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All Journal Jurnal AgroBiogen Indonesian Journal of Biotechnology Elkawnie 3BIO: Journal of Biological Science, Technology and Management Prosiding Seminar Nasional Biologi, Teknologi dan Kependidikan (Biotik)
Erly Marwani, Erly
Sekolah Ilmu dan Teknologi Hayati, Institut Teknologi Bandung, Jl. Ganesa 10, Bandung
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Dentistry Earth & Planetary Sciences Education Energy Engineering Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Library & Information Science Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Other

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Agrobacterium-Mediated Stable Transformation of Medicinal Plant Andrographis paniculata Callus Expressing β-glucuronidase (GUS) Gene Marwani, Erly; Tangapo, Agustina; Dwivany, Fenny Martha
Indonesian Journal of Biotechnology Vol 18, No 2 (2013)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (406.629 KB)

Abstract

This study was carried out to establish a stable genetic transformation in callus culture of Andrographispaniculata mediated by Agrobacterium tumefaciens. The leaf disks of A. paniculata were infected with A. tumefaciensLBA4404 carrying a binary vector pCAMBIA1304 that contain β-glucuronidase (GUS) and hygromycinphosphotransferase (hpt) genes. The infection was conducted by dipping method for one hour, followed byco-cultivation in the dark for three days. To examine transient GUS expression, the co-cultivated leaf disks wereassayed for β-glucuronidase activity and to obtain stable transformed callus, the co-cultivated leaf disks wereselected on the callus induction medium which contain 20 mg/l hygromycin for selection. The transformedcallus was periodically subcultured every three weeks into the fresh selection medium over the 15 weeksperiod. To test a stable transformation, the callus was subjected to PCR analysis for GUS gene detection. Theresults indicated that the co-cultivated leaf disks expressed GUS activity and proliferated to produce callus onthe selective medium. Analysis of PCR on the transformed callus indicated the presence 976 bp fragment thatconfi rmed the presence of β-glucuronidase gene. These fi ndings imply that the β-glucuronidase was stablyintegrated into A. paniculata callus culture.Keywords: Andrographis paniculata, Agrobacterium tumefaciens, andrographollide, transformed callus,β-glucuronidase gene.
Pengaruh Cekaman Aluminium terhadap Kandungan Asam Organik dalam Kalus dan Pinak Tomat (Lycopersicon esculentum Mill.) Wening Enggarini; Erly Marwani
Jurnal AgroBiogen Vol 2, No 1 (2006): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v2n1.2006.p24-29

Abstract

The purpose of this research was to evaluate the effects of Al stress on citric, malic and oxalic acid content of L. esculentum cv. Intan callus and plantlet, also aluminum content of L. esculentum plantlet. Callus was induced from cotyledone of L. esculentum on Murashige & Skoog (MS) media containing 10-7 M NAA and 10-6 kinetin. The callus was then transferred step wisely at 3 weeks interval to media containing 220, 275, 330, 385, 440, 550, 825, and 1100 μM AlCl3. The callus cultures on the control media and media with the addition of 550 μM AlCl3 were able to regenerate and produce shoots after 8 passages of subculture. The shoots from media with the addition of 550 μM AlCl3 were transferred into the media with addition of 825 μM AlCl3, then to the media with 1100 μM AlCl3. The High Pressure Liquid Chromatography (HPLC) analysis showed that Al stress callus and plantlets contained malic acid, but no citric and oxalic acid. The content of malic acid in callus decreased with increasing AlCl3 concentration from 0 to 385 μM. On the other hand, the content of malic acid in callus increased with increasing AlCl3 concentration from 440 μM to 1100 μM. Similarly, the content of malic acid in root increased with increasing concentration of AlCl3 from 550 μM to 1100 μM. The result of Neutron Activation Analysis showed that Al content in root decreased as the amount of AlCl3 increased in the media. These results suggested that L. esculentum callus and plantlet respond to the Al stress by producing higher amount of malic acid.
Analisis Ekstrak Etanol Tangkai Daun Buasbuas (Premna pubescens) Menggunakan Gas Chromatography Mass Spectrophotometer (GCMS) Diky Setya Diningrat; Martina Restuati; Kusdianti Kusdianti; Ayu Nirmala Sari; Erly Marwani
Elkawnie: Journal of Islamic Science and Technology Vol 4, No 1 (2018)
Publisher : Universitas Islam Negeri Ar-Raniry Banda Aceh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/ekw.v4i1.3075

