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S. Said
Research Center for Biotechnology - Indonesian Institute of Sciences, Jl. Raya Bogor KM. 46, Cibinong 16911, West Java
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences

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STUDY ON CHANGES OF SPERM HEAD MORPHOMETRY AND DNA INTEGRITY OF FREEZE-DRIED BOVINE SPERMATOZOA Said, S.; Afiati, F.; Maulana, T.
Journal of the Indonesian Tropical Animal Agriculture Vol 40, No 3 (2015): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.40.3.145-152

Abstract

Changes of sperm heads morphometric and DNA integrity of freeze-dried bovine spermatozoa were investigated. Freeze-dried spermatozoa had stored in the refrigerator at 4°C for 2 years. Computer-assisted sperm analysis (CASA) was used in this study to identify sperm head morphometry, while for DNA integrity analysis using acridine orange staining. Samples were smeared on glass slides, fixed for 2 h in acetic alcohol and stained with acridine orange solution. After staining, each slide was examined at x400 magnification in a fluorescence microscope with axio vision (Zeiss Company, Germany). Proportion of fluorescence red and green emissions of the sperm head were examined and scored. These results indicated that sperm head had enlarged significantly (P<0.05) after freeze-drying process. However, freeze-dried sperm heads morphometry significantly (P<0.05) decrease after incubation for 3 and 6 hours. Changes of DNA integrity of freeze-dried spermatozoa significantly (P<0.05) decrease after incubation for 6 hours. In the present study concluded that (1) freeze-drying spermatozoa caused sperm head morphometric enlarged, whereas incubation time caused sperm heads decreased, (2) DNA integrity of freeze-dried sperm head is still intact during incubation 3 hours, and decreased DNA integrity occur in incubation for 6 hours.
Detection of F94L mutation of the MSTN gene in four Indonesian local cattle breeds Anwar, S.; Volkandari, S. D.; Wulandari, A. S.; Putra, W. P. B.; Sophian, E.; Said, S.
Journal of the Indonesian Tropical Animal Agriculture Vol 45, No 1 (2020): March
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.45.1.7-14

Abstract

The F94L mutation of the MSTN gene (MSTN-F94L) is considered not to cause disrupted the function of the myostatin gene drastically. Interestingly, this mutation has a very significant effect on muscle mass, carcass, or meat yield and meat quality without any associated severe negative problems. This study aimed to confirm the MSTN-F94L mutation in four local cattle breeds in Indonesia. A total of 518 individuals (140 of Bali, 107 of Sumbawa, 168 of Pasundan, and 103 of Holstein-Friesian (H-F) cattle) were used in this study. Genotype identification was performed by PCR-RFLP method. In the present study showed that the wild-type C allele was fixed (1.000) in Bali, Sumbawa, and HF cattle. However, the wild-type C allele and the mutant A allele were found in Pasundan cattle, even though the frequency of the mutant A allele was very low (0.012). Therefore, in conclusion, the mutation of the MSTN-F94L was detected in Pasundan cattle but no in all three cattle breeds. However, the presence of the mutant A allele in Pasundan cattle allegedly derived from Limousin bulls. The further investigation in other local and exotic breeds and its crossing will answer the status of the MSTN-F94L mutation in local cattle breeds in Indonesia.
Non-genetic factor and genetic parameter analysis for growth traits in Sumba Ongole (SO) cattle Putra, W.P.B.; Agung, P.P.; Said, S.
Journal of the Indonesian Tropical Animal Agriculture Vol 43, No 2 (2018): June
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.43.2.94-106

