This research aims to investigate the effectiveness of Augmented Reality towards the mastery of digestive system of the fifth grade students in Sragen regency. The method of this research was experimental. The population of this research was all the fifth grade students in Sragen regency – 13.173 students, and of all the population, 135 students were selected as the sample. The technique of determining the sample was stratified random sampling. The treatment was performed using Augmented Reality during 4 weeks. The data were collected through test. The test consisted of 25 multiple choice questions, centering on human digestive system. The data obtained were in the form of students’ score, reflecting their mastery of human digestive system. The data were then analyzed using descriptive and inferential statistics. In using Augmented Reality, the students were able to easily visualize what happened and understand complex concepts, so that it could smooth and improve learning process as well as learning outcomes. The results showed that the effectiveness of Augmented Reality was evident in the mastery of human digestive system of the fifth grade students in Sragen regency.