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All Journal BERITA BIOLOGI Teknologi Indonesia
Hani Susanti, Hani
LIPI
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT

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Journal : BERITA BIOLOGI

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ISOLASI GEN SITRAT SINTASE BAKTERI Pseudomonas aerugenosa PS2 DARI RIZOSFER POHON KRUING (Dipterocarpus sp.) UNTUK MODEL KONSTRUKSI METABOLISME SEL MIKROALGA BERKARBOHIDRAT RENDAH Susilaningsih, Dwi; Umoro, Asahedi; Ochieng, Fredrick Onyango; Widyaningrum, Dian Noverita; Susanti, Hani; Susilo, Hadi; Swastika, I Nengah; Widyastuti, Utut
BERITA BIOLOGI Vol 18, No 2 (2019)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4410.754 KB) | DOI: 10.14203/beritabiologi.v18i2.2967

Abstract

Pseudomonas has the potential ability for production of citrate synthase synthesis. Pseudomonas aeruginosa could synthesize the enzyme of citrate synthase which is most likely compatible with microalgae cell. Pseudomonas aerugenosa can be found in the rhizosphere of Kruing (Dipterocarpus sp., Dipterocarpaceae). This bacteria is commonly used in agriculture purposes because it is able to synthesize organic acid such as citric acid. These organic acids are synthesized from a reaction between oxaloacetate and acetyl CoA, catalyzed by citrate synthase (CS) in the tricarboxylic acid cycle (TCA). Rhizosphere as microbial sources was obtained from Kruing (Dipterocarpus sp.), which was collected from ?Carita? Research Forest, Pandeglang, Banten, West Java. Citrate synthase gene-specific primers were designed based on citrate synthase gene sequences as depicted in Genbank. The isolation and amplification showed that citrate synthase can be detected and purified from Pseudomonas aeruginosa target and it consists of 1600 bp and encodes 509 amino acids. Based on BLAST (Basic Local Alignment Search Tool) analysis, CS genes that were successfully isolated had 92 % similarity with Pseudomonas aeruginosa type II citrate synthase. This CS gene is expected to be expressed in microalgae metabolism to divert the metabolism of carbohydrate formation into fatty acids. 
EFIKASI LIMBAH SAGU SEBAGAI SUBSTRAT KAYA NUTRISI UNTUK MIKROALGA ISOLAT LIPI11-2-AL002 Susilaningsih, Dwi; Lestari, Sari; Kusnadi, Kusnadi; Hidayat, Topik; Susanti, Hani
BERITA BIOLOGI Vol 13, No 3 (2014)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v13i3.674

Abstract

Microalgae are photosynthetic microorganisms that have potential to produce some useful chemical substances such as carbohydrates, proteins, and lipids. Microalgae are also known exhibited ability as a bioremediation agent. This report is emphasized on analyzing the growth rate and nutritional content of microalgae including carbohydrate, protein and lipid from selected isolates LIPI11-2-AL002 that treated with sago waste. Microalgae were cultivated into hydrolysed sago ?ampas? at the concentration of 0,50, 500,5000 ppm, and medium AF-6 (as control culture) respectively.Therefore selected microalgae isolate was cultivated in series of cultivation volume start from 100 mL until 5 Liter media gradually. Observed parameters were covered cell viability (growth) and proximate content of biomass including carbohydrate,lipid and protein content. The results showed that the carbohydrate and protein content in the algal biomass was increase along the addition of series sago ?-ampas? concentration.The highest concentration of addition the sago ampas is 5000 ppm which is limit for the algal survival. In the highest treatment of sago waste the alga l proximate contents were 261.09 ppm of carbohydrate, 5.12 ppm of protein and 3.61% per of lipid dry weight respectively. In addition, the toxicity effect of fermentation product was not appeared in a toxicity test using gold fish komet(Carassius auratus).
Co-Authors A.A. Ketut Agung Cahyawan W DWI SUSILANINGSIH Hadi Susilo, Hadi Ochieng, Fredrick Onyango Rahman, Delicia Y. Sari Lestari Swastika, I Nengah Topik Hidayat Umoro, Asahedi UTUT WIDYASTUTI Widyaningrum, Dian Noverita