Abstract

The aim of this study was to evaluate non-genetic factors and genetic parameters of the growth traits in Sumba Ongole (SO) cattle. The growth traits were consisted of birth weight (BW), weaning weight (WW), yearling weight (YW), average daily gain of pre-weaning (ADG1) and post-weaning (ADG2). Data from 143 heads of SO cattle (year 2011 to 2016) which raised at PT KAR were used in this study. Generalized Linear Model (GLM) analysis was performed to evaluate non-genetic effect including sex, year of birth, generation and season. Therefore, to evaluate genetic parameters, the heritability (h2) and genetic correlation (rg ) were performed using Paternal Halfshib Correlation method. The results showed that sex of calf had no significant effect (P>0.05) on BW, WW and YW, but year of birth had significant effect on those traits. The factor of season had significant effect on WW. The estimation of h2 values of growth traits were included high category (h2>0.30) and accurate (h2>SE) on BW (0.66±0.42), WW (0.65±0.44), YW (0.67±0.42), ADG1 (0.68±0.45) and ADG2 (0.70±0.43). The estimation of rg values were included high category (rg>0.50) and accurate (rg>SE) on BW-WW (0.87±0.63); BW-YW (0.95±0.87); ADG1-WW (0.99±0.34); WW-YW (0.98±0.48) and ADG1-YW (0.95±0.51). It was concluded that trait of WW could be used as selection criteria to increase YW trait in SO cattle. 
The polymorphism in g.1256G>A of bovine pituitary specific transcription factor-1 (bPIT-1) gene and its association with body weight of Pasundan cattle Putra, W. P. B.; Agung, P. P.; Said, S.
Journal of the Indonesian Tropical Animal Agriculture Vol 44, No 1 (2019): March
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.44.1.19-27

Abstract

Bovine Pituitary specific transcription factor 1 (bPit-1) is one of amino acid that controling pituitary gland in mammals. The pituitary gland is important for secretion of growth hormone from growth genes. This study was carried out to detect polymorphism in the exon 6 of bPit-1 (g.1256G>A) in Pasundan cattle using PCR-RFLP method and its association with body weight. Total of 69 heads (15 males and 54 females) of Pasundan cattle from breeding station (BPPIBT-SP Ciamis, West Java) were used in this study. Research showed that two genotypes of bPit-1/HinfI gene were identified in this study i.e GG (0.90) and AG (0.10) with allele frequencies of 0.05 (A) and 0.95 (G). The polymorphic informative content (PIC) and number of effective allele (ne) values were 0.09 (low) and 1.11. respectively. The Chi-square (χ2) value in the population studied was 0.20 and in Hardy-Weinberg equilibrium (χ2<5.99). It was concluded that the polymorphism of bPit-1/HinfI in Pasundan cattle included of low category and was not associated with body weight.
Effect of addition an amino acid or its combination with EDTA on DNA integrity and morphometry sperm heads of freeze-dried bovine spermatozoa Said, S.; Maulana, T.; Setiorini, S.; Ibrahim, G.E.; Ramadhan, M.N.; Christopher, E.
Journal of the Indonesian Tropical Animal Agriculture Vol 45, No 3 (2020): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.45.3.189-196

Abstract

The objective of the current study was to investigate the effect of addition an amino acid or its combination with EDTA on DNA integrity and morphometry sperm heads of freeze-dried bovine spermatozoa. Spermatozoa were freeze-dried in medium with the addition of an amino acid glycine, cysteine, glutamine, or its combination with EDTA. Sperm head morphometry was identified at 400X magnification using Axio Vision(Zeiss Company, Germany), while for membrane plasma integrity (MPI) determined by calculation of the percentage of spermatozoa having intact plasma membrane by osmotic resistance test method and DNA integrity analysis using acridine orange staining. Sperm head had declined in size after the freeze-drying process, MPI of FD spermatozoa gradually increased significantly when FD solution was added with an amino acid solution (glycine, cysteine) and its combination with EDTA. DNA integrity of all freeze-dried spermatozoa treatments was remaining intact, no significantly different (P>0.01) among treatments. The present study concluded that the addition of an amino acid (glycine, cysteine) or its combination with EDTA could be reduced morphometric sperm heads and plasma membrane damage of freeze-dried bovine spermatozoa, however, DNA integrity of bovine sperm nucleus remaining intact after the freeze-drying process without addition both amino acids and EDTA. 
Selection of Bali cattle based on birth weight and calving interval records at West Nusa Tenggara Province of Indonesia Said, S.; Putra, W. P. B.; Muzawar, M.; Kantong, S. A.
Journal of the Indonesian Tropical Animal Agriculture Vol 45, No 1 (2020): March
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.45.1.15-27

