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A. Sudiro, A.
Karya Anugerah Rumpin (PT. KAR), Jl. Raya Cibodas No. 99, Rumpin, West Java
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences

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Journal : Journal of the Indonesian Tropical Animal Agriculture

 1 
Association of growth hormone (GH) gene polymorphism with growth and carcass in Sumba Ongole (SO) cattle Agung, P. P.; Anwar, S.; Putra, W. P. B.; Zein, M. S. A.; Wulandari, A. S.; Said, S.; Sudiro, A.
Journal of the Indonesian Tropical Animal Agriculture Vol 42, No 3 (2017): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.42.3.153-159

Abstract

A study was conducted to identify the polymorphism in the intron 3 of the Growth Hormone (GH) gene and also to evaluate the association of the GH gene polymorphism with growth parameters and dressing percentage in the Sumba Ongole (SO) cattle. A total of 267 individual DNA samples were used in the Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) analysis. The SO cattle growth parameters data (n=44) including birth weight (BW), weaning weight at 205 days of age (WW205), yearling weight at 365 days of age (YW365) and also dressing percentage (DP) (n=122) were investigated in this study. There were three genotypes (AA, AB, and BB) of the GH gene based on the PCR-RFLP analysis with allele frequency was 0.87 and 0.13 for A allele and B allele respectively. The highest genotype frequency in the SO cattle is AA (0.76) and the lowest is BB (0.02). The Heterozygosity Observed (Ho) value in the SO cattle population is 0.23 and Polymorphism Information Content (PIC) value is 0.20. Therefore, the genetic diversity in the SO cattle based on the GH gene polymorphism is quite low. There is no association (P>0.05) in BW, WW205, YW365, and DP with genotypes of the GH gene. As the result, the GH gene in this study cannot be used as a genetic marker in the SO cattle breeding program.
ESTIMATION OF MOST PROBABLE PRODUCING ABILITY VALUE FOR CALF BIRTH'S PERFORMANCE IN SUMBA ONGOLE COWS Said, S.; Agung, P. P.; Putra, W. P. B.; Anwar, S.; Wulandari, A. S.; Sudiro, A.
Journal of the Indonesian Tropical Animal Agriculture Vol 41, No 2 (2016): June
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.41.2.53-60

Abstract

An estimation of most probable producing ability (MPPA) value based on the calves performance is very important for the cow selection. The birth data record of 48 Sumba Ongole (SO) cows and record data from 52 callf birth’s performance were used to estimate the value of MPPA. High of r value (r > 0.30) was obtained at the body length (BL). Birth weight (BW), withers high (WH) and chest girth (CG) had a moderate value (0.10 < r < 0.30) of repeatability. The MPPA value of the cumulative calf birth’s performance of cows based on the highest birth records was 4.64 (cow number 3770) and the lowest was -4.64 (cow number 2283). Calf number B2076 (cow number 3586) had the highest ratio of WH (115.70); BL (124.26); CG (118.90), while the highest ratio of BW (168.35) was obtained in calf number B2095 (cow number 3731). It was concluded that the BW of birth had a moderate value of r (0.10) and could be used as a selection criteria accurately. 
SELECTION OF SUMBA ONGOLE (SO) CATTLE BASED ON BREEDING VALUE AND PERFORMANCE TEST Said, S.; Agung, P. P.; Putra, W. P. B.; Anwar, S.; Wulandari, A. S.; Sudiro, A.
Journal of the Indonesian Tropical Animal Agriculture Vol 41, No 4 (2016): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.41.4.175-187

Abstract

Selection of livestock was purposed to increase productivity. A performance test was conducted using 25 males and 25 females of Sumba Ongole (SO) cattle. The performance test were conducted in three periods (2014, 2015 and 2016) for about 535 days. Descriptive statistics ws used to analyze data of body weight. Cattle with age between 300 to 600 days were used for evaluation. Result showed that heritability value of yearling weight (YW365) was 0.77+0.68 as a high category. The highest breeding value of YW365 were 66.05 kg (male) and 41.89 kg (female). The average of corrected final weight (CFW) weight were 172.55+34.22 kg (male) and 159.80+37.73 kg (female). The performance test in this study obtained six A class (1 bull and 5 cows) based on the standard minimum of body measurements for SO cattle.
MYOSTATIN GENE ANALYSIS IN THE FIRST GENERATION OF THE BELGIAN BLUE CATTLE IN INDONESIA Agung, P. P.; Said, S.; Sudiro, A.
Journal of the Indonesian Tropical Animal Agriculture Vol 41, No 1 (2016): March
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.41.1.13-20

Abstract

A study was conducted to identify the variations of the myostatin and also to evaluate the existence of genetic marker for “double muscling” (11-bp deletion in the third exon of the myostatin gene) in the first generation of Belgian Blue cattle in Indonesia using the Single Strand Conformation Polymorphism (SSCP) and the sequencing analysis. A total of 8 DNA samples belonged to Karya Anugerah Rumpin (KAR) Farm were used in the Single Strand Conformation Polymorphism (SSCP) and the sequencing analysis. There were 3 allele types of myostatin gene based on the SSCP analysis. The Belgian Blue sire has type A allele. The Simmental, Wagyu, SO x BX, Charolais, and the PO cattle have the type B allele, while the Belgian Blue x FH and the Belgian Blue x SO have the type C allele (heterozygous). There are 11-bp deletion in the third exon myostatin gene for the Belgian Blue sire based on the sequencing analysis. The myostatin gene in the Belgian Blue F1 generation individual was heterozygous. This study provides scientific evidence that the 11-bp deletion in the third exon of myostatin gene in the Belgian Blue sire was inherited to its F1 generation (male and female).
THE POTENCY OF SUMBA ONGOLE (SO) CATTLE: A STUDY OF GENETIC CHARACTERIZATION AND CARCASS PRODUCTIVITY Agung, P. P.; Anwar, S.; Wulandari, A. S.; Sudiro, A.; Said, S.; Tappa, B.
Journal of the Indonesian Tropical Animal Agriculture Vol 40, No 2 (2015): June
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.40.2.71-78

Abstract

A study was conducted to assess the genetic characterization of the Sumba Ongole (SO) cattlebased on DNA microsatellites and also to study the potency of SO cattle based on carcass productivity.Blood samples were collected from 28 individual cattle and 12 microsatellite primers as recommendedby FAO were used to identify the genetic characterization of the SO cattle population. Data of carcassproductivity were collected from 506 individual cattle that slaughtered in Karawaci abattoir, Banten,Indonesia. The heterozygosity values of microsatellite loci ranged from 0.143 to 1.000 (mean 0.536).The highest PIC values was 0.814 (locus TGLA122), while the lowest was 0.280 (locus BM1818).Cattle in range of 351-475 kg slaughter weight was most slaughtered in year 2013 and 2014 with carcasspercentage ranged from 52.89% to 53.43%. The highest carcass percentage (56.34%) was obtained fromcattle in range of 626-650 kg slaughter weight while the lowest (51.42%) was obtained from cattle inrange of 250-275 kg slaughter weight. The results of genetic characterization showed that allmicrosatellite locus were highly polymorphic and highly informative for detecting the level of geneticdiversity in the SO cattle population. The results of carcass productivity showed that the SO cattle hasexcellent potential as beef cattle compare with other local breeds cattle in Indonesia.
Co-Authors A. S. Wulandari, A. S. B. Tappa M. S. A. Zein, M. S. A. P. P. Agung, P. P. S. Anwar S. Said W. P. B. Putra