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MMUNOHISTOCHEMICAL ANALYSIS OF NF-kB (P0/P) IN PATIENT WITH AGGRESSIVE AND CHRONIC PERIODONTITIS Prahasanti, Chiquita
Indonesian Journal of Tropical and Infectious Disease Vol 4, No 4 (2013)
Publisher : Institute of Topical Disease

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (830.801 KB)

Background: Nuclear factor-kappaB (NF-ÎºB) is a protein complex that plays a role in transcription factors and in response to inflammation. Periodontitis is a periodontal disorder caused by various bacteria such as A. actinomycetemcomitan and P.gingivalis whose LPS is closely related to NFÎºB (p50/p65). Aim: This study observed whether NF-ÎºB (p50/p65) played a role in aggressive and chronic periodontitis. Methods: Data were obtained from periodontal tissue 40 patients with aggressive periodontitis and 40 patients with chronic periodontitis. Samples were derived from periodontal tissue with abnormalities and NFÎºB (p50/p65) protein expression test was performed by immunohistochemistry. The statistical test used was the t-test. Results: In NF-ÎºB (p50) the t value was -12 041 and significance 0.000, with Î± = 5%, showing significant difference in protein expression of NF-ÎºB (p50) between patients with aggressive periodontitis and chronic periodontitis. OR estimation for the value of protein expression of NFÎºB (p50) was 0.64 (sign. = 0.000). It shows that if the protein expression of NFÎºB (p50) of the respondents is incremented by 1 (one) unit, the risk of chronic periodontitis increases 1.64 times. Box plot diagram shows that the distribution of the protein expression of NFÎºB (p50) between patients with aggressive periodontitis and chronic periodontitis patients is significantly different. In NF-ÎºB (p65) the Z value was -7.137 and significance of 0000, with Î±= 5%, showed significant differences in protein expression of NF-ÎºB (p65) between patients with aggressive periodontitis and chronic periodontitis. OR estimates for protein expression of NFÎºB (p65) was 0.66 (sign. = 0.000). This indicates that if the protein expression of NFÎºB (p65) respondents is incremented by 1 (one) unit, the risk of chronic periodontitis increases 1.5 times. Box plot diagram shows that the distribution of the protein expression of NFÎºB (p65) between patients with aggressive and chronic periodontitis patients is significantly different. Conclusion: The protein expression of NF-ÎºB (p50/p65) has more influence on the incidence of chronic periodontitis patients, so it can be used as a marker for chronic periodontitis.

COMBINATION OF FRENOTOMY AND GINGIVECTOMY TREATMENT WITH LASER DIODES AS AESTHETIC CORRECTIONS: A CASE REPORT Onge Victoria Hendro; Vincent Laksono; Chiquita Prahasanti
Dentino : Jurnal Kedokteran Gigi Vol 5, No 2 (2020)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v5i2.8974

ABSTRACTBackground: High tension on the frenum in some cases may generate chronic inflammation. Chronic inflammation can trigger gingival enlargement, thereby resulting in unfavorable aesthetic appearance. Frenotomy and gingivectomy are surgical procedures that are often performed in periodontics field to overcome such condition. The use of lasers in frenectomy/frenotomy and gingivectomy methods is still uncommon but more convenient than using a scalpel. Diode lasers exhibit several advantages over conventional methods because it can control the bleeding and is also painless that may reduce the need of anesthesia. Objectives: To report the likelihood of diode lasers instead of conventional methods using scalpels in frenotomy and gingivectomy for aesthetic corrections. Case: A 20-years-old female patient arrived at Periodontics Department of Airlangga University to repair gums in upper and lower jaw region. Patient felt less confident with the state of her gum which discerned to cover the portion of her teeth and wished to receive surgical treatment for aesthetics improvement. No involvement of systemic disease and drug consumption was noted in this case. Case Management: The management of such aberrant frenum was accomplished by performing labial frenotomy and removing enlarged tissues which is a common surgical procedure in the field of periodontics. Dental lasers are currently being employed in manyfields of periodontics, providing alternatives to traditional scalpel techniques. Conclusion: Surgical treatment in cases of aberrant frenum and gingival enlargement using diode lasers (Epic X, Biolase) for aesthetic correction provides satisfactory results with minimum pain and rapid healing. Keywords: Aberrant frenum, Diode laser ,Frenotomy, Gingivectomy

