Article Per Year (5 Year)
p-Index From 2019 - 2024
1.431
P-Index
This Author published in this journals
All Journal HAYATI Journal of Biosciences Acta Biochimica Indonesiana Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) Journal of Microbial Systematics and Biotechnology 3BIO: Journal of Biological Science, Technology and Management
Azzania Fibriani
Department of Biology, School of Life Sciences and Technology, Bandung Institute of Technology, Bandung 40132, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Earth & Planetary Sciences Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience

Published : 8 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search
Journal : Journal of Microbial Systematics and Biotechnology

 1 
Development of a dimer-based screening system for dimerization inhibitor of HIV-1 protease I Dewa Agung Panji Dwipayana; Yana Maolana Syah; Reza Aditama; Feraliana Feraliana; Azzania Fibriani
Journal of Microbial Systematics and Biotechnology Vol 2, No 2 (2020): December 2020
Publisher : Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37604/jmsb.v2i2.42

Abstract

An in vitro dimer-based screening system (DBSS) for selecting new HIV-1 protease dimerization inhibitor candidates from natural compounds had been established. This system utilizes a fusion between HIV-1 protease and dimer binding domain of AraC protein (proteaseHIV1-AraCDBD) where fluorescence signal will be emitted in the presence of HIV-1 protease inhibitor. However, this screening system had not been evaluated. Therefore, this study was aimed to evaluate it in recombinant Escherichia coli culture. The expression of proteaseHIV1-AraCDBD fusion gene was observed for 18 hours. Its crude lysate isolation was done once every 3 hours and analyzed using SDS PAGE. To test the DBSS, darunavir was used as positive control, and Nigella sativa extract (JH3) was used as the test compound. The results of SDS PAGE analysis on crude lysates presented a ~24.2 kDa band, which was the predicted size of the proteaseHIV1-AraCDBD fusion protein based on its amino acid sequence. The growth curve and protein expression profiles revealed that the 15 hours was the optimum culture age to be used in the screening system. Darunavir testing in DBSS showed an increase in fluorescence signal compared to the negative control. The same increase in fluorescence signal was also obtained from the JH3 compound test. In conclusion, DBSS could be used as an assay to screen for new HIV-1 protease inhibitors, and the JH3 compound demonstrated the ability to inhibit HIV-1 protease dimerization.
Analysis of the SARS-CoV-2 envelope (E), nucleocapsid (N), and non-structural protein12 (nsp12) genes from COVID-19 patients in West Java Azzania Fibriani; Irin Annisa Evitayani; Gusti Ayu Prani Pradani; Rebecca Stephanie; Ema Rahmawati; Ryan Bayusantika Ristandi; Cut Nur Cinthia Alamanda; Rifky Waluyajati Rachman; Rini Robiani; Isak Solihin
Journal of Microbial Systematics and Biotechnology Vol 3, No 1 (2021): August 2021
Publisher : Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37604/jmsb.v3i1.66

Abstract

According to World Health Organization, as of January 2021, Indonesia is the only Southeast Asian country in which COVID-19 is still occurring in community transmission. West Java is one of the provinces holding the highest positive cases number. With the envelope (E), nucleocapsid (N), and non-structural protein 12 (nsp12) being the target genes of SARS-CoV-2 diagnostic kits and several antiviral drugs, the study of genetic variations has become relevant and greatly important. Out of 267 oro-nasopharyngeal swab specimens that were previously confirmed positive for COVID-19 in qPCR diagnostic test in Laboratorium Kesehatan Provinsi Jawa Barat, ten samples with acceptable qualities were selected and three samples were sequenced using Sanger sequencing. Nonsynonymous mutations were observed in the envelope gene (L21F) and in the nucleocapsid genes (R203K, G204R, A211S, and S193I). Phylogenetic analysis showed that samples were clustered with other sequences carrying identical mutations, but clustered non-discriminatively with all sequences when carrying no mutation. No pattern in geographical areas and clades, except for R203K-G204R for being a marker for the GR clade. Protein structure analysis showed that mutations observed did not change the hydrophobicity and the secondary structure of the nucleocapsid, while stability change (ΔΔG) showed that all mutations, aside from the R203K-G204R, have neutral effect on the protein stability. Therefore, it can be concluded that mutations observed in this experiment did not impart preference to disperse in certain geographical areas or cause any significant structural change in the protein.
Co-Authors Andre Hendrawan Arsyam Mawardi Cut Nur Cinthia Alamanda Ema Rahmawati Euniche R.P.F. Ramandey Feraliana Feraliana Fifi Fitriyah Masduki Gusti Ayu Prani Pradani I Dewa Agung Panji Dwipayana Irin Annisa Evitayani Isak Solihin Kenia Permata Sukma Luhung Budiailmiawan Putri Cahya Destiani Rebecca Stephanie Reza Aditama Reza Aditama Rifky Waluyajati Rachman Rini Robiani Riyanti Weni Syafitri Rosalia Rani Ryan Bayusantika Ristandi Ryan Bayusantika Ristandi Sony Suhandono Yana Maolana Syah Yeni Hotimah