Abstract

Buasbuas (Premna pubescens) is a plant that is traditionally known to have medicinal properties. This study aims to determine the content of bioactive compounds contained in the petiole by Gas Chromatography Mass Spectrophotometer (GCMS) method. Preparation of ethanol extract of petiole using maceration method with 96% ethanol solvent. The study used gas chromatographic tools and mass spectra which were evaluated using MASSLAB program. The data obtained from the GCMS machine is then analyzed using the NCBI database pubchem software (https://pubchem.ncbi.nlm.nih.gov/). The results of this study indicate that the content of bioactive compounds on the petioles of buasbuas more than 50 libraries contains about 150 species of compounds with a range of RT and% area respectively 4.684 to 28.155 and 0.16 to 15.56%. The content of bioactive compounds shown this data indicates that very large potential of buasbuas plants to be explored and exploitation as a nutritious plant. The results of this study can be used as the foundation in the development program of the potential utilization of bioassemblance of buasbuas plants. In further research it is necessary to analyze the other parts of the plant and make comparisons to complete the available databases.
Correlation of Microclimate of West Java on Caffeine and Chlorogenic acid in Coffea canephora var. robusta Suci Awaliyah; Sri Nanan B. Widiyanto; Rijanti R. Maulani; Asep Hidayat; Ujang Dinar Husyari; Tati Suryati Syamsudin; Erly Marwani
3BIO: Journal of Biological Science, Technology and Management Vol. 4 No. 1 (2022)
Publisher : School of Life Sciences and Technology, Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/3bio.2022.4.1.7

Abstract

Caffeine and chlorogenic acid (CGA) are two compounds that play a role in determining the quality of coffee. The amount of the two compounds may vary depending on the environment where they are grown. This study aimed to determine the correlation between the local microclimatic condition and the concentration of caffeine and CGA in green and roasted beans of Robusta coffee from six different cultivation areas in West Java, Indonesia (i.e., Ciamis, Tasikmalaya, Sumedang, Kuningan, Cianjur, and Bogor). Samples of green beans and roasted beans were extracted with 70% methanol for caffeine analysis and ethyl acetate for CGA analysis. Caffeine and CGA were analyzed by UV-HPLC using a C18 shimpack gist shimadzu column, with an isocratic elution of methanol:water (1:1) at a 1 mL/min flow rate. Detection was performed at  λ272 nm and λ324 nm for caffeine and chlorogenic acid, respectively. Principal component analysis (PCA) was used to evaluate the correlation between microclimate with caffeine and chlorogenic acid. Results indicated that the concentration of caffeine ranged from 7.67 to 16.52% and 10.79 to 15.56% in the green and roasted bean coffee, respectively. The concentration of CGA ranged from 0.74 to 3.03% and 0.25 to 0.77% in the green and roasted bean coffee, respectively. Based on PCA analysis, the most influential microclimate on the caffeine concentration were the humidity, temperature, and altitude, with the total variance of PC1 and PC2 of  76.3%. However, there was no positive correlation between the measured microclimate and the CGA concentration. In conclusion, Robusta coffee's caffeine content is positively affected by the microclimatic condition (i.e., humidity, temperature, and altitude).
ISOLASI SENYAWA BIOINSEKTISIDA PADA EKSTRAK ETANOL TUMBUHAN BUASBUAS (Premna pubescens Blume) DENGAN METODE GCMS Diky Setya Diningrat; Sonya Adelina Sipayung; Martina Restuati; Erly Marwani; Ayu Nirmala Sari; Kusdianti Kusdianti
Prosiding Seminar Nasional Biotik Vol 6, No 1 (2018): PROSIDING SEMINAR NASIONAL BIOTIK VI 2018
Publisher : Prosiding Seminar Nasional Biotik

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (386.492 KB) | DOI: 10.3126/pbio.v6i1.4312

Abstract

Penelitian ini bertujuan untuk mengetahui pada bagian tumbuhan buasbuas (Premna pubescens Blume) manakah senyawa insektisida terakumulasi dan membandingkan jenis senyawa insektisida yang terdapat di daun muda, daun tua, tangkai daun, dan buah dengan teknik Gas Chromatography – Mass Spectrometry (GC-MS). Ekstrak buasbuas dibuat menggunakan metode maserasi dengan pelarut etanol 96% dan di angin-anginkan hingga menjadi pasta. Pasta yang didapatkan kemudian dianalisis dengan GC-MS. Data yang diperoleh dari hasil GC-MS yang diidentifikasi dengan software PubChem (https://pubchem.ncbi.nlm.nih.gov). Hasil penelitian menunjukkan adanya senyawa 7-methyl-1-benzofuran yang merupakan insektisida terakumulasi pada daun muda sebanyak 0,011% dari total 87 senyawa bioaktif. Pada bagian tumbuhan buasbuas lainya tidak ditemukan senyawa insektisida. Pada daun muda dan daun tua ditemukan herbisida yaitu kelompok senyawa yang sama dengan insektisida tergolong kedalam senyawa “cydal”. Kesimpulan penelitian ini adalah senyawa insektisida terdapat pada tanaman buasbuas, meskipun hanya satu jenis senyawa insektisida yang ditemukan dari keseluruhan senyawa bioaktif tanaman buasbuas yang diidentifikasi.
Co-Authors Asep Hidayat Ayu Nirmala Sari Diningrat, Diky Setya FENNY MARTHA DWIVANY Kusdianti Kusdianti Kusdianti Kusdianti Restuati, Martina Rijanti R. Maulani Sonya Adelina Sipayung Sri Nanan B. Widiyanto Suci Awaliyah Tangapo, Agustina Monalisa Tati Suryati Syamsudin Ujang Dinar Husyari Wening Enggarini