Molecular detection of interleukin-1A +4845 G→T gene in aggresive periodontitis patients Chiquita Prahasanti; Harianto Notopuro
Dental Journal (Majalah Kedokteran Gigi) Vol. 45 No. 4 (2012): December 2012
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Abundant researches had been conducted based on the clinical and histopathological pathogenesis of aggresive periodontitis. Nevertheless, there were still few researches which based on molecular biology, and especially related to gene polymorphism. This study was done based on IL-1A +4845G→T gene polymorphism in aggressive periodontitis patients. Purpose: The purpose of this tudy was to characterized the generic variation of IL-1A +4845G→T as a risk factor aggressive periodontitis and chronic periodontitis. Methods: DNA from patients with aggressive periodontitis and chronic periodontitis was taken determination of IL-1A +4845 G→T polimorphism was conducted with PCR-RFLP technique. Results: Homozygous allele TT polymorphism was not found in all samples, only allele GG (wild type) and allele GT (heterozygous mutant) were not affect aggressive periodontitis and chronic periodontitis. Conclusion: The study showed there was no significant association between IL-1A +4845G→T gene polymorphism and aggressive periodontitis and chronic periodontitis. Latar belakang: Penelitian tentang patogenesa periodontitis agresif berdasar klinis dan histopatologi telah banyak dilakukan, akan tetapi penelitian berdasar biologimolekuler terutama polimorfisme gen masih sangat jarang dilakukan. Penelitian ini dilakukan berdasarkan pada polimorfisme gen IL-1A +4845G→T pada penderita periodontitis agresif. Tujuan: Tujuan dari penelitian ini adalah untuk mengetahui variasi genetik dari IL-1A +4845G→T yang merupakan faktor risiko periodontitis agresif dan periodontitis kronis. Metode: DNA dari penderita periodontitis agresif dan periodontitis kronis diisolasi, selanjutnya dilakukan determinasi dari polimorfisme gen IL-1A +4845G→T dengan menggunakan teknik PCR-RFLP. Hasil: Pada seluruh sampel penelitian ini tidak dijumpai polimorfisme allel TT (homosigot mutan), yang didapat adalah jenis allel GG (wild type) dan allel GT (heterosigot mutan) yang tidak berpengaruh terhadap periodontitis agresif dan periodontitis kronis. Kesimpulan: Polimorfisme gen IL-1A +4845G→T tidak mempunyai hubungan terhadap kejadian periodontitis agresif dan periodontitis kronis.

The effectivity of toothpick tooth brushing method on plaque control Chiquita Prahasanti; Iwan Ruhadi; Agus Sobar Mulyana
Dental Journal (Majalah Kedokteran Gigi) Vol. 44 No. 2 (2011): June 2011
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Periodontal diseases are associated with bacteria species which present in biofilms that colonize on dental surfaces. Several tooth brushing methods had been known and proved to be effective in maintaining oral hygiene. Among them, tooth pick technique was a relatively new method and its superiority in removing interproximal plaque was better than other methods. Purpose: The purpose of this study was to examine the effectivity of toothpick tooth brushing method to conventional method on periodontal health. Methods: This research was designed as an analytical observational study. Thirty samples selected from five hundred and twelve males Indonesian Air-force members in Malang, aged 18–40 yrs, with periodontal pockets (≤ 5 mm) in upper or lower teeth, without crowding, gingival index minimal > 1 (moderate gingivitis), OHI-S score minimal ≥ 1.3 (moderate), without systemic diseases, do not undergone medical therapy/drug prescriptions, without using mouth rinse during study, and without prosthesis. There were thirty samples in this research and devided to two groups, fifteen samples easch. The groups were toothpick tooth brusing method and conventional method (control group). In this study oral hygiene index simplified (OHI-S), gingival index (GI), bleeding on probing (BOP) and pocket depth were examined. Results: There were significant differences (p = .001) in OHI-S, GI, BOP, and PD before and after conducting each toothbrushing method, as well as differences between means (quarrel means), that were p = .003; p = .001; p = .001 and p = .001 consecutively. Conclusion: Toothpick brushing method was more effective in plaque control compared to conventional method.Latar belakang: Penyakit periodontal berhubungan dengan bakteri yang berkoloni dalam biofilm yang terdapat di permukaan gigi. Saat ini telah dikenal berbagai macam metode menyikat gigi tetapi masih belum ada penelitian tentang efek metode tersebut terhadap OHI-S. Penelitian in ingin menunjukkan efek menyikat gigi dengan metode toothpick terhadap kesehatan jaringan periodontal. Tujuan: Tujuan dan penelitian ini adalah untuk mengetahui efektivitas metode toothpick dibandingkan metode konvensional dalam kontrol plak. Metode: Jenis penelitian ini adalah analitik observasional. Sampel sejumlah tiga puluh orang diseleksi dari 512 anggota TNI Angkatan Udara Skadron Pasukan Khas 464 Wing II Lanud Abdulrahman Saleh Malang, usia 18–40 tahun, dengan kedalaman poket periodontal (≤ 5 mm) pada gigi rahang atas/bawah dengan, susunan gigi yang tidak berdesakan, memiliki skor gingival indeks minimal > 1 (gingivitis sedang), memiliki skor OHI-S minimal ≥ 1,3 (sedang), tanpa ada kelainan sistemik, tidak dalam perawatan dokter/mengkonsumsi obat-obatan, tidak menggunakan obat kumur selama penelitian, tidak menggunakan protesa. Sampel dibagi menjadi dua kelompok, lima belas orang menggunakan metode toothpick sedangkan lima belas orang menggunakan metode konvensional sebagai kelompok kontrol. Hasil: Terdapat perbedaan yang bermakna (p = .001) pada OHI-S, GI, BOP dan kedalaman poket sebelum dan sesudah menyikat gigi dengan masing-masing metode menyikat gigi yang ditetapkan. Selain itu, terdapat perbedaan bermakna antara selisih rerata sebelum dan sesudah menyikat gigi (p = .003; p = .001; p = .001 and p = .001) antara kedua kelompok tersebut. Kesimpulan: Metode toothpick brushing lebih efektif uuntuk kontrol plak dibanding dengan kelompok kontrol.

