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All Journal Alchemy Jurnal Penelitian Kimia
M. Widyo Wartono, M. Widyo
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Chemical Engineering, Chemistry & Bioengineering Energy Environmental Science Materials Science & Nanotechnology

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AMENTOFLAVONE FROM LEAVES OF NYAMPLUNG (Calophyllum inophyllum Linn.) Wartono, M. Widyo; Wulandari, Dian; Handayani, Nestri; Suryanti, Venty; Marliyana, Soerya D.
Alchemy Jurnal Penelitian Kimia Vol 8, No 1 (2012)
Publisher : Alchemy Jurnal Penelitian Kimia

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Abstract

Amentoflavone (1), a biflavonoid was isolated from leaves of Calophyllum inophyllum Linn. Isolation and purification of the compound used maceration and chromatography methods using Si-gell and sephadex LH-20. The structure was determined by UV, IR, 1H and 13C NMR spectroscopy includes HMQC/HMBC analysis and comparison with references.
A TRAPEZIFOLIXANTHONE FROM STEM BARK OF Calophyllum soulattri COLLECTED FROM CENTRAL JAVA Achadiyah, Tri Bintari; Handayani, Nestri; Wartono, M. Widyo
Alchemy Jurnal Penelitian Kimia Vol 9, No 2 (2013)
Publisher : Alchemy Jurnal Penelitian Kimia

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A xanthone derivative namely trapezifolixanthone has been isolated and identified from EtOAc extract of stem bark ofCalophyllum soulattri. The maceration method was used to isolates the trapezifolixanthone then followed by fractionation method using vacuum liquid chromatography and purification process by flash chromatography. A 14 mg of yellowish oily solid was extracted. The compound was elucidated by 1H NMR, 13C NMR, and 2D NMR (HSQC, HMBC, COSY) supported by UV and IR spectroscopy. Base on spectral data and comparison with some references, it is  known that the compound is trapezifolixanthone.
AMENTOFLAVON DARI DAUN NYAMPLUNG (Calophyllum inophyllum Linn.) Wartono, M. Widyo; Wulandari, Dian; Handayani, Nestri; Suryanti, Venty; Marliyana, Soerya D.
Alchemy Jurnal Penelitian Kimia Vol 8, No 2 (2012)
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Satu senyawa biflavonoid yaitu amentoflavon (1) berhasil diisolasi dari daun tumbuhan nyamplung (Calophyllum inophyllum Linn). Ekstraksi menggunakan metode maserasi dan pemurnian dengan kromatografi kolom dengan fasa diam Si-gel dan sephadex LH-20. Elusidasi struktur menggunakan metode spektroskopi UV, IR, 1H dan 13 C NMR beserta HMQC dan HMBC. Hasil yang diperoleh dianalisis dan dibandingkan dengan data referensi.Kata kunci: Amentoflavon, biflavonoid, Calophyllum inophyllum, Daun
ISOLATION AND IDENTIFICATION OF ANANIXANTHONE FROM ETHYL ACETATE EXTRACT OF ROOT BARK OF SLATRI (Calophyllum soulattri) (CLUSIACEAE) Mulia, Dindha Ramah; Handayani, Nestri; Wartono, M. Widyo
Alchemy Jurnal Penelitian Kimia Vol 10, No 2 (2014)
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A xanthone, named ananixanthone (1) has been isolated and identified from the ethyl acetate extract of the root barks of Calophyllum soulattri. Structure of the compound was determined based on spectroscopic data, including UV, IR, NMR 1D, NMR 2D and by comparison with references.
IDENTIFICATION AND ANTIBACTERIAL ACTIVITY TEST OF THE MIMBA LEAF (Azadirachta Indica A. Juss) Handayani, Nestri; Wartono, M. Widyo; Murti, Riskha Kurnia
Alchemy Jurnal Penelitian Kimia Vol 8, No 1 (2012)
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The purpose of this research was to evaluate antibacterial activity of Neem (Azadirachta indica A. Juss) leaves fraction against some pathogenic bacterial and to identificate the most active fraction. Leaves powder of Neem was macerated with ethanol and factionated by vacuum liquid chromatography using hexane, ethyl acetate and ethanol as eluent, respectively. The antibacterial activity of the fraction was evaluated by diffusion method, then the most active fraction of antibacterial was evaluated by zone of inhibition. The most active fraction of antibacterial was evaluated for Minimum Inhibitory Concentration (MIC) and equivalent value, compared with amoxicillin and chloramphenicol, then identified using phytochemical screening and Gass Chromatography-Mass Spectroscopy (GC-MS).The fraction of Neem leaves fractionated by vacuum liquid chromatography had antibacterial activity againstStaphylococcus epidermidis, Bacillus cereus, and Shigella flexneri. The ethyl acetate fraction showed the most active fraction of antibacterial against all bacterial tested. Ethyl acetate fraction had MIC 0.075% againstStaphylococcus epidermidis 0.05% against Bacillus cereus and Shigella flexneri. The antibacterial activity of ethyl acetat fraction compared to amoxicillin was 0.01% for S. epidermidis, 0.02% for B. cereus, and 0.02% for S. flexneri. Then compared to chloramphenicol was 0.04% for S. epidermidis, 0.02% for B. cereus, and 0.06% for S. flexneri. The result of GC-MS showed that the ethyl acetate fraction contained palmitic acid, ethyl linoleolate, stearic acid, trans-phytol, DOP (di octyl phthalate), and suggested 1 class of compound fatty acid and 5 classes of compound triterpenoids.
Co-Authors Dian Wulandari Dindha Ramah Mulia, Dindha Ramah Nestri Handayani, Nestri Riskha Kurnia Murti, Riskha Kurnia Soerya Dewi Marliyana Tri Bintari Achadiyah, Tri Bintari Venty Suryanti