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All Journal JURNAL KAJIAN VETERINER
Frits B. H. Francis
Fakultas Kedokteran Hewan Universitas Nusa Cendana
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology Public Health Veterinary

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Intisari Profil Enzimologi Hati Kuda sumba Sandalwood (Equus caballus) Jantan dan Betina yang dipelihara di Sumba Timur Frits B. H. Francis; Cynthia Dewi Gaina; Antin Y. N. Widi; Tri Utami; Agus Saputra
JURNAL KAJIAN VETERINER PROSIDING SEMINAR NASIONAL KE-7
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v0i0.1724

Abstract

Kuda Sandelwood merupakan aset lokal Pulau Sumba yang memiliki fungsi sosial dan ekonomi yang cukup tinggi. Profil kimia darah merupakan salah satu parameter yang dapat digunakan untuk penegakan diagnosis suatu penyakit yang dapat menyebabkan kerusakan organ, salah satunya hati. Salah satu pemeriksaan fungsi hati dapat dilakukan dengan pengujian enzim hati yang meliputi Serum Glutamic Oxaloacetic Transaminase (SGOT) atau aspartat aminotransferase (AST). Penelitian ini bertujuan untuk mengukur kadar SGOT/AST kuda Sandelwood secara umum maupun berdasarkan jenis kelamin. Pengambilan sampel dilakukan pada Desa Maubokul, Kecamatan Pandawai di Kabupaten Sumba Timur, kemudian pemeriksaan sampel dilakukan di UPT Laboratorium Kesehatan Provinsi NTT. Sampel serum darah diambil dari 20 ekor kuda Sandelwood (10 ekor jantan dan 10 ekor betina) yang sehat secara klinis kemudian serum diuji pada mesin Erba XL 300. Hasil yang didapat direratakan menggukan aplikasi SPSS 16. Hasil penelitian menunjukan kadar rata-rata SGOT kuda Sandelwood secara umum sebesar 222,30 ± 31,5 U/L, kadar rata-rata SGOT kuda jantan sebesar 212,90 ±32,05 U/L dan rata-rata SGOT kuda betina sebesar 231,70± 29,45 U/L. Hasil analisis independen t-test menunjukan tidak adanya perbedaan kadar SGOT antara kuda jantan dan kuda betina (p>0.05)
Amplifikasi DNA Kandidat Gen Kuda Pacu Sumba Cynthia Dewi Gaina; Frits B. H. Francis
JURNAL KAJIAN VETERINER Vol 9 No 1 (2021): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v9i1.3901

Abstract

The Sumba horse is one of the local horses in Indonesia which is known as racing horse Several candidate genes are known to influence the outward characteristics of the Sumba racehorse, which play main role in the development of the horse's muscles from embryo to adulthood. This research aims to identify candidate genes for the Sumba racehorse in stallion and mares. Blood samples from 5 stallions and 5 mares were collected and analyzed. The method used in this research was by using polymerase chain reaction (PCR), electrophoresis and DNA sequencing. The results of DNA amplification fragments at a temperature of 600c showed a fragment size of 463 bp. A total of 10 samples were sequenced on the PCR machine. The forward primer was 5'-TATTCTTCTTGGGAGGGAGGACTACT-3 'and reverse primer was 5'-GCAAGTAATTAGCACAAAAATTTGAATG-3'. The obtained data was analyzed using the Basic Local Alignment Sealing Tool (BLAST). Result of this study could be used as an initial identification of candidate genes for racing activity in stallion and mares that can complement the selection of racing horses.
Co-Authors Agus Saputra Antin Y. N. Widi Gaina, Cynthia Dewi Tri Utami