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MULTIPLIKASI TUNAS DAN INDUKSI UMBI MIKRO SATOIMO (Colocasia esculenta (L.) Schott) PADA BEBERAPA KONSENTRASI SUKROSA DAN BENZILAMINOPURIN Maretta, Delvi; Handayani, Dwi Pangesti; Rosdayanti, Henti; Tanjung, Armelia
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol 3, No 2 (2016): December 2016
Publisher : Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (750.75 KB) | DOI: 10.29122/jbbi.v3i2.150

Abstract

Taro or Satoimo (Colocasia esculenta (L) Schott var antiquorum) is an alternative of non-rice food. To support saitomo mass cultivation in several regions in Indonesia, shoot multiplication and induction of satoimo microtuber through in vitro technique is amongst the stage to be undertaken. The aims of this study were to determine the effect of BAP (benzylaminopurine) and sucrose for shoot multiplication and microtuber induction of in vitro culture of satoimo. The experiment was arranged in two factors: BAP (0, 1, 2 and 3 mg/L) and sucrose (30, 60, 90 and 120 g/L). The result showed that the single effect of BAP or sucrose and interaction of both significantly increased the number of shoots. The effect of 2 mg/L BAP was more homogeneous than that of 1 and 3 mg/L BAP. Sucrose with the concentration of 30 g/L was the best concentration for shoot multiplication. The highest number of microtuber was achieved with 2 mg/L BAP + 30 g/L sucrose treatments, but tended to decrease due to increasing sucrose concentration. In 2 and 3 mg/L BAP treatments, the number of microtuber increased along with the increasing sucrose concentration.Keywords: satoimo, in vitro shoot, microtuber, benzylaminopurine, sucrose ABSTRAKSatoimo (Colocasia esculenta (L) Schott var antiquorum) merupakan bahan pangan alternatif non-beras. Untuk mendukung produksi massal satoimo di beberapa wilayah di Indonesia, multiplikasi tunas dan induksi umbi mikro secara in vitro merupakan tahapan yang harus dilakukan. Tujuan penelitian ini adalah untuk mengetahui pengaruh BAP dan sukrosa terhadap multiplikasi tunas dan induksi umbi mikro satoimo dalam kultur in vitro. Perlakuan terdiri dari 4 taraf konsentrasi BAP (0, 1, 2 dan 3 mg/L) dan 4 taraf konsentrasi sukrosa (30, 60, 90 dan 120 g/L). Hasil penelitian menunjukkan bahwa BAP dan sukrosa secara tunggal serta interaksinya berpengaruh nyata terhadap multiplikasi tunas in vitro. Pengaruh konsentrasi BAP 2 mg/L lebih homogen dibandingkan perlakuan BAP 1 dan 3 mg/L. Sukrosa 30 g/L merupakan konsentrasi terbaik untuk multiplikasi tunas. Umbi mikro terbanyak terdapat pada perlakuan BAP 1 mg/L + sukrosa 30 g/L tetapi cenderung mengalami penurunan jika konsentrasi sukrosa dinaikkan pada konsentrasi BAP tetap. Pada perlakuan BAP 2 dan 3 mg/L jumlah umbi mikro yang terbentuk cenderung meningkat seiring dengan peningkatan konsentrasi sukrosa.Kata kunci: satoimo, tunas in vitro, umbi mikro, benzilaminopurin, sukrosa
MULTIPLIKASI TUNAS DAN INDUKSI UMBI MIKRO SATOIMO (Colocasia esculenta (L.) Schott) PADA BEBERAPA KONSENTRASI SUKROSA DAN BENZILAMINOPURIN Maretta, Delvi; Handayani, Dwi Pangesti; Rosdayanti, Henti; Tanjung, Armelia
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 3 No. 2 (2016): December 2016
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (750.75 KB) | DOI: 10.29122/jbbi.v3i2.150

