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All Journal Buletin Kebun Raya Jurnal Jejaring Matematika dan Sains
Yupi Isnaini, Yupi
Pusat Konservasi Tumbuhan Kebun Raya, Lembaga Ilmu Pengetahuan Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Mathematics Physics

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PERAN PELUKAAN ALAMI DALAM PEMBENTUKAN GUBAL GAHARU: STUDI KASUS PADA Aquilaria malaccensis Lamk. KOLEKSI KEBUN RAYA BOGOR Isnaini, Yupi
Buletin Kebun Raya Vol 11, No 1 (2008): Buletin Kebun Raya Vol. 11 (1) Januari 2008
Publisher : Center for Plant Conservation Bogor Botanic Garden, Indonesian Institute of Sciences

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Abstract

PERKECAMBAHAN BIJI KANTONG SEMAR (Nepenthes gracilis Korth.) SECARA IN VITRO Isnaini, Yupi; Handini, Elizabeth
Buletin Kebun Raya Vol 10, No 2 (2007): Buletin Kebun Raya Vol. 10 (2) Juli 2007
Publisher : Center for Plant Conservation Bogor Botanic Garden, Indonesian Institute of Sciences

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Abstract

Mature seeds of a pitcher plant (Nepenthes grocilis Korth.) were cultured in seven different media, namely half strength MS medium, half strength MS medium supplemented with BAP and NAA, 0.8 KC medium supplemented with coconut water + bean sprout extract, 0.8 KC + H3B03 + CuSO3 + ZnSO4 + coconut water + bean sprout extract, 0.25 KC + Vitamin (MS), HYPONeX + coconut water + sprout bean extract, and HYPONeX + potato extract. Each culture was incubated under light conditions. Young seedlings were subsequently transferred onto fresh media of 0.5 MS and 0.25 KC supplemented with 0, 5, 10, and 15 mg/I GA3 after 15 weeks of sowing. Seed germination and seedling growth were observed weekly. It was noted that the seeds started to germinate in ten weeks after incubation and the highest seed germination was obtained in HYPONeX medium + potato extract. However, the appearance of the seedlings was better on MS media and KC media without organic compound than on the other media. Seedlings in all level of GA3 in half strength MS media and 0.25 KC media grew faster than those in media without GA3.
PERKECAMBAHAN BIJI KANTONG SEMAR (Nepenthes gracilis Korth.) SECARA IN VITRO Isnaini, Yupi; Handini, Elizabeth
Buletin Kebun Raya Vol 10, No 2 (2007): Buletin Kebun Raya Indonesia Vol. 10 (2) July 2007
Publisher : Center for Plant Conservation Bogor Botanic Garden, Indonesian Institute of Sciences

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Abstract

Mature seeds of a pitcher plant (Nepenthes grocilis Korth.) were cultured in seven different media, namely half strength MS medium, half strength MS medium supplemented with BAP and NAA, 0.8 KC medium supplemented with coconut water + bean sprout extract, 0.8 KC + H3B03 + CuSO3 + ZnSO4 + coconut water + bean sprout extract, 0.25 KC + Vitamin (MS), HYPONeX + coconut water + sprout bean extract, and HYPONeX + potato extract. Each culture was incubated under light conditions. Young seedlings were subsequently transferred onto fresh media of 0.5 MS and 0.25 KC supplemented with 0, 5, 10, and 15 mg/I GA3 after 15 weeks of sowing. Seed germination and seedling growth were observed weekly. It was noted that the seeds started to germinate in ten weeks after incubation and the highest seed germination was obtained in HYPONeX medium + potato extract. However, the appearance of the seedlings was better on MS media and KC media without organic compound than on the other media. Seedlings in all level of GA3 in half strength MS media and 0.25 KC media grew faster than those in media without GA3.
PERAN PELUKAAN ALAMI DALAM PEMBENTUKAN GUBAL GAHARU: STUDI KASUS PADA Aquilaria malaccensis Lamk. KOLEKSI KEBUN RAYA BOGOR Isnaini, Yupi
Buletin Kebun Raya Vol 11, No 1 (2008): Buletin Kebun Raya Indonesia Vol. 11 (1) January 2008
Publisher : Center for Plant Conservation Bogor Botanic Garden, Indonesian Institute of Sciences

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Abstract

Konservasi In Vitro Kantong Semar (Nepenthes rafflesiana Jack.) dengan Metode Slow Growth Previaningrum, Hanum; Qadir, Abdul; Isnaini, Yupi
Jurnal Jejaring Matematika dan Sains Vol. 3 No. 1 (2021): Vol. 3 No. 1 (2021): Edisi Juni 2021
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Palangka Raya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36873/jjms.2021.v3.i1.502

Abstract

Nepenthes rafflesiana Jack. is one of a unique plant because there is the pitcher on the tips of the leaves to trap insects. This uniqueness makes N. rafflesiana mostly taken from its natural habitat for sale. Big Explored from nature caused extinction and ecosystem habitats damaged. Efforts to prevent extinction through ex situ conservation by in vitro culture of N. rafflesiana with slow growth method. This study aims were to determine the effect of paclobutrazol on the growth of N. rafflesiana and to obtain the most suitable concentration of paclobutrazole for in vitro conservation of N. rafflesiana. The research was conducted at the Tissue Culture Laboratory of the Research Center for Plant Conservation and Botanical Gardens from January to April 2020. The experiment was carried out in a Completely Randomized Group Design with a single factor, namely the concentration of paclobutrazol: 0, 1, 3 and 5 ppm. The results showed that the best concentration of paclobutrazol for in vitro conservation of N. rafflesiana was 5 ppm which could inhibit the growth of stem height and number of leaves and could maintain the green color of the leaves.
Co-Authors Abdul Qadir Elizabeth Handini Previaningrum, Hanum