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Optimization and Validation of Electrochemical Biosensor Based on Pseudomonas sp. Biofilm Immobilized on Screen-Printed Carbon Electrode in Detecting Benzene Alfiah Alif; Dyah Iswantini; Henny Purwaningsih; Novik Nurhidayat; Ali Aulia Ghozali; Amalyah Febryanti
Jurnal Akta Kimia Indonesia (Indonesia Chimica Acta) Volume 15, No 1: June 2022
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ica.v15i1.19008

Abstract

Benzene is known as one of the hazardous compounds potentially interfering the health and polluting the environment. Generally, detecting benzene still requires a long analysis time and expensive costs. Therefore, this study aimed to evaluate the analytical performance of a biosensor with microbes as the biodetection element. Pseudomonas sp. biofilms which produce benzene dioxygenase enzyme and then immobilized on a Screen-Printed Carbon Electrode (SPCE). The results of the optimization of the biosensor obtained a benzene concentration of 3 mM, a bacterial density of 1.4x1011 cells/mL and suspension pH of 7.5. The optimization results are used to measure the analytical performance of the biosensor. The value of analytical performance produced on linearity is in the measurement range of 0.1 - 3 mM, the equation y = 7.4118x + 80.048 with R2 = 0.9744. The detection limit and quantity limit are 0.5630 mM and 1.8767 mM respectively, with a sensitivity of 7.4188 μA/mM. The precision obtained shows that the SPCE biofilm method has moderate accuracy with a %SBR value below 5%. The selectivity of this method still needs to increase, but the stability of the benzene biosensor increases up to 35 days with an activity of 100.36%. This indicates the immobilization of Pseudomonas sp. had potency as an alternative method for detecting benzene and it can be developed for a prototype.
Performance of Sediment Microbial Fuel Cells in Generating Electricity using Fish Wastewater and Shrimp Wastewater as a Nutrient and Their Effect on Waste Quality Alfiah Alif; Muhamad Jalil Baari; Amalyah Febryanti
International Journal of Science, Technology & Management Vol. 4 No. 1 (2023): January 2023
Publisher : Publisher Cv. Inara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46729/ijstm.v4i1.673

Abstract

Sediment Microbial Fuel Cell (MFC) is a technology that can convert chemical energy into electrical energy through the process of nutrient degradation by microbes. Sediment taken from the bottom of shrimp ponds was added as a source of microbes, while fish and shrimp wastewater were used as a source of nutrients for microbes. This study aims to measure the performance of the SMFC system on fish effluent and shrimp effluent to produce bio-electricity while reducing the waste load. The research method was experimental laboratories. The treatment given was the different types of electrodes, namely zinc-copper and aluminum-copper. In addition, 0.2 M KMnO4 electrolyte solution was used. This study consisted of four stages: the manufacture of nutrients from fish and shrimp wastewater, the manufacture of a dual chamber MFC bioreactor, the measurement of electrical values, and the analysis of waste quality. Experiments were carried out for 30 days by measuring electricity every 24 hours. The average value of electricity generated in the nutrients of fish wastewater with Zn/Cu electrodes was 0.705 V and Al/Cu was 0.472 V. Meanwhile, the average value of electricity in shrimp wastewater nutrients with Zn/Cu electrodes was 0.630 V and Al/Cu was 0.625 V. The number of colonies after adding sediment in the shrimp wastewater sample were 8.9 x 106 CFU/mL, the fish wastewater sample was 9.5 x 106 CFU/mL. It indicates the presence of microorganisms that play a role in the SMFC system