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All Journal Jurnal Pendidikan Matematika dan IPA Sainstek : Jurnal Sains dan Teknologi
Yossi Rahmadeni
Jurusan Biologi FMIPA Universitas Negeri Padang Jl. Prof. Dr. Hamka Air Tawar Padang, 25131
Agriculture, Biological Sciences & Forestry Chemistry Computer Science & IT Education Mathematics Physics Other

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POTENSI PSEUDOMONAD FLUORESEN ISOLAT CAS3 PADA BEBERAPA FORMULA DENGAN PENAMBAHAN STABILIZER GLISEROL DALAM MENGENDALIKAN BLOOD DISEASE BACTERIA (BDB) SECARA INVITRO Advinda, Linda; Fifendy, Mades; Rahmadeni, Yossi
Sainstek : Jurnal Sains dan Teknologi Vol 6, No 2 (2014)
Publisher : IAIN Batusangkar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (319.748 KB) | DOI: 10.31958/js.v6i2.109

Abstract

The purpose of this study was to determine the potential of fluorescent pseudomonads on some carrier with the addition of stabilizer glycerol in controlling Blood Disease Bacteria invitro. The experiment was conducted from May to July 2013, housed in the Laboratory of Plant Physiology, FMIPA UNP. Parameters measured were the amount of inhibition zone diameter fluorescent pseudomonads in controlling BDB CAS3 invitro. Observations preservation is done every 10 days until a preservation period of 60 days. This study is an experiment in completely randomized design (CRD) with 9 treatments and 3 replications. The treatments were fluorescent pseudomonads carrier CAS3 on tapioca (glycerol addition of 0,03 mL, 0,04 mL and 0,05 mL); carrier CAS3 fluorescent pseudomonads in rice flour (glycerol addition of 0,03 mL, 0,04 mL and 0,05 mL); carrier CAS3 fluorescent pseudomonads on talc (glycerol addition of 0,03 mL, 0,04 mL and 0,05 mL). Inhibition zone diameter data were analyzed using ANOVA at 5% significance level and continued with DNMRT test at 5% significance level. The results showed that the large inhibitory zone formed around the Blood Disease Bacteria vary. Results of analysis of variance on the preservation period of 10 days was significantly different at DNMRT further test, with the largest inhibition zone that is in treatment F (6,41mm) and the lowest in treatment I (1.15mm), whereas the preservation period of 20 to 60 days is not significantly different. All types of fluorescent pseudomonads carrier Cas3 potential in controlling Blood Disease Bacteria invitro.Key words : pseudomonad fluoresen, Blood Disease Bacteria, Glycerol
ANTIBACTERIAL POTENTIAL ETHANOL EXTRACT OF KAYU RACUN LEAF (Rhinacanthus nasutus) AGAINST Staphylococcus aureus AND METHICILLIN RESISTANT Staphylococcus aureus Febria, Fuji Astuti; Rahmadeni, Yossi; Bachtiar, Amri
Jurnal Pendidikan Matematika dan IPA Vol 12, No 2 (2021): July 2021
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (619.254 KB) | DOI: 10.26418/jpmipa.v12i2.46801

Abstract

The excessive use of antibiotics in the treatment of infections or diseases caused by Staphylococcus aureus is the main cause of antibiotic resistance. Methicillin-resistant S. aureus (MRSA) strains are a serious problem because of their wide distribution in the clinical environment and the living community, so it is necessary to search for antibacterial sources from natural products and traditional medicines such as Kayu racun leaves (Rhinacanthus nasutus). Information regarding the content of the active ingredient and the antibacterial potential of the ethanol extract of Kayu racun leaves is still limited. This study aims to test the phytochemical and antibacterial activity of the ethanol extract of Kayu racun leaves against S. aureus and MRSA bacteria by invitro. The research used an experimental method and was divided into two stages, namely; Phytochemical test of the ethanol extract of Kayu racun leaves using the Thin Layer Chromatography (TLC) method and the antibacterial activity test of the ethanol extract of Kayu racun leaves against S. aureus and MRSA using the paper disc diffusion method. Based on the research results, it can be concluded that the ethanol extract of Kayu racun leaves contains alkaloids, phenols, and flavonoids. The best antibacterial activity was found at a concentration of 100 mg/ml with an inhibition zone diameter of 26.20 mm against S. aureus and 17.90 mm against MRSA.
Co-Authors Amri Bachtiar Fuji Astuti Febria Linda Advinda Mades Fifendy