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All Journal The Journal of Experimental Life Sciences (JELS) Biotropika
Nia Kurniawan
University of Brawijaya
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Neuroscience

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Anuran diversity and community structure in Lesti upriver across buffer zone habitat in Bromo Tengger Semeru National Park Muhammad Fathoni; Luchman Hakim; Nia Kurniawan
Biotropika: Journal of Tropical Biology Vol. 10 No. 1 (2022)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2022.010.01.10

Abstract

In Bromo Tengger Semeru National Park (BTSNP), buffer zone areas, one of which is the Lesti upstream rivers, play a critical role in sustaining biodiversity and community life. Frogs serve as a bioindicator of environmental change, particularly in the upstream rivers of the Lesti. The present study examines the diversity and community structure of Anura in the upstream rivers of Lesti, which are included in the BTSNP buffer zones. Throughout the surveys, up to 14 anuran species were encountered in four sites adjacent to the BTSNP border and crossed by the Lesti rivers. This study suggests that buffer zones may support herpetofaunal diversity by allowing humans to interact with them and sustainably utilize the resources in the areas. Urban development and conservation in these areas must be kept current in order to minimize environmental disruption caused by humans.
Phylogenetics and Biogeography of Cobra (Squamata: Naja) in Java, Sumatra, and Other Asian Region Nia Kurniawan; Mulyadiane M. Putri; Ahmad M. Kadafi; Dea J. Chrestella; Muhammad A. Fauzi; Agung S. Kurnianto
The Journal of Experimental Life Science Vol. 7 No. 2 (2017)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1441.886 KB) | DOI: 10.21776/ub.jels.2017.007.02.06

Abstract

The separation of Sumatra and Java occurred at the end of the Miocene (10 mya) to the end of Pliocene (1.8 mya). The existence of ecological variations and geographic barriers inhibits gene flow through the isolation of adaptation, geography, reproduction, inbreeding, and leading to population segregation. Cobra (genus Naja) distribution became greatly influenced by the geologic condition and sea level. This study was conducted by phylogenetic analysis towards the 16S rRNA gene. Survey was done with Visual Encounter Survey (VES) method on 6 localities. There were 3 locations in Sumatra Island and the others in Java Island. Sample from other Asian region was obtained from Genbank, which are 11 individuals from China, Thailand, and Nepal. DNA extraction was done according to the QIAmp® DNA Mini Kit standard protocol. The forward and reverse 16S sequences are combined with the SequencherTM version 4.1.4 program, then in BLAST (Blast Local Alignment Search Tool) at www.ncbi.nlm.nih.gov.  Phylogenetic analyzes of clade A (MP = 60, ML = 54, BI = 88) indicate the presence of division into two monophyletic subclade (AI and AII). Subclade AI consists of groups of Cobra  from Sunda (Thailand, Sumatra and Java). Subclade AII is a group of species N. kaouthia originating from Chumpon Province, Southern Thailand with (MP = 96, ML = 95, BI = 100). Clade B is divided into two subclasses (BI and BII). The result is supported by bootstrap value MP = 93, ML = 99, BI = 100. N. atra of Fujian Province is a sister lineage of the same species from Jiangxi Province (MP = 86, ML = 86, BI = 100).Keywords: Asian, 16s rRNA, Java, Cobra, Sumatra.
Co-Authors Agung S. Kurnianto Ahmad M. Kadafi Dea J. Chrestella Luchman Hakim Muhammad A. Fauzi Muhammad Fathoni Mulyadiane M. Putri