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All Journal VIGOR: JURNAL ILMU PERTANIAN TROPIKA DAN SUBTROPIKA
Effi Alfiani Sidik
Loka Penelitian Penyakit Tungro
Agriculture, Biological Sciences & Forestry

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IDENTIFIKASI CENDAWAN TERBAWA BENIH PADI MENGGUNAKAN BLOTTER TEST DAN PREPARASI METODE SELOTIP Effi Alfiani Sidik
JURNAL VIGOR Vol 6, No 2 (2021): VIGOR: Jurnal Ilmu Pertanian Tropika dan Subtropika
Publisher : Universitas Tidar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (487.795 KB) | DOI: 10.31002/vigor.v6i2.4482

Abstract

Seed health testing can help to limit the danger of seed-borne diseases, particularly fungus, spreading from one location to another. To detect several types of seed-borne fungus, seed testing utilizing the filter paper incubation method (blotter test) was carried out. Under a microscope, the isolates acquired after incubation were identified. The creation of sample preparations becomes a crucial key in facilitating the easy and practical identification of fungus. The purpose of this study was to use a blotter test to identify rice seed-borne fungus using the tape method to prepare sample. Incubation was accomplished by planting the seeds on Whatman paper and exposing them to 12 hours of light and 12 hours of darkness alternately. The tape method was used for the preparation of the fungus that grows on the seeds. The tape method is a quick, simple, and practical way to detect the fungus that covers the seed's surface. Tilletia barclayana, Phoma sp., Fusarium sp., Aspergillus niger, Desclera oryzae, Curvularia lunata, C. pallescens, Stemphylium sp. and Nigrospora sp. have all been detected.Keywords: Benih padi, Mikroskop, Morfologi cendawan, Patogen tular benih, Seed-borne
Deteksi Molekuler Asosiasi Begomovirus dengan Penyakit Keriting Kuning Cabai di Pakis dan Banyuurip, Magelang Indonesia Effi Alfiani Sidik
JURNAL VIGOR Vol 6, No 1 (2021): VIGOR : JURNAL ILMU PERTANIAN TROPIKA DAN SUBTROPIKA
Publisher : Universitas Tidar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (604.363 KB) | DOI: 10.31002/vigor.v6i1.3794

Abstract

Begomovirus associated to yellow-curl disease, is an obstacle to the production of chilli in various countries, especially in Indonesia. The characterization of the visual symptoms caused by Begomovirus varies widely. The detection method used must be effective and accurate therefore provide a precise control. This study aims to detect Begomovirus infection using PCR and identify symptoms of yellow-curl disease in Magelang Regency. The research included a sample collection on chilli in Pakis and Banyuurip (Magelang), observing of variation of symptoms of Begomovirus infection, and molecular detection. Primer pairs pAL1v1978/pAR1c715 has successfully detected the association of Begomovirus with yellow-curl disease on chilli at Pakis by symptoms: mosaic, leaf cupping, plant stunting, curling, and malformation. PCR results showed that Begomovirus was not association with yellow-curl disease on chilli at Banyuurip. Molecular evidence for Begomovirus is absolutely necessary because detection based on the visual appearance of symptoms alone is not sufficient to prove infection. 
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