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All Journal JURNAL TEKNOLOGI LINGKUNGAN Indonesian Journal of Biotechnology Lembaran Publikasi Minyak dan Gas Bumi Makara Journal of Science
Witono Basuki, Witono
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Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Earth & Planetary Sciences Energy Environmental Science Immunology & microbiology Materials Science & Nanotechnology

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Biodegradation of Used Engine Oil by Acinetobacter junii TBC 1.2 Basuki, Witono; Syahputra, Khairul; Suryani, Ayu Tri; Pradipta, Ilham
Indonesian Journal of Biotechnology Vol 16, No 2 (2011)
Publisher : Universitas Gadjah Mada

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Abstract

AbstractThe isolates have capability to degrade used engine oil was obtained from soil in the beach contaminatedwith used engine oil. One of the selected isolates TBC 1.2 was identified by its 16s rDNA as Acinetobacterjunii. The microorganism can use hydrocarbons in used engine oil as the sole carbon source and energy, alsoit significantly degraded almost all hydrocarbon compounds in used engine oil. With its ability Acinetobacterjunii TBC 1.2 has a potency to be utilized for bioremediation of soil polluted with used engine oil.Keywords : biodegradation, used engine oil, Acenitobacter junii TBC 1.2
BIODEGRADASI LIMBAH OLI BEKAS OLEH Lycinibacillus sphaericus TCP C 2.1 Basuki, Witono
Jurnal Teknologi Lingkungan Vol. 12 No. 2 (2011)
Publisher : Center for Environmental Technology - Agency for Assessment and Application of Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (263.804 KB) | DOI: 10.29122/jtl.v12i2.1243

Abstract

Mikroorganism has capability to degrade used engine oil was isolated from soil sample contaminated with used engine oil. One of the selected strain TCP C 2.1 was identified by 16s rDNA as Lycinibacillus sphaericus. The microorganism can use hydrocarbon in used engine oil as the sole carbon source and energy, also it significantly degraded almost all hydrocarbon compounds in used engine oil. With its ability the microorganism has potency to be used as a single microbe for bioremediation of soil polluted by engine oil.
Kemampuan Air Kelapa sebagai Nutrisi untuk Pertumbuhan Mikroba Minyak Bumi Kadarwati, sri; Basuki, Witono; Rudiyanto, Aris
Lembaran publikasi minyak dan gas bumi Vol 43, No 1 (2009)
Publisher : PPPTMGB "LEMIGAS"

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (48.789 KB) | DOI: 10.29017/LPMGB.43.1.125

Abstract

Pertumbuhan dan aktivitas mikroba di lingkungan minyak bumi dapat berdampak positif yang perlu dimanfaatkan, antara lain dalam pengolahan limbah minyak dan teknologi MEOR (microbial enhanced oil recovery). Metabolit yang dihasilkan tergantung pada komposisi nutrisi, jenis mikroba, dan kondisi lingkungannya. Penelitian ini bertujuan untuk menguji kemampuan air kelapa sebagai nutrisi untuk pertumbuhan mikroba minyak bumi. Percobaan dilakukan pada suhu uji 50oC dan dengan tiga variasi media M1, M2, M3, dan mikroba yang digunakan adalah campuran bakteri hasil isolasi dari Sumur 1. Parameter yang diuji adalah pertumbuhan mikroba, viskositas minyak bumi, tegangan antarmuka medium-minyak, dan derajat keasaman. Hasil percobaan menunjukkan bahwa penurunan tegangan antarmuka terbesar terjadi pada media M3 (85,66%), kemudian M2 (47,63%). Sedangkan untuk viskositas minyak bumi, dengan media M3 naik 23,06% dan media M2 turun 33,14%. Untuk media M1 tidak ada penurunan dari keduanya. Derajat keasaman untuk ketiga media masih dalam batas kisaran pertumbuhan mikroba. Dari hasil ini menunjukkan bahwa media M2 dan M3 mempunyai prospek untuk teknologi MEOR.
Bioconversion of Glycerol to Docosahexaenoic Acid by Thraustochytrium WB-02 an Indigenous Indonesian Microalga Strain Basuki, Witono
Makara Journal of Science
Publisher : UI Scholars Hub

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Abstract

Glycerol is a by-product of the biodiesel industry, and it can be processed to produce many useful derivatives. This study is aimed at examining the bioconversion of glycerol to docosahexaenoic acid (DHA) using local microalgae. Glycerol to docosahexaenoic acid converting microalgae were obtained from the mangrove area in the coastal sea of Lampung Province. The single colony was then generated by the scratching technique in its isolation and purification process. By using 18S rDNA, a potential strain namely WB-02, was identified as Thraustochytrium sp. Gas chromatog-raphy analysis was performed to identify its product conversion. As a result, Thraustochytrium WB-02 was identified toutilize glycerol as a single carbon source and convert to DHA. A maximum DHA yield of more than 3.4 g/L was ob-tained when the glycerol concentration in the medium was 8%. Thraustochytrium WB-02 was regarded as a potential microalgae resource in producing DHA due to its high level of production.
Biodegradation of Used Synthetic Lubricating Oil by Brevundimonas diminuta AKL 1.6 Basuki, Witono
Makara Journal of Science
Publisher : UI Scholars Hub

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Abstract

Illegal disposal of used lubricating oil is a serious hazard to the environment and public health. This study is aimed at examining the biodegradation process of used synthetic lubricant using local microorganism. Oil-degrading microorganism were obtained from sea water contaminated with used lubricating oil. Those that showed some growth activity in Bushnell-Haas media containing used synthetic lubricating oil as a sole carbon source were selected. Its single colony was then generated by performing the dilution and scratch technique in the isolation and purification process. By using 16s rDNA, a potential strain namely AKL 1.6, was identified as Brevundimonas diminuta. Gas chromatography analysis was performed before and after the biodegradation process of the used oil. As a result, B. diminuta AKL 1.6 was found to utilize used synthetic lubricating oil as the sole source of carbon and to degrade most of the hydrocarbon within the oil for 14 days. Thus, this particular microorganism has the potential to be the single microbe for bioremediation of sea water contaminated by lubricating oil.
Co-Authors Ayu Tri Suryani, Ayu Tri Ilham Pradipta, Ilham Khairul Syahputra, Khairul Rudiyanto, Aris Rudiyanto, Aris Sri Kadarwati