Multidisciplinary management of a mandibular buccal plate perforation Yuli Nugraeni; Chiquita Prahasanti
Dental Journal (Majalah Kedokteran Gigi) Vol. 43 No. 4 (2010): December 2010
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Endodontists often have difficulty in the management of endo-perio cases, because they cannot visually detect the condition of outer root surfaces, especially in bucco-lateral roots. The bone defect is rare and its treatment needs collaboration of endodontists and periodontists. An endodontist treats cases based on dental history, radiograph of root canals to measure root canals, to uncover abnormalities and to diagnose, but as the endodontists cannot directly see the affected parts, the unseen portion of the tooth could only be seen after flap surgery. Purpose: This case presents the importance of multidisciplinary approach by the endodontist and periodontitis to treat bucal plate perforations in endo-perio cases. Case: The first patient, a 47-year-old female had endodontic treatment and a porcelain crown restoration; however, after several months she felt pain. The second patient, a 45 year-old female had endodontic treatment and after six months she feel painful. Case management: The first patient, was referred to a periodontist. The opening flap surgery has been done, a bone defect was found in tooth 45. Subsequently, the exposed crater was filled with a bone graft and the pain disappeared. The second patient, with improper endodontic treatment. The flap surgery was conducted, there was found a bone defect in tooth 36. The last treatment, a bone graft has been covered and then the pain was not present. Conclusion: Proper diagnosis and treatment of perforations on the buccal aspect of a root was able to eliminate pain and avoid tooth extraction.Latar belakang: Endodontis sering mengalami kesulitan dalam menangani kasus endo-perio karena secara visual kondisi ini tidak tampak diluar permukaan akar, khususnya pada akar bukolateral. Defek tulang sangatlah jarang dan perawatannya membutuhkan kolaborasi endodotis dan periodontis. Seorang endodontis merawat kasus-kasus berdasarkan riwayat gigi, radiografis saluran akar untuk melihat saluran akar, melihat abnormalitas dan diagnosis, tetapi ketika endodontis tidak dapat secara langsung melihat bagian yang terlibat, bagian gigi yang tidak terlihat ini hanya dapat dilihat setelah dilakukan bedah flap. Tujuan: Kasus ini menunjukkan pentingnya pendekatan multidisipliner oleh endodontis dan periodontis dalam menangani perforasi aspek bukal akar pada kasus endoperio. Kasus: Pasien pertama, seorang wanita 47 tahun telah mendapatkan perawatan endodontik dan restorasi mahkota porselen, akan tetapi selama beberapa bulan pasien tersebut mengeluh nyeri. Pasien kedua, seorang wanita 45 tahun telah mendapatkan perawatan endodontik dan setelah 6 bulan juga mengeluh nyeri pada gigi tersebut. Tatalaksana kasus: Pasien pertama dirujuk ke periodontis. Bedah pembukaan flap dilakukan dan ditemukan defek tulang pada gigi 45, kemudian crater yang terbuka diisi graft tulang dan nyeri berangsur hilang. Pada pasien kedua ditemukan perawatan endodontik yang kurang baik. Pada pasien ini ilakukan bedah flap dan ditemukan defek tulang pada gigi 36, pada akhir perawatan graft tulang dipasang dan nyeri berangsur hilang. Kesimpulan: Diagnosa dan perawatan perforasi yang tepat pada aspek bukal akar dapat menghilangkan nyeri dan menghindari ekstraksi gigi.

