in treatment and control. Results: Results of this research showed that there were significant differences between the addition of Pseudomonas putida into media containing plastic compared to control, according to percentage of degradation.The percentage of degradation of the white plastic in the 49 days reached 3.29%, with a dose response of 108 CFU /100mg plastic while the black plastic was degraded with the percentage of degradation reached 4.7%, with a dose response of 107 CFU /100mg black plastic. Conclusion: Pseudomonas putida can degrade the plastic black or white. Percentage degradation of black plastic is greater, faster, and lower Dose response Pseudomonas putida than white plastic.
Gambaran Penyakit Demam Berdarah Dengue (DBD) di Dusun Singkil, Giring, Paliyan, Gunungkidul, Yogyakarta Aliya Nugrafitra Murti; Miftazana Firdaus Rizki Putri; Galuh Intan Novitasari; Aulia Nur Aini; Salsabila Giska Indraswari; Narto Narto; Tri Mulyaningsih
JPS : Jurnal Pengabdian Serulingmas Vol. 2 No. 1 (2022): April
Publisher : JPS : Jurnal Pengabdian Serulingmas

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (361.736 KB)

Abstract

Indonesia's health development is in the phase of re-emerging diseases. One such case is Dengue Hemorrhagic Fever (DBD), a disease transmitted by the Aedes sp. infected with the dengue virus. Based on data from the Health Office of the Special Region of Yogyakarta, the most cases of dengue fever were in Gunungkidul Regency, namely 333 patients and 2 people died. Singkil located in Giring Village, Paliyan District, is one of the hamlets in Gunungkidul that has dengue cases. Students of the D3 Sanitation study program at the Environmental Health Department of the Health Polytechnic of the Ministry of Health Yogyakarta conducted a Community Field Work Practice with several activities related to surveys, analysis, problem solving planning, socialization, and implementation of interventions regarding DHF, which aimed to reduce the transmission of DHF in the village. This activity will be held on January 3-29, 2022. The method of implementation is in the form of hands-on practice, and interviews. The data that has been obtained is processed and analyzed descriptively to describe the case. PSN activities are carried out at residents' homes. Based on the fieldwork practice, it was concluded that from making a problem map, there were 4 cases of dengue fever in 2021 and 1 case of dengue fever in 2022. The results obtained were 11 residents' houses that were positive for mosquito larvae. The provision of abate and medicinal plants was also carried out to prevent the occurrence of dengue fever.
Co-Authors Aliya Nugrafitra Murti Aulia Nur Aini Galuh Intan Novitasari Miftazana Firdaus Rizki Putri Narto Narto Salsabila Giska Indraswari