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All Journal JURNAL KAJIAN VETERINER
Yohanes T. R. M. R. Simarmata
Bagian Ilmu Penyakit Dalam Veteriner Fakultas Kedokteran Hewan, Universitas Nusa Cendana Kupang
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology Public Health Veterinary

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Identifikasi Isolat Mycobacterium bovis Dengan Konsentrasi Dna Bertingkat Menggunakan Teknik Polymerase Chain Reaction Yohanes T. R. M. R. Simarmata
JURNAL KAJIAN VETERINER Vol 2 No 2 (2014): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v2i2.1001

Abstract

Mycobacterium sp. is a bacteria that can cause tuberculosis disease in domestic animals. Mycobacterium bovis pathogens in 3rdrisk groups and Indonesia as the country's fourth-largest contributor of Tuberculosis in the world after India, China and South Africa. Based on these facts, the research was conducted to identify M. bovis with DNA stage concentrations, hope of the lowest concentration can be used as a reference for the detection of tuberculosis.Isolate DNA samples obtained from the Center for Veterinary Research (BBalitvet) in Bogor, West Java. Dilution of the DNA was started from concentration 5000 pg / mL; 2500 pg / mL; 1250 pg / mL; 625 pg / mL; 312.5 pg / mL; 156.25 pg / mL; 78.125 pg / mL and 39.0625 pg / mL . DNA amplification by PCR using two pairs of primers JB21 and JB22 Forward INS1 Reverse and Forward and Reverse INS2 with predenaturasi conditions 94 ° C for 5 min, denaturation 94 ° C for 1 min, annealing 60 ° C for 1 min, 72 ° C elongation for 1 min and post-elongation 72 ° C for 7 min at 35 cycles. PCR reaction products of 500 bp and 245bp.Analysis results can be seen in the concentration of 78.125 pg / mL and 39, 0625 pg / mL with primary INS1/INS2, bands (bands) starts to look faded and disappeared. PCR using primers JB21/JB22 showed that the concentration 39.0625 pg / mL bands had disappeared. This can be done as a source of reference for the detection of Mycobacterium bovis infection.
EFEK PENYUNTIKAN HORMON PREGNANT MARE’S SERUM GONADOTROPIN (PMSG) TERHADAP PENINGKATAN PRODUKTIVITAS INDUK BABI PADA PETERNAKAN BABI KOMERSIL DI KABUPATEN KUPANG Marianus Perada Kuslianto; Cynthia D. Gaina; Yohanes T. R. M. R. Simarmata
JURNAL KAJIAN VETERINER Vol 3 No 2 (2015): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v3i2.1042

Abstract

Pigs are one of the potential livestock to breed with the aim of meeting the needs of animal protein, but in fact the level of pig production is not optimal due to the low efficiency of reproduction. One way that can be used to increase the productivity of sows that by superovulation method. This study aimed to determine the effects of PMSG hormone injections to increase the productivity level of sows that given superovulation treatment. 10 sows that have been selected, be divided into two research groups: control group and treatment group. Before the treatment is given in the form of PMSG hormone injections, sows from both study groups, first injected with hormones PGF2α to estrus synchronization purposes. Then, sows in the treatment group are injected by PMSG hormone with the dose of 2 ml/sow before naturally mating with the boar. After the mating process, the gestation period of pregnant sow will be observed until the parturition time. The parameters were litter size and the birth weight of piglets produced from each sows of the two study groups. Sows were injected with PMSG hormone before mating show better productivity levels compared with sows in the control group. Sows were injected with PMSG hormone produce the overall number of piglets are 46 piglets or the litter size are 9 piglets with the average birth weight of 1.20 kg, while in the control group generates total number of piglets are 36 piglets or the number of litter size are 7 piglets with the average birth weight of 1.05kg. Based on statistical tests, the results showed that there’s no significantly different ((P>0.05) for the litter size of each research groups. The results showed that injection of the PMSG hormone as a superovulation agent before mating is able to increasing piglet birth weight but not able to increase the litter size significantly. Increased productivity of sows through superovulation method is to extend the production period of the sow, especially sows with a number of birth five or more times without being replace by new gilt.
Co-Authors Gaina, Cynthia Dewi Marianus Perada Kuslianto