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All Journal Jurnal Penelitian Pendidikan IPA (JPPIPA) JPBIO (Jurnal Pendidikan Biologi) Medical Scope Journal (MSJ)
Halidah Rahawarin
Universitas Pattimura
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Health Professions Immunology & microbiology Materials Science & Nanotechnology Nursing Physics Public Health

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Pap Smear as A Cervical Cancer Screening in Women of Childbearing Age in 2020. Merlin Margreth Maelissa; Halidah Rahawarin; Nathalie Elischeva Kailola; Vina Zakiah Latuconsina; Ninik Ma’athia Sallatalohy
Jurnal Penelitian Pendidikan IPA Vol. 8 No. 4 (2022): October
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v8i4.2074

Abstract

Early detection through screening programs is one of the cervical cancer prevention strategies that can help reduce cervical cancer morbidity and mortality. One method of early detection is through Pap smear examination. This study aimed to determine the profile of pap smear results at the Nusaniwe Sub-District Health Center, Ambon City. This descriptive observational study with a cross-sectional design was conducted at Amahusu Health Center, Benteng Health Center, Waihaong Health Center, and Urimessing Health Center. Pap smear examination was performed on women of childbearing age who came for treatment at the Health Center. They had no contraindications for pap smear examination and the results of the nonreactive IgM and IgG Sars-CoV2 antibody tests. From the examination results, on a total of 38 samples, the average age of the sample was 36.89, with the youngest age being 24 years old and the oldest being 46 years old. The highest age group was >35 years (55.3%), and the most age at first sexual intercourse was in the > 20-year age group (68.4%). Then, the most parity status was the multiparous group (68.4%). Generally, the sample had never undergone cervical cancer screening, both VIA examination and previous pap smear examination (60% and 92.1%, respectively). The most pap smear results were inflammation with infectious organisms as much as 42.1%, followed by inflammation (39.5%), Negative for Intraepithelial Lesion or Malignancy (10.5%), Atypical Squamous Cells of Undetermined Significance (5.3%) and Low-Grade Squamous Intraepithelial Lesion (2.6%). The most infecting organisms were bacteria (21.1%).
The potential of endophytic bacteria isolated from atung fruit seeds as antibacterial against skin pathogens Melda Yunita; Adelia Mantong; Halidah Rahawarin
JPBIO (Jurnal Pendidikan Biologi) Vol 8, No 1 (2023): April 2023
Publisher : STKIP Persada Khatulistiwa Sintang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31932/jpbio.v8i1.2271

Abstract

Antibiotic resistance is one of the biggest threats to global health today. Estimated by 2050, antibiotic resistance will cause around 10 million deaths per year if alternative treatments have not been identified. This study was aimed to determine the potential of endophytic bacterial isolates from the seeds of atung fruit (Parinarium glaberrimum Hassk) as an antibacterial against skin pathogens Cutibacterium acnes and MDR Staphylococcus aureus, and Staphylococcus epidermidis. This research was a descriptive observational research with laboratory experimental methods. This study used isolates of endophytic bacteria isolated from the seeds of atung fruit which were tested with pathogenic bacteria. The results showed that 2 of 7 endophytic bacterial isolates were able to inhibit the growth of C. acnes with clear zone diameter of 4 mm and 9 mm, respectively, yet couldn’t able to inhibit MDR S. aureus and S. epidermidis. This study concluded that the 2 isolates (isolate BBA5 and BBA9) could have potential as antibacterial against C. acnes. While 5 other isolates had not been able to inhibit the whole pathogenic bacteria tested. An optimization is necessary in an effort to develop these isolates as antibacterial.
Perbandingan Efektivitas Daya Antibakteri Minyak Seith dengan Minyak Bawang Putih (Allium sativum) terhadap Pertumbuhan Pseudomonas aeruginosa Siti N. Aihena; Yuniasih M. J. Taihuttu; Halidah Rahawarin
Medical Scope Journal Vol. 6 No. 1 (2024): Medical Scope Journal
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35790/msj.v6i1.48723

Abstract

Abstract: Pseudomonas aeruginosa is an opportunistic bacterium and has the ability to withstand several types of antibiotics (antibiotic resistance). Garlic oil contains active substances such as flavonoids, alkaloids, saponins, tannins, and curcumin which function as antibacterial. This study aimed to compare the antibacterial effectiveness of Seith oil with of garlic oil (Allium sativum) against P. aeruginosa bacteria. The oil concentrations were 10%, 15%, 20%, 25%, 50%, 75%, and 100%. Antibacterial activity test against P. aeruginosa bacteria was assessed using the disk diffusion method. The results showed that Seith oil could not inhibit the growth of P. aeruginosa, meanwhile garlic oil of 50%, 75%, and 100% inhibited the growth of P. aeruginosa with the mean inhibition zone diameters of 1.38 mm, 1.88 mm, and 3.13 mm, respectively. The Kruskal-Wallis test showed a p-value of <0.01 indicating significant difference in the diameters of inhibition zones between Seith oil and garlic oil (Allium sativum) against P. aeruginosa bacteria. The Mann-Whitney U test showed significant differences between garlic oil (Allium sativum) of 100% and 75% against Pseudomonas aeruginosa bacteria, and between garlic oil of 75% and 50%. In conclusion, garlic oil (Allium sativum) is more effective than Seith oil against Pseudomonas aeruginosa bacteria. Keywords: Seith oil; garlic oil (Allium sativum); Pseudomonas aeruginosa; antibacterial effect   Abstrak