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Herry Sumampouw
Biology Department, Faculty of Mathematics and Natural Sciences, Manado State University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Public Health Veterinary

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THE BIOACTIVE CONTENT AND ANTIOXIDANT ACTIVITY OF HONEY BEEHIVES (Apis dorsata BINGHAMI) FROM THE BOGANI NANI WARTABONE BOLAANG MONGONDOW NATIONAL PLANT Yermia S Mokosuli; Herry Sumampouw; Hendra Pratama Maliangkay; Jimmy Langi
Indonesian Biodiversity Journal Vol. 2 No. 1 (2021): April 2021
Publisher : Universitas Negeri Manado

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Abstract

Bee Honey (Apis dorsata Binghami) Is a subspecies of A. dorsata which is only found in sulawesidan island and surrounding islands and until now has not been successfully cultivated. Various studies have been conducted species of trees used for nesting places, nesting behavior, biotic conditions, physical surrounding nesting trees and nesting characteristics. This study aims to obtain secondary metabolite components and scientific data of antioxidant activity of honeybee extract (Apis dorsata Binghami) derived from Bogani Nani Wartabone Bolaang Mongondow National Park, North Sulawesi Province. The method of extraction in this research was conducted using maceration with methanol solvent, phytochemical test and antioxidant activity analysis using method (1,1-definil-2-picrilhidrazil) by measuring absorbance absorption at maximum wavelength using UV-Vis spectrophotometer. The results of the phytochemical test show that the honeycomb (Apis dorsata Binghami) contains alkaloids, flavonoids, saponins and tannins. From the analysis results showed that with the increasing concentration of extracts, the greater the antioxidant activity is marked by decreasing the value of IC50. The honeycomb (Apis dorsata Binghami) has IC50 of 212.42 ppm in comparison with vitamin C IC50 of 59.34 ppm
ANTIBACTERIAL ACTIVITY OF CHLOROFORM EXTRACT AND RHIZOPHORA SPP LEAVES METHANOL EXTRACT ON MOUTH BACTERIA Carolin Manuahe; Herry Sumampouw; Friska Montolalu
Indonesian Biodiversity Journal Vol. 2 No. 3 (2021): Desember 2021
Publisher : Universitas Negeri Manado

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The purpose of this study was to obtain the phytochemical profile of the methanol and chloroform extracts of the leaves of Rhizophora apiculata and to find the antibacterial activity of the methanol and chloroform extracts against human oral bacteria. This research was carried out in 3 stages, namely Rhizopora spp. Leaf Sampling, Extraction and Analysis of Secondary Metabolite Content and Antibacterial Activity Test using the agar diffusion method. Based on the results of the phytochemical screening examination of the leaf extract of Rhizopora spp., the results showed that it was rich in chemical compounds of alkaloids, flavonoids, tannins, and saponins in both methanol extract and chloroform extract. The results of the antibacterial activity test of methanol extract obtained that the smallest concentration in bacterial isolate bacteria 2 was 30 mg/ml, while the smallest concentration in bacterial isolate 1 was 50 mg/ml. The results of the antibacterial activity test of the chloroform extract obtained that the smallest concentration in the bacterial isolate bacteria 2 was 20 mg/ml while the smallest concentration in the bacterial isolate 1 was 30 mg/ml. Both methanol extract and chloroform extract had better activity on gram-positive bacteria.
THE BIOACTIVE CONTENT AND ANTIOXIDANT ACTIVITY OF HONEY BEEHIVES (Apis dorsata BINGHAMI) FROM THE BOGANI NANI WARTABONE BOLAANG MONGONDOW NATIONAL PLANT Yermia S Mokosuli; Herry Sumampouw; Hendra Pratama Maliangkay; Jimmy Langi
Indonesian Biodiversity Journal Vol. 2 No. 1 (2021): April 2021
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v2i1.4930

Abstract

Bee Honey (Apis dorsata Binghami) Is a subspecies of A. dorsata which is only found in sulawesidan island and surrounding islands and until now has not been successfully cultivated. Various studies have been conducted species of trees used for nesting places, nesting behavior, biotic conditions, physical surrounding nesting trees and nesting characteristics. This study aims to obtain secondary metabolite components and scientific data of antioxidant activity of honeybee extract (Apis dorsata Binghami) derived from Bogani Nani Wartabone Bolaang Mongondow National Park, North Sulawesi Province. The method of extraction in this research was conducted using maceration with methanol solvent, phytochemical test and antioxidant activity analysis using method (1,1-definil-2-picrilhidrazil) by measuring absorbance absorption at maximum wavelength using UV-Vis spectrophotometer. The results of the phytochemical test show that the honeycomb (Apis dorsata Binghami) contains alkaloids, flavonoids, saponins and tannins. From the analysis results showed that with the increasing concentration of extracts, the greater the antioxidant activity is marked by decreasing the value of IC50. The honeycomb (Apis dorsata Binghami) has IC50 of 212.42 ppm in comparison with vitamin C IC50 of 59.34 ppm
ANTIBACTERIAL ACTIVITY OF CHLOROFORM EXTRACT AND RHIZOPHORA SPP LEAVES METHANOL EXTRACT ON MOUTH BACTERIA Carolin Manuahe; Herry Sumampouw; Friska Montolalu
Indonesian Biodiversity Journal Vol. 2 No. 3 (2021): Desember 2021
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v2i3.4987

Abstract

The purpose of this study was to obtain the phytochemical profile of the methanol and chloroform extracts of the leaves of Rhizophora apiculata and to find the antibacterial activity of the methanol and chloroform extracts against human oral bacteria. This research was carried out in 3 stages, namely Rhizopora spp. Leaf Sampling, Extraction and Analysis of Secondary Metabolite Content and Antibacterial Activity Test using the agar diffusion method. Based on the results of the phytochemical screening examination of the leaf extract of Rhizopora spp., the results showed that it was rich in chemical compounds of alkaloids, flavonoids, tannins, and saponins in both methanol extract and chloroform extract. The results of the antibacterial activity test of methanol extract obtained that the smallest concentration in bacterial isolate bacteria 2 was 30 mg/ml, while the smallest concentration in bacterial isolate 1 was 50 mg/ml. The results of the antibacterial activity test of the chloroform extract obtained that the smallest concentration in the bacterial isolate bacteria 2 was 20 mg/ml while the smallest concentration in the bacterial isolate 1 was 30 mg/ml. Both methanol extract and chloroform extract had better activity on gram-positive bacteria.
Co-Authors Carolin Manuahe Friska Montolalu Hendra Pratama Maliangkay Jimmy Langi Yermia S Mokosuli