Abstract

Birth weight and calving interval are included of productivity traits that can be increased by selection program. However, the standard of desirable birth weight in cattle during the selection program is important to prevent dystocia incident risk. This study was aimed to select Bali cattle (Bos javanicus) based on Estimated Breeding Value of birth weight (EBVBW) and Most Probable Producing Ability of birth weight (MPPABW) and calving interval (MPPACI). Total of 758 records data of BW were collected from Lombok and Sumbawa islands, West Nusa Tenggara Province. Research showed the average of BW in Bali calves were 15.69±1.70 kg (Lombok) and 13.49±1.89 (Sumbawa). The average of CI in Bali cows at both islands were about 385 days. In addition, the heritability (h2) values of BW in both islands was about 0.90. The repeatability (r) values of BW in both islands were about 0.30. Meanwhile, the r value of CI in Sumbawa island was 0.39. The highest of EBVBW for sire was +4.25 kg by bull’s ID: 0838 (Sumbawa). Meanwhile, the highest of EBVBW for calves was +6.07 kg by calf’s ID: 0917 (Sumbawa). The highest of MPPABW was +2.67 kg by cow’s ID: 0872 (Sumbawa). The lowest of MPPACI was -25.70 days by cow’s ID: 02076 (Lombok). 
Association of growth hormone (GH) gene polymorphism with growth and carcass in Sumba Ongole (SO) cattle Agung, P. P.; Anwar, S.; Putra, W. P. B.; Zein, M. S. A.; Wulandari, A. S.; Said, S.; Sudiro, A.
Journal of the Indonesian Tropical Animal Agriculture Vol 42, No 3 (2017): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.42.3.153-159

Abstract

A study was conducted to identify the polymorphism in the intron 3 of the Growth Hormone (GH) gene and also to evaluate the association of the GH gene polymorphism with growth parameters and dressing percentage in the Sumba Ongole (SO) cattle. A total of 267 individual DNA samples were used in the Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) analysis. The SO cattle growth parameters data (n=44) including birth weight (BW), weaning weight at 205 days of age (WW205), yearling weight at 365 days of age (YW365) and also dressing percentage (DP) (n=122) were investigated in this study. There were three genotypes (AA, AB, and BB) of the GH gene based on the PCR-RFLP analysis with allele frequency was 0.87 and 0.13 for A allele and B allele respectively. The highest genotype frequency in the SO cattle is AA (0.76) and the lowest is BB (0.02). The Heterozygosity Observed (Ho) value in the SO cattle population is 0.23 and Polymorphism Information Content (PIC) value is 0.20. Therefore, the genetic diversity in the SO cattle based on the GH gene polymorphism is quite low. There is no association (P>0.05) in BW, WW205, YW365, and DP with genotypes of the GH gene. As the result, the GH gene in this study cannot be used as a genetic marker in the SO cattle breeding program.
ESTIMATION OF MOST PROBABLE PRODUCING ABILITY VALUE FOR CALF BIRTH'S PERFORMANCE IN SUMBA ONGOLE COWS Said, S.; Agung, P. P.; Putra, W. P. B.; Anwar, S.; Wulandari, A. S.; Sudiro, A.
Journal of the Indonesian Tropical Animal Agriculture Vol 41, No 2 (2016): June
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.41.2.53-60