Abstract

Taro or Satoimo (Colocasia esculenta (L) Schott var antiquorum) is an alternative of non-rice food. To support saitomo mass cultivation in several regions in Indonesia, shoot multiplication and induction of satoimo microtuber through in vitro technique is amongst the stage to be undertaken. The aims of this study were to determine the effect of BAP (benzylaminopurine) and sucrose for shoot multiplication and microtuber induction of in vitro culture of satoimo. The experiment was arranged in two factors: BAP (0, 1, 2 and 3 mg/L) and sucrose (30, 60, 90 and 120 g/L). The result showed that the single effect of BAP or sucrose and interaction of both significantly increased the number of shoots. The effect of 2 mg/L BAP was more homogeneous than that of 1 and 3 mg/L BAP. Sucrose with the concentration of 30 g/L was the best concentration for shoot multiplication. The highest number of microtuber was achieved with 2 mg/L BAP + 30 g/L sucrose treatments, but tended to decrease due to increasing sucrose concentration. In 2 and 3 mg/L BAP treatments, the number of microtuber increased along with the increasing sucrose concentration.Keywords: satoimo, in vitro shoot, microtuber, benzylaminopurine, sucrose ABSTRAKSatoimo (Colocasia esculenta (L) Schott var antiquorum) merupakan bahan pangan alternatif non-beras. Untuk mendukung produksi massal satoimo di beberapa wilayah di Indonesia, multiplikasi tunas dan induksi umbi mikro secara in vitro merupakan tahapan yang harus dilakukan. Tujuan penelitian ini adalah untuk mengetahui pengaruh BAP dan sukrosa terhadap multiplikasi tunas dan induksi umbi mikro satoimo dalam kultur in vitro. Perlakuan terdiri dari 4 taraf konsentrasi BAP (0, 1, 2 dan 3 mg/L) dan 4 taraf konsentrasi sukrosa (30, 60, 90 dan 120 g/L). Hasil penelitian menunjukkan bahwa BAP dan sukrosa secara tunggal serta interaksinya berpengaruh nyata terhadap multiplikasi tunas in vitro. Pengaruh konsentrasi BAP 2 mg/L lebih homogen dibandingkan perlakuan BAP 1 dan 3 mg/L. Sukrosa 30 g/L merupakan konsentrasi terbaik untuk multiplikasi tunas. Umbi mikro terbanyak terdapat pada perlakuan BAP 1 mg/L + sukrosa 30 g/L tetapi cenderung mengalami penurunan jika konsentrasi sukrosa dinaikkan pada konsentrasi BAP tetap. Pada perlakuan BAP 2 dan 3 mg/L jumlah umbi mikro yang terbentuk cenderung meningkat seiring dengan peningkatan konsentrasi sukrosa.Kata kunci: satoimo, tunas in vitro, umbi mikro, benzilaminopurin, sukrosa
RESPONS PERTUMBUHAN BIBIT KENTANG (Solanum tuberosum) TERHADAP FORMULASI BIOSTIMULAN BERBASIS Trichoderma spp. Nawfetrias, Winda; Handayani, Dwi Pangesti; Bidara, Irna Surya; Tanjung, Armelia
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 2 (2019): December 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (326.958 KB) | DOI: 10.29122/jbbi.v6i2.3710

Abstract

Growth Responses of Potato (Solanum tuberosum) Plantlets upon Application of Different Formulations of Trichoderma spp.-Based BiostimulantPlantlet acclimatization is one of several critical steps in potato clonal seedling production which often hampers the availability of quality plant materials. Application of biostimulant formulation containing Trichoderma spp. may increase growth capability of potato plantlets due to the improvement of plantlets in absorbing nutrient from the growth media. The aim of this research was to obtain the best formulations of biostimulant containing several strains of Trichoderma spp. to be applied in the acclimatization stage of potato seeds. Nine formulations of biostimulant (i.e. S1, S2, S3, S4, S5, S6, S7, S8, S9) each of which contained three to four different strains of five Trichoderma spp. isolates, were mixed with organic substrate carriers. The biostimulants were then applied to potato plantlets cv. Atlantic at the beginning of acclimatization. Plantlets without biostimulant were used as the control treatment (S0). The results showed that the S8 biostimulant formulation significantly increased the growth of the potato plantlets. The results of this experiment indicated that compared with the control and other formulations, the S8 biostimulant formulation significantly increased the growth of potato plantlets based on the number of leaves and number of shoots.Keywords: aclimatitation; biostimulant; potato; seedling; TrichodermaABSTRAKAklimatisasi planlet adalah salah satu tahapan kritis dalam produksi bibit klonal kentang yang seringkali menghambat ketercukupan bahan tanaman berkualitas. Penggunaan produk berbasis Trichoderma spp. diduga dapat meningkatkan kemampuan planlet untuk menyerap unsur hara dari media tumbuh. Tujuan penelitian ini adalah untuk mengetahui formulasi biostimulan terbaik yang mengandung Trichoderma spp. yang diaplikasikan pada tahap aklimatisasi bibit kentang. Sembilan biostimulan (S1, S2, S3, S4, S5, S6, S7, S8, S9) diformulasikan menggunakan substrat pembawa organik dan masing-masing mengandung campuran tiga atau empat strain dari lima isolat Trichoderma spp. Kesembilan biostimulan tersebut kemudian diujikan pada planlet kentang varietas Atlantik siap aklimatisasi. Planlet tanpa aplikasi biostimulan digunakan sebagai kontrol (S0). Hasil percobaan ini menunjukkan bahwa dibandingkan dengan control maupun formulasi lainnya, formulasi biostimulan S8 secara signifikan meningkatkan pertumbuhan planlet kentang cv. Atlantik dilihat dari jumlah daun dan jumlah tunasnya.