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All Journal Program Kreativitas Mahasiswa - Penelitian BERITA BIOLOGI
Hadi Susilo, Hadi
Program Studi Peternakan, Fakultas Peternakan, Universitas Brawijaya, Malang
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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FOXID-EGG: PENGEMBANGAN INDUSTRI NUTRACEUTICAL BERBASIS TELUR ITIK LOKAL KAYA OMEGA-3 Andri, Faizal; Sukoco, Aji; Hilman, Taufich; Susilo, Hadi; Sibawi, Sibawi
Program Kreativitas Mahasiswa - Penelitian PKM-P 2014
Publisher : Ditlitabmas, Ditjen DIKTI, Kemdikbud RI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (316.984 KB)

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Cardiovascular disease is the number one cause of death and disability worldwide. The purpose of this experiment was to create local ducks egg enriched with omega-3 as a candidate of nutraceutical to prevent cardiovascular disease. Sixty local ducks were randomly distributed to six treatment with three levels of fish oil (0 ppm, 1500 ppm, and 3000 ppm) and two levels of tomato meal (0 ppm and 150 ppm) in a 3x2 factorial arrangement. Data of local ducks performances were analyzed using two-way Anova, data of egg yolk omega-3 fatty acid content was analyzed descriptively. There was an interaction (p<0.05) of fish oil and tomato meal supplementation on egg weight. Supplementation of 3000 ppm fish oil +150 ppm tomato meal tend to increase DHA content of egg yolk with 0,43% compare than control treatment with 0,18%. The conclusion of this research supplementation of 3000 ppm fish oil +150 ppm tomato meal could produce egg yolk of local ducks egg with 0,04% ALA and 0,43% DHA.Keywords:  cardiovascular disease, DHA, local ducks egg, fish oil, tomato meal
ISOLASI GEN SITRAT SINTASE BAKTERI Pseudomonas aerugenosa PS2 DARI RIZOSFER POHON KRUING (Dipterocarpus sp.) UNTUK MODEL KONSTRUKSI METABOLISME SEL MIKROALGA BERKARBOHIDRAT RENDAH Susilaningsih, Dwi; Umoro, Asahedi; Ochieng, Fredrick Onyango; Widyaningrum, Dian Noverita; Susanti, Hani; Susilo, Hadi; Swastika, I Nengah; Widyastuti, Utut
BERITA BIOLOGI Vol 18, No 2 (2019)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4410.754 KB) | DOI: 10.14203/beritabiologi.v18i2.2967

Abstract

Pseudomonas has the potential ability for production of citrate synthase synthesis. Pseudomonas aeruginosa could synthesize the enzyme of citrate synthase which is most likely compatible with microalgae cell. Pseudomonas aerugenosa can be found in the rhizosphere of Kruing (Dipterocarpus sp., Dipterocarpaceae). This bacteria is commonly used in agriculture purposes because it is able to synthesize organic acid such as citric acid. These organic acids are synthesized from a reaction between oxaloacetate and acetyl CoA, catalyzed by citrate synthase (CS) in the tricarboxylic acid cycle (TCA). Rhizosphere as microbial sources was obtained from Kruing (Dipterocarpus sp.), which was collected from ?Carita? Research Forest, Pandeglang, Banten, West Java. Citrate synthase gene-specific primers were designed based on citrate synthase gene sequences as depicted in Genbank. The isolation and amplification showed that citrate synthase can be detected and purified from Pseudomonas aeruginosa target and it consists of 1600 bp and encodes 509 amino acids. Based on BLAST (Basic Local Alignment Search Tool) analysis, CS genes that were successfully isolated had 92 % similarity with Pseudomonas aeruginosa type II citrate synthase. This CS gene is expected to be expressed in microalgae metabolism to divert the metabolism of carbohydrate formation into fatty acids. 
Co-Authors Aji Sukoco DWI SUSILANINGSIH Faizal Andri Hakim, M Nasrun Hani Susanti, Hani Ochieng, Fredrick Onyango Rahayu, Rt Inu Sibawi Sibawi, Sibawi Swastika, I Nengah Taufich Hilman, Taufich Umoro, Asahedi Usman Setiawan UTUT WIDYASTUTI Widyaningrum, Dian Noverita