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All Journal Jurnal Ilmu Farmasi dan Farmasi Klinik (Journal of Pharmaceutical Science and Clinical Pharmacy)
B.A. Martinus
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Medicine & Pharmacology

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PENETAPAN KADAR KOFEIN DALAM MINUMAN BERNERGI YANG BEREDAR DI PASARAN DENGAN METODE KROMATOGRAFI CAIR KINERJA TINGGI Afdhil Arel; B.A. Martinus; Romi Nofiandri
Jurnal Ilmu Farmasi dan Farmasi Klinik Jurnal Ilmu Farmasi & Farmasi Klinik Vol 14 No 1 juni 2017
Publisher : Universitas Wahid Hasyim Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (67.1 KB) | DOI: 10.31942/jiffk.v14i1.1767

Abstract

INTISARI                            Telah dilakukan penelitian tentang penetapan kadar kofein dalam minuman berenergi yang beredar dipasaran secara Kromatografi Cair Kinerja Tinggi. Minuman energi ini difraksinasi dengan kloroform. Hasil fraksi kloroform diuapkan dengan  rotary  evaporator. Kadar kofein diukur dengan kromatografi cair kinerja tinggi menggunakan kolom LC- 20AD dengan panjang 25 cm dan detektor SPD-20MAD dengan panjang gelombang 272nm. Fase gerak yang digunakan Metanol : Air (50:50)dengan laju alir 1 mL/menit. Dari hasil penelitian didapatkan kadar kofein dalam 1 botol sampel Kr =27,73 mg,  M =34,14 mg, , Pm=28,41 mg dan sampel Lv =69,78 mg. Hasil tersebut sampel Lv yang melebihi batas yang telah ditentukan oleh SNI No. 01-6684-2002 yaitu kurang dari 50 mg persaji. Kata Kunci : KCKT (Kromatografi cair kinerja tinggi, Koffein  ABSTRACT This research is telling about estimating amount of coffeine cosumption in energy drink which available in the market with HPLC method. Energy drink filtrat fractionated with chloroform  and chloroforom fractionation evaporated by rotary evaporator. Coffein amount measured by HPLC using column LC- 20 AD ( Length 20 cm ) and detector SPD – 20 MAD (λ 272 nm) with mobile phase methanol : water (50:50).  Amount  estimating  of  coffein  from  this  research  in  1  bottle  Sample Kr =27,7264 mg, M =34,140 mg, Pm=28,41 mg and sample Lv= 69,78 mg. The results sample Lv that exceed the limit set by SNI Num. 01-6684-2002 is less than 50 mg. Keywords: HPLC(high performance liquid chromatography), Coffeine
PENENTUAN PENGURANGAN KADAR FORMALIN PADA IKAN ASIN SEPAT DENGAN PERENDAMAN PERASAN BELIMBING WULUH DAN VARIASI SUHU AKUADES Afdhil Arel; B.A. Martinus; Halimatun Sya’diyah
Jurnal Ilmu Farmasi dan Farmasi Klinik Jurnal Ilmu Farmasi & Farmasi Klinik Vol 14 No 2 Desember 2017
Publisher : Universitas Wahid Hasyim Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (369.377 KB) | DOI: 10.31942/jiffk.v14i2.2065

Abstract

ABSTRACT This research was conducted with the aim to determine the effect of  soaking  with starfruit (Averrhoa bilimbi L) and the effect of soaking with variations in water temperature to reducing levels of formaldehyde the salted fish. Research using Spektroquant NOVA 400 on wavelength 565 nm and using reagent HCHO-1 and HCHO-2   to  know  levels  of  formaldehyde  in  the  salted  fish.  The  results   showedpercentage levels of formaldehydewithout soaking is 0%, while soaking with cold  water (28ºC  - 29ºC) is 31,62%, soaking with warm water (50ºC  -  60ºC) is 53,69%, soaking with hot water (98ºC  -  99ºC) is 62,78%, while soaking with starfruit konsentration 100% is 94,47%. This shows the soaking with water can reduce levels of formaldehyde highest with hot water, but soaking with starfruit konsentration 100% can reduce levels of formaldehyde higher of the soaking with hot water. Statistical calculation results with one-way ANOVA (p<0,05) gives results significantly  differentbetween each treatment. Keywords : Formaldehyde, Starfruit (Averrhoa bilimbi L), Salted Fish, Spektroquan NOVA 400
Co-Authors Afdhil Arel Halimatun Sya’diyah Romi Nofiandri