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All Journal Journal of the Indonesian Tropical Animal Agriculture
F. Afiati, F.
Research Centre for Biotechnology, Indonesian Institute of Sciences, Jl. Raya Bogor Km. 46 Cibinong 16911
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences

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Journal : Journal of the Indonesian Tropical Animal Agriculture

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STUDY ON CHANGES OF SPERM HEAD MORPHOMETRY AND DNA INTEGRITY OF FREEZE-DRIED BOVINE SPERMATOZOA Said, S.; Afiati, F.; Maulana, T.
Journal of the Indonesian Tropical Animal Agriculture Vol 40, No 3 (2015): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.40.3.145-152

Abstract

Changes of sperm heads morphometric and DNA integrity of freeze-dried bovine spermatozoa were investigated. Freeze-dried spermatozoa had stored in the refrigerator at 4°C for 2 years. Computer-assisted sperm analysis (CASA) was used in this study to identify sperm head morphometry, while for DNA integrity analysis using acridine orange staining. Samples were smeared on glass slides, fixed for 2 h in acetic alcohol and stained with acridine orange solution. After staining, each slide was examined at x400 magnification in a fluorescence microscope with axio vision (Zeiss Company, Germany). Proportion of fluorescence red and green emissions of the sperm head were examined and scored. These results indicated that sperm head had enlarged significantly (P<0.05) after freeze-drying process. However, freeze-dried sperm heads morphometry significantly (P<0.05) decrease after incubation for 3 and 6 hours. Changes of DNA integrity of freeze-dried spermatozoa significantly (P<0.05) decrease after incubation for 6 hours. In the present study concluded that (1) freeze-drying spermatozoa caused sperm head morphometric enlarged, whereas incubation time caused sperm heads decreased, (2) DNA integrity of freeze-dried sperm head is still intact during incubation 3 hours, and decreased DNA integrity occur in incubation for 6 hours.
The improvement of functional food in yogurt enriched with purple sweet potato (Ipomea batatas var. Ayamurasaki) Afiati, F.; Priadi, G.; Setiyoningrum, F.
Journal of the Indonesian Tropical Animal Agriculture Vol 43, No 2 (2018): June
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.43.2.159-168

Abstract

he research was conducted to examine the use of purple sweet potato (Ipomoea batatas var. Ayamurasaki) to improve functional food of yogurt. This experiment has 2 factors of treatments. The first factor (i) was concentration of skim milk, i.e. 0%, 3% and 6%; the second factor (ii) was concentration of purple sweet potato, i.e. 0%, 2% and 4% of purple sweet potato. The parameters observed were viability of lactic acid bacteria (LAB), pH, moisture content, protein, fat, carbohydrate, ash and crude fiber and organoleptic. The results showed that there was no interaction between the concentration of skim-milk and the purple sweet potato on total LAB, moisture content and crude fiber. However, the interaction occurred on the levels of fat, protein, carbohydrate and ash. The organoleptic test results that yogurt enriched by 2% purple sweet potato without addition of skim was more preferable by panelist with a value of 3.65.
Co-Authors Priadi, G. S. Said Setiyoningrum, F. T. Maulana, T.