Abstract

An estimation of most probable producing ability (MPPA) value based on the calves performance is very important for the cow selection. The birth data record of 48 Sumba Ongole (SO) cows and record data from 52 callf birth’s performance were used to estimate the value of MPPA. High of r value (r > 0.30) was obtained at the body length (BL). Birth weight (BW), withers high (WH) and chest girth (CG) had a moderate value (0.10 < r < 0.30) of repeatability. The MPPA value of the cumulative calf birth’s performance of cows based on the highest birth records was 4.64 (cow number 3770) and the lowest was -4.64 (cow number 2283). Calf number B2076 (cow number 3586) had the highest ratio of WH (115.70); BL (124.26); CG (118.90), while the highest ratio of BW (168.35) was obtained in calf number B2095 (cow number 3731). It was concluded that the BW of birth had a moderate value of r (0.10) and could be used as a selection criteria accurately. 
SELECTION OF SUMBA ONGOLE (SO) CATTLE BASED ON BREEDING VALUE AND PERFORMANCE TEST Said, S.; Agung, P. P.; Putra, W. P. B.; Anwar, S.; Wulandari, A. S.; Sudiro, A.
Journal of the Indonesian Tropical Animal Agriculture Vol 41, No 4 (2016): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.41.4.175-187

Abstract

Selection of livestock was purposed to increase productivity. A performance test was conducted using 25 males and 25 females of Sumba Ongole (SO) cattle. The performance test were conducted in three periods (2014, 2015 and 2016) for about 535 days. Descriptive statistics ws used to analyze data of body weight. Cattle with age between 300 to 600 days were used for evaluation. Result showed that heritability value of yearling weight (YW365) was 0.77+0.68 as a high category. The highest breeding value of YW365 were 66.05 kg (male) and 41.89 kg (female). The average of corrected final weight (CFW) weight were 172.55+34.22 kg (male) and 159.80+37.73 kg (female). The performance test in this study obtained six A class (1 bull and 5 cows) based on the standard minimum of body measurements for SO cattle.
MYOSTATIN GENE ANALYSIS IN THE FIRST GENERATION OF THE BELGIAN BLUE CATTLE IN INDONESIA Agung, P. P.; Said, S.; Sudiro, A.
Journal of the Indonesian Tropical Animal Agriculture Vol 41, No 1 (2016): March
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.41.1.13-20

Abstract

A study was conducted to identify the variations of the myostatin and also to evaluate the existence of genetic marker for “double muscling” (11-bp deletion in the third exon of the myostatin gene) in the first generation of Belgian Blue cattle in Indonesia using the Single Strand Conformation Polymorphism (SSCP) and the sequencing analysis. A total of 8 DNA samples belonged to Karya Anugerah Rumpin (KAR) Farm were used in the Single Strand Conformation Polymorphism (SSCP) and the sequencing analysis. There were 3 allele types of myostatin gene based on the SSCP analysis. The Belgian Blue sire has type A allele. The Simmental, Wagyu, SO x BX, Charolais, and the PO cattle have the type B allele, while the Belgian Blue x FH and the Belgian Blue x SO have the type C allele (heterozygous). There are 11-bp deletion in the third exon myostatin gene for the Belgian Blue sire based on the sequencing analysis. The myostatin gene in the Belgian Blue F1 generation individual was heterozygous. This study provides scientific evidence that the 11-bp deletion in the third exon of myostatin gene in the Belgian Blue sire was inherited to its F1 generation (male and female).
Co-Authors A. S. Wulandari, A. S. A. Sudiro, A. Agung, P.P. Amal, A. S. B. Tappa Baharun, A. C. Arman Christopher, E. F. Afiati, F. I. Arifiantini Ibrahim, G.E. Kantong, S. A. M. A. Setiadi, M. A. M. S. A. Zein, M. S. A. Muzawar, M. N. W. K. Karja, N. W. K. P. P. Agung, P. P. Putra, A.C. R. I. Arifiantini Ramadhan, M.N. S. Anwar Setiorini, S. Sophian, E. T. Maulana, T. Volkandari, S. D. W. P. B. Putra W.P.B. Putra Wulandari, A.S.