Article Per Year (5 Year)
p-Index From 2019 - 2024
0.408
P-Index
This Author published in this journals
All Journal Indonesian Biodiversity Journal Indonesian Biodiversity Journal
Yermia Semuel Mokosuli
Department of Biology, Faculty of Mathematics and Natural Science, State University of Manado, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Public Health Veterinary

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
Nutmeg (Myristica fragrans) Of North Minahasa Molecular Entication Based on chloroplast DNA of matK gene Fanny Nella Nanlohy; Yermia Semuel Mokosuli
Indonesian Biodiversity Journal Vol. 1 No. 1 (2020): April 2020
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Plant Nutmeg (Myristica fragrans) is an endemic plant in Indonesia originating from Banda Maluku Province. Nutmegs have long been known to people as spices. The nutmeg plants have been cultivated in North Sulawesi for a long time. This research has been conducted to obtain the position of species and the reconstruction of the phylogenetic tree of nutmeg plant (Myristica fragrans) from North Minahasa based on chloroplast DNA of matK gene. This research uses a descriptive method with a laboratory experiment. Total DNA was obtained by leaf tissue extraction and purification. The matK gene was amplified using the PCR method and visualized with automatic electrophoresis, sequencing was performed at FIRST BASE Singapore. The result of the analysis of the gene sequence of North Min Minerals using Barcode Of Life Database (BOLD) Systems shows that Pala Wori and Pala Patokaan have 99% similarity with Myristica maingayi and Myristica glubosa. While the reconstruction of the phylogeny tree shows the North Minahasa Pala has the closest resemblance to Myristica fatua.
Nutmeg (Myristica fragrans) Of North Minahasa Molecular Entication Based on chloroplast DNA of matK gene Fanny Nella Nanlohy; Yermia Semuel Mokosuli
Indonesian Biodiversity Journal Vol. 1 No. 1 (2020): April 2020
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v1i1.245

Abstract

Plant Nutmeg (Myristica fragrans) is an endemic plant in Indonesia originating from Banda Maluku Province. Nutmegs have long been known to people as spices. The nutmeg plants have been cultivated in North Sulawesi for a long time. This research has been conducted to obtain the position of species and the reconstruction of the phylogenetic tree of nutmeg plant (Myristica fragrans) from North Minahasa based on chloroplast DNA of matK gene. This research uses a descriptive method with a laboratory experiment. Total DNA was obtained by leaf tissue extraction and purification. The matK gene was amplified using the PCR method and visualized with automatic electrophoresis, sequencing was performed at FIRST BASE Singapore. The result of the analysis of the gene sequence of North Min Minerals using Barcode Of Life Database (BOLD) Systems shows that Pala Wori and Pala Patokaan have 99% similarity with Myristica maingayi and Myristica glubosa. While the reconstruction of the phylogeny tree shows the North Minahasa Pala has the closest resemblance to Myristica fatua.
Co-Authors Fanny Nella Nanlohy