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Masje Wurarah
Biology Department, Faculty of Mathematics and Natural Sciences, Manado State University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Public Health Veterinary

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ANTIBACTERIAL ACTIVITY OF EXTRACTS OF LEAVES OF LEILEM (Clerodendrum minahassae Teijsm. & Binn.) ON GROWTH GRAM - POSITIVE BACTERIA Staphylococcus aureus strains ATCC 25923 Jefry Oldy Raturandang; Dintje Pendong; Masje Wurarah
Indonesian Biodiversity Journal Vol. 2 No. 1 (2021): April 2021
Publisher : Universitas Negeri Manado

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Abstract

Leaf leilem (Clerodendrum minahassae Teism. & Binn.) commonly used by the Minahasa as food and traditional medicine for treating intestinal worms, abdominal pain, and pain in the chest. Gram-positive bacteria Staphylococcus aureus ATCC 25923 strain is a type of bacteria that is found in humans and can be the cause of the emergence of the disease. This research aims to know the types of solvents are most effective and how the antibacterial activity of extracts of leaves of leilem on the growth of the bacteria Staphylococcus aureus strains ATCC 25923. The solvent used is ethanol is polar, ethyl acetate which is semi polar, and n-heksan which is non polar. Preliminary research has been conducted on konsenterasi extract with 100 ppm 200 ppm 400 ppm and 800 ppm, the antibacterial activity of the most optimal at 800 ppm so the data will be used for the analysis in this study was konsenterasi 800 ppm. Deuteronomy was done by as much as 3 times. Positive control using antibiotics clindamicyn 400 ppm and negative controls using aquades and 10% DMSO. Isolation of bacteria using scratch in zig-zag and to test the antibacterial activity of using diffusion well in order. Results of the study showed that the percentage yield of solvent is ethanol that is 1,2329% in maceration 1:4. Drag the diameter of most bacteria are grown on ethyl acetate extract polar spring with a grade average of 12.6 mm. Based on the analysis of OneWay ANOVA showed different results for real then followed by Tukey test, to see that these three types of different extract significantly in inhibiting the growth of gram positive bacteria Staphylococcus aureus strains ATCC 25923. In conclusion, namely the first ethanol is a solvent that is most effective in dissolving the leaves leilem, both of the antibacterial activity in sequence from the most minor to the most massive is the n-heksan (non-polar), ethanol (polar), and ethyl acetate (semi polar). The third conclusion in the optimum konsenterasi 800 ppm, extract n-heksan n-heksan (non-polar), ethanol (polar), and ethyl acetate (semi polar) showed antibacterial activity significantly different.
POTENTIAL ANTIBACTERIAL EXTRACTS OF MANGROVE GENUS AVICENIA ROOT AND LEAVES FROM KOMBI BEACH MINAHASA Masje Wurarah; Devitha Kalitouw; Raflen Aril Gerungan
Indonesian Biodiversity Journal Vol. 2 No. 3 (2021): Desember 2021
Publisher : Universitas Negeri Manado

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Abstract

This study aimed to obtain the phytochemical content of the crude extract of leaves and roots of Avicenia spp from Kombi Beach Minahasa. Obtaining data on the activity of inhibiting the growth of infective microbes on external wounds of crude extract of leaves and roots of Avicenia spp from Kombi Beach, Minahasa. To determine the concentration of minimum growth inhibition of crude extract of leaves and roots of Avicenia spp from Kombi Beach, Minahasa. Knowing the crude extract (crude extract) leaves and roots of Avicenia spp originating from the Minahasa Kombi Beach is bactericidal or bacteriostatic. This research consisted of extraction, phytochemical analysis and antibacterial activity test. The results showed that the dominant secondary metabolites in the root and leaf extracts were alkaloids, flavonoids, saponins and tannins. The chloroform extract inhibited the growth of bacteria in infected wounds more than the ethanol and n-hexane extracts, at concentrations of 15 and 30 ppm for the test solution of ethanol extract with an inhibitory diameter of < 7 mm and a concentration of 50 ppm with an inhibitory diameter of > 10 mm indicating that the high bioactive activity antibacterial.
ANTIBACTERIAL ACTIVITY OF EXTRACTS OF LEAVES OF LEILEM (Clerodendrum minahassae Teijsm. & Binn.) ON GROWTH GRAM - POSITIVE BACTERIA Staphylococcus aureus strains ATCC 25923 Jefry Oldy Raturandang; Dintje Pendong; Masje Wurarah
Indonesian Biodiversity Journal Vol. 2 No. 1 (2021): April 2021
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v2i1.4931