Interleukin-1β expression on periodontitis patients in Surabaya Chiquita Prahasanti
Dental Journal (Majalah Kedokteran Gigi) Vol. 43 No. 4 (2010): December 2010
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Periodontal disease, commonly known as periodontitis is an infectious disease which has multifactorial etiologic factors. It may affect everybody in any ages with no gender nor sex predilection and usually can be detected under routine clinical examination. This disease is a manifestation of local factors, host factor and environmental factors, resulting in periodontal tissue damage which may cause tooth mobility and tooth loss. Interleukin-1 is a pro-inflammatory protein which functions primarily as inflammatory mediator in host innate immune responses. IL-1 is a regulator, affecting many biological activities including proliferation, development, homeostasis, regeneration, repair and inflammation which contribute to tissue damage and alveolar bone resorption. Purpose: This research was aimed to reveal the basic pathogenesis of periodontitis and could determine the future definitive treatment for patients with periodontitis. Methods: Data were obtained from 40 patients with aggressive periodontitis and 40 patients with chronic periodontitis. Samples were collected from periodontal tissue patients and protein expression of IL-1β was performed with immunohistochemistry. Results: Most female patient suffer aggressive periodontitis and chronic periodontitis. The datas were analyzed with t-test. The t values result was -8623, significance 0.001, with α = 5%, which indicated there was significant difference in IL-1β expression between aggressive and chronic periodontitis. The box plot diagram showed marked difference in distribution of protein expression of IL-1β between patients with aggressive periodontitis and chronic periodontitis. With a regression equation, it might be concluded that the protein expression of IL-1β might affect the incidence of aggressive periodontitis and chronic periodontitis. The OR value was calculated for 0.746 (sign.= 0.001), which indicate each increment of one unit protein expression of IL-1β will lead the risk for Aggressive periodontitis 0.746 times higher or if the protein expression of respondents increased one unit, the risk of chronic IL-1β periodontitis may be 1.34 times higher. Conclusion: This study elucidated that the elevation proteins expression of IL-1β in patients with chronic periodontitis demonstrated this cytokine as an indicator of inflammation.Latar belakang: Penyakit periodontal yang biasa dikenal dengan periodontitis adalah penyakit infeksi, yang disebabkan oleh berbagai faktor, dapat menyerang setiap orang tanpa membedakan usia dan gender serta mudah ditemukan pada pemeriksaan klinis oleh seorang dokter gigi. Penyakit ini merupakan manifestasi dari interaksi antara faktor lokal dengan faktor lingkungan, yang berakibat pada kerusakan jaringan periodontal, dapat mengakibatkan terjadinya kegoyangan gigi hingga tanggalnya gigi. Interleukin-1 merupakan protein pro-inflamatori dengan fungsi utama sebagai mediator respon inflamasi pejamu pada sistem imunitas innate. Interleukin-1 merupakan regulator, dimana memainkan peranan pada sejumlah aktivitas biologic termasuk proliferasi, pengembangan, homeostasis, regenerasi, repair dan keradangan berperan pada kerusakan jaringan ikat serta resorpsi tulang alveolar. Tujuan: Penelitian ini bertujuan untuk menentukan dasar patogenesa periodontitis dan dapat digunakan sebagai dasar perawatan penderita periodontitis pada masa mendatang. Metode: Data penelitian didapat dari 40 penderita dengan periodontitis agresif dan 40 penderita periodontitis kronis. Sampel berasal dari jaringan yang mengalami kelainan periodontal dan uji ekspresi protein IL-1β dilakukan secara imunohistokimia. Hasil: Penderita yang mengalami kelainan pada penelitian ini sebagian besar adalah perempuan baik periodontitis agresif maupun periodontitis kronis. Uji statistik yang digunakan adalah uji-t diperoleh nilai t sebesar -8.623 dan signifikansi 0.001, dengan α = 5% maka terdapat perbedaan bermakna ekspresi protein IL-1β antara penderita periodontitis agresif dan penderita periodontitis kronis. Diagram box plot memperlihatkan sebaran ekspresi protein IL-1β antara penderita periodontitis agresif dan penderita periodontitis kronis yang tampak sangat jauh berbeda. Ekspresi protein IL-1β berpengaruh pada kejadian penderita periodontitis agresif dan penderita periodontitis kronis, dengan bentuk persamaan regresi. Nilai estimasi OR untuk variabel ekspresi protein IL-1β adalah 0,746 (sign. = 0,00). Artinya, jika ekspresi protein IL-1β responden bertambah satu satuan, maka risiko terjadinya periodontitis agresif menjadi 0,746 kali atau jika ekspresi protein IL-1β responden bertambah satu satuan, maka risiko terjadinya periodontitis kronis menjadi 1,34 kali. Kesimpulan: Ekspresi protein Il-1β yang meningkat pada penderita periodontitis kronis menujukkan bahwa sitokinini merupakan indikator pada keadaan keradangan.

