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All Journal Jurnal Ilmiah Perikanan dan Kelautan
Patmawati
Department of Marine, Faculty of Fisheries and Marine, Universitas Airlangga, Surabaya, 60113. Indonesia
Earth & Planetary Sciences

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Effects of Different Acetic Acid Immersion Time on the Properties of Collagen from Pangasius Skin Clara Amelia Kusumawinahyu; Sharilla Aryananti Abidin; Patmawati; Dwi Yuli Pudjiastuti; Dwitha Nirmala; Mochammad Amin Alamsjah; Laksmi Sulmartiwi; Raseetha Vani Siva Manikam
Jurnal Ilmiah Perikanan dan Kelautan Vol. 14 No. 2 (2022): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v14i2.33532

Abstract

Highlight Research Experimental use of pre-treated pangasius skin as collagen raw material using different acetic acid immersion time Effects of immersion time on the yield of collagen from pangasius skin Effects of immersion time on the proximate content of collagen from pangasius skin Presenting SDS-Page profile of collagen from pangasius skin   Abstract Pangasius sp. production in Indonesia has reached 384,310 tons in 2019. One of the main waste in pangasius fillet industry is the skin, which reached to 6% of body mass. Fish skins are alternative for making collagen because it has no restrictions for certain religions and ethnicities. Collagen is a protein biomaterial which acts as the main component of connective tissue. Extracting collagen using organic acids such as acetic acid is the most common extraction method. Herein, we report the effects of different immersion time of acetic acid to collagen properties from pangasius skin. In this study, pangasius skin was treated using 10% alcohol with the ratio 1:10 (w/v) for defatting and 0.1M NaOH with the ratio 1:10 (w/v) for eliminating non-collagenous protein. The immersion of 0.5M acetic acid was carried out on pangasius skin with the ratio of 1:20 (w/v), for 24, 48, and 72h at temperature less than 15oC. The sample was salted-out for 24 hours with NaCl until the concentration reached 2.5M, then the sample was freeze-dried at -40oC. This study investigated that longer immersion time affected the properties of pangasius skin collagen. Different immersion time significantly affected the yield of collagen from pangasius skin (p<0.05). Pangasius skin has a potential to be used as collagen raw material, and 72h of immersion time (P3) is suggested to produce pangasius skin collagen with the highest yield and protein content (6.15 % and 9.26 %). Production of collagen from the fish skin will contribute to increase pangasius waste valorization in pharmaceutical industry.
Effect of Acetic Acid Pretreatment on Hydro-extraction of Water-Soluble Collagen from Skin of Alaska Pollock (Theragra chalcogramma) Patmawati; Aliffiansyah Rizky Ergion; Laksmi Sulmartiwi; Raseetha Vani Siva Manikam; Dwitha Nirmala; Yaowapha Waiprib; Sapta Wijayanti
Jurnal Ilmiah Perikanan dan Kelautan Vol. 15 No. 2 (2023): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v15i2.41274

Abstract

Highlight Research The optimal condition for production of water-soluble collagen from Alaska pollock fish skin was the pretreatment with 0.15 M acetic acid followed by hydro-extraction at 40oC with 150 rpm for two hours. Higher acetic acid concentration at a pretreatment step enhanced the higher yield of water-soluble collagen via hydro-extraction process. Hydro-extraction was an environmentally friendly method for converting Alaska pollock fish skin to water-soluble collagen. Alaska pollock fish skin could be used as an alternative source for collagen production. Abstract To date, there is no information on skin of Alaska Pollock (Theragra chalcogramma) being used as a source for collagen. In order to produce water-soluble collagen from the skin of Alaska Pollock, a process known as hydro-extraction is utilized. This technique does not need a long extraction time or a large amount of chemical reagent. The purpose of this study was to investigate the effect of acetic acid pretreatment on hydro-extraction of water-soluble collagen from Alaska Pollock skin. The skin samples were pretreated using acetic acid at different concentrations (0.01 M; 0.05 M; 0.1 M, 0.15 M) for two hours at chilling temperature. The pretreated skin samples were further processed to produce water-soluble collagen using the hydroxy-extraction method. The obtained collagen was analyzed for proximate compositions, yield, and amino acids compositions with high performance liquid chromatography. It was found that the proximate compositions of the collagen products, specifically the protein content (75%) and fat content (1%), met the requirements of the Indonesian National Standard (SNI 8076:2014). The collagen yield ranged from 2.6 to 3.13%. The predominate amino acids in collagen were glycine, arginine, proline, glutamic acid, serine, and alanine. Pretreatment of skin sample with 0.15 M acetic acid resulted in the highest yield of water-soluble collagen (3.13%) and protein content (91.13%). The skin of Alaska Pollock fish could be used as an alternative raw material to produce water-soluble collagen for medical, pharmacy or food processing applications.
Co-Authors Aliffiansyah Rizky Ergion Clara Amelia Kusumawinahyu Dwi Yuli Pudjiastuti Dwitha Nirmala Laksmi Sulmartiwi Mochammad Amin Alamsjah Raseetha Vani Siva Manikam Sapta Wijayanti Sharilla Aryananti Abidin Yaowapha Waiprib