Abstract

Leaf leilem (Clerodendrum minahassae Teism. & Binn.) commonly used by the Minahasa as food and traditional medicine for treating intestinal worms, abdominal pain, and pain in the chest. Gram-positive bacteria Staphylococcus aureus ATCC 25923 strain is a type of bacteria that is found in humans and can be the cause of the emergence of the disease. This research aims to know the types of solvents are most effective and how the antibacterial activity of extracts of leaves of leilem on the growth of the bacteria Staphylococcus aureus strains ATCC 25923. The solvent used is ethanol is polar, ethyl acetate which is semi polar, and n-heksan which is non polar. Preliminary research has been conducted on konsenterasi extract with 100 ppm 200 ppm 400 ppm and 800 ppm, the antibacterial activity of the most optimal at 800 ppm so the data will be used for the analysis in this study was konsenterasi 800 ppm. Deuteronomy was done by as much as 3 times. Positive control using antibiotics clindamicyn 400 ppm and negative controls using aquades and 10% DMSO. Isolation of bacteria using scratch in zig-zag and to test the antibacterial activity of using diffusion well in order. Results of the study showed that the percentage yield of solvent is ethanol that is 1,2329% in maceration 1:4. Drag the diameter of most bacteria are grown on ethyl acetate extract polar spring with a grade average of 12.6 mm. Based on the analysis of OneWay ANOVA showed different results for real then followed by Tukey test, to see that these three types of different extract significantly in inhibiting the growth of gram positive bacteria Staphylococcus aureus strains ATCC 25923. In conclusion, namely the first ethanol is a solvent that is most effective in dissolving the leaves leilem, both of the antibacterial activity in sequence from the most minor to the most massive is the n-heksan (non-polar), ethanol (polar), and ethyl acetate (semi polar). The third conclusion in the optimum konsenterasi 800 ppm, extract n-heksan n-heksan (non-polar), ethanol (polar), and ethyl acetate (semi polar) showed antibacterial activity significantly different.
POTENTIAL ANTIBACTERIAL EXTRACTS OF MANGROVE GENUS AVICENIA ROOT AND LEAVES FROM KOMBI BEACH MINAHASA Masje Wurarah; Devitha Kalitouw; Raflen Aril Gerungan
Indonesian Biodiversity Journal Vol. 2 No. 3 (2021): Desember 2021
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v2i3.4985

Abstract

This study aimed to obtain the phytochemical content of the crude extract of leaves and roots of Avicenia spp from Kombi Beach Minahasa. Obtaining data on the activity of inhibiting the growth of infective microbes on external wounds of crude extract of leaves and roots of Avicenia spp from Kombi Beach, Minahasa. To determine the concentration of minimum growth inhibition of crude extract of leaves and roots of Avicenia spp from Kombi Beach, Minahasa. Knowing the crude extract (crude extract) leaves and roots of Avicenia spp originating from the Minahasa Kombi Beach is bactericidal or bacteriostatic. This research consisted of extraction, phytochemical analysis and antibacterial activity test. The results showed that the dominant secondary metabolites in the root and leaf extracts were alkaloids, flavonoids, saponins and tannins. The chloroform extract inhibited the growth of bacteria in infected wounds more than the ethanol and n-hexane extracts, at concentrations of 15 and 30 ppm for the test solution of ethanol extract with an inhibitory diameter of < 7 mm and a concentration of 50 ppm with an inhibitory diameter of > 10 mm indicating that the high bioactive activity antibacterial.
Co-Authors Devitha Kalitouw Dintje Pendong Jefry Oldy Raturandang Raflen Aril Gerungan