Chronic gingivitis and aphthous stomatitis relationship hypothesis: A neuroimmunobiological approach Chiquita Prahasanti; Nita Margaretha; Haryono Utomo
Dental Journal (Majalah Kedokteran Gigi) Vol. 42 No. 1 (2009): March 2009
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Traumatic injuries to the oral mucosa in fixed orthodontic patients are common, especially in the first week of bracket placement, and occasionally lead to the development of aphthous stomatitis or ulcers. Nevertheless, these lesions are selflimiting. Purpose: The objective of this study is to reveal the connection between chronic gingivitis and aphthous stomatitis which is still unclear. Case: A patient with a persistent lesion for more than six months. Case Management: RAS was treated with scaling procedure, the gingival inflammation was healed. However, in this case report, despite the appropriate management procedures had been done, the lesion still worsen and became more painful. Moreover, the symptoms did not heal for more than two weeks. Actually, they had been undergone orthodontic treatment more than six months and rarely suffered from aphthous stomatitis. Coincidentally, at that time they also suffered from chronic gingivitis. It was interesting that after scaling procedures, the ulcer subsides in two days. Conclusion: Recently, the neuroimmunobiological researches which involved neurotransmitters and cytokines on cell-nerve signaling, and heat shock proteins in gingivitis and stomatitis are in progress. Nevertheless, they were done separately, thus do not explain the interrelationship. This proposed new concept which based on an integrated neuroimmunobiological approach could explain the benefit of periodontal treatment, especially scaling procedures, for avoiding prolonged painful episodes and unnecessary medications in aphthous stomatitis. However, for widely acceptance of the chronic gingivitis and aphthous stomatitis relationship, further clinical and laboratory study should be done. Regarding to the relatively fast healing after scaling procedures in this case report; it was concluded that the connection between chronic gingivitis and aphthous stomatitis is possible.

Reducing allergic symptoms through eliminating subgingival plaque Haryono Utomo; Chiquita Prahasanti; Iwan Ruhadi
Dental Journal (Majalah Kedokteran Gigi) Vol. 41 No. 4 (2008): December 2008
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Elimination of subgingival plaque for prevention and treatment of periodontal diseases through scaling is a routine procedure. It is also well-known that periodontal disease is related to systemic diseases. Nevertheless, the idea how scaling procedures also able to reduce allergic symptoms i.e. eczema and asthma, is not easily accepted, because it is contradictory to the “hygiene hypothesis”. However, since allergic symptoms also depend on variable factors such as genetic, environmental and infection factors; every possible effort to eliminate or avoid from these factors had to be considered. Subgingival plaque is a source of infection, especially the Gram-negative bacteria that produced endotoxin (lipopolysaccharides, LPS), a potential stimulator of immunocompetent cells, which may also related to allergy, such as mast cells and basophils. In addition, it also triggers the “neurogenic switching” mechanism which may be initiated from chronic gingivitis. Objective: This case report may explain the possible connection between subgingival plaque and allergy based on evidence-based cases. Case: Two adult siblings who suffered from chronic gingivitis also showed different manifestations of allergy that were allergic dermatitis and asthma for years. They were also undergone unsuccessful medical treatment for years. Oral and topical corticosteroids were taken for dermatitis and inhalation for asthma. Case Management: Patients were conducted deep scaling procedures, allergic symptoms gradually diminished in days even though without usual medications. Conclusion: Concerning to the effectiveness of scaling procedures which concomitantly eliminate subgingival plaque in allergic patients, it concluded that this concept is logical. Nevertheless, further verification and collaborated study with allergic expert should be done.

Toll-like receptor–4 gene polymorphisms in Javanese aggressive and chronic periodontitis patients Chiquita Prahasanti
Dental Journal (Majalah Kedokteran Gigi) Vol. 48 No. 4 (2015): December 2015
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Tool-like receptor-4 (TLR4) gene polymorphisms affect the ability of the host in response to pathogenic bacteria, and can also be associated with the severity of periodontitis. TLR4 gene polymorphisms (Asp299Gly and Thr399Ile) are ones of gene mutations that occur in patients with aggressive periodontitis. Purpose: To investigate the involvement of TLR4 gene polymorphism as a risk factor of aggressive and chronic periodontitis of Javanese population in Surabaya. Method: This research can be considered as an analytic observational study, with a case-control study design in patients with aggressive periodontitis and chronic periodontitis. DNA samples were derived from peripheral blood. TLR4 gene polymorphisms (Asp299Gly and Thr399Ile) were then observed by PCR-RFLP. Result: There was no TLR4 gene polymorphism (Asp299Gly) in the whole samples. And, based on the results of simple logistic regression analysis on TLR4 gene polymorphisms (Thr399Ile), mutants heterozygote and homozygote obtained had OR value about 0.25. Conclusion: In Surabaya, there was no heterozygote and homozygote mutant in TLR4 gene polymorphisms, (Asp299Gly) and (Thr399Ile), that can be considered as risk factors of chronic periodontitis.

MMUNOHISTOCHEMICAL ANALYSIS OF NF-kB (P0/P) IN PATIENT WITH AGGRESSIVE AND CHRONIC PERIODONTITIS Chiquita Prahasanti
Indonesian Journal of Tropical and Infectious Disease Vol. 4 No. 4 (2013)
Publisher : Institute of Topical Disease Universitas Airlangga

Background: Nuclear factor-kappaB (NF-κB) is a protein complex that plays a role in transcription factors and in response to inflammation. Periodontitis is a periodontal disorder caused by various bacteria such as A. actinomycetemcomitan and P.gingivalis whose LPS is closely related to NFκB (p50/p65). Aim: This study observed whether NF-κB (p50/p65) played a role in aggressive and chronic periodontitis. Methods: Data were obtained from periodontal tissue 40 patients with aggressive periodontitis and 40 patients with chronic periodontitis. Samples were derived from periodontal tissue with abnormalities and NFκB (p50/p65) protein expression test was performed by immunohistochemistry. The statistical test used was the t-test. Results: In NF-κB (p50) the t value was -12 041 and significance 0.000, with α = 5%, showing significant difference in protein expression of NF-κB (p50) between patients with aggressive periodontitis and chronic periodontitis. OR estimation for the value of protein expression of NFκB (p50) was 0.64 (sign. = 0.000). It shows that if the protein expression of NFκB (p50) of the respondents is incremented by 1 (one) unit, the risk of chronic periodontitis increases 1.64 times. Box plot diagram shows that the distribution of the protein expression of NFκB (p50) between patients with aggressive periodontitis and chronic periodontitis patients is significantly different. In NF-κB (p65) the Z value was -7.137 and significance of 0000, with α= 5%, showed significant differences in protein expression of NF-κB (p65) between patients with aggressive periodontitis and chronic periodontitis. OR estimates for protein expression of NFκB (p65) was 0.66 (sign. = 0.000). This indicates that if the protein expression of NFκB (p65) respondents is incremented by 1 (one) unit, the risk of chronic periodontitis increases 1.5 times. Box plot diagram shows that the distribution of the protein expression of NFκB (p65) between patients with aggressive and chronic periodontitis patients is significantly different. Conclusion: The protein expression of NF-κB (p50/p65) has more influence on the incidence of chronic periodontitis patients, so it can be used as a marker for chronic periodontitis.

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