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All Journal JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis)
Andi Mutmainna
Department of Animal Science, Faculty of Science and Technology, Universitas Islam Negeri Alauddin Makassar, Jl. Sultan Alauddin 63, Makassar, South Sulawesi, 90221, Indonesia
Veterinary

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Journal : JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis)

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Antimicrobial activity of plantaricin IIA-1A5 on whey growth medium againts Pseudomonas aeroginosa: a scanning electron microscopy (SEM) study Andi Mutmainna; Irma Isnafia Arief; Ayu Lestari; Muhammad Nur Hidayat
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 9, No 2 (2022): JITRO, May 2022
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (464.24 KB) | DOI: 10.33772/jitro.v9i2.22444

Abstract

The study aimed to understand antimicrobial properties of plantaricin derived from L. plantarum IIA-1A5 and its inhibitory mechanism against Pseudomonas aeruginosa ATCC 27853 under SEM (scanning electron microscopy) observation. The inhibition of microbial growth was evaluated according to diameter of inhibitory zone. Scanning electron microscopy was then applied to observe the microstructure. The diameter of antimicrobial activity against Pseudomonas aeruginosa ATCC 27853 reached 6.52 mm in whey medium and 8.45 mm in whey+ medium (added with sucrose, tryptone and yeast extract). The experimental result clearly demonstrated the inhibition Pseudomonas aeruginosa ATCC 27853. Observation by SEM suggested that the antimicrobial activity of Pseudomonas aeruginosa ATCC 27853 related destruction of cellular morphology. The cell membrane was destroyed as indicated by the release of nucleic acid and reduction of membrane potential. In short, our experiment provided meaningful evidence on the future use of plantaricin IIA-1A5 as food preservative through disruption of cellular membrane causing leading to cell dying.Keywords:  Plantaricin, Lactobacillus plantarun, Pseudomonas aeruginosa, antimicrobial activity
Co-Authors Ayu Lestari Irma Isnafia Arief Muhammad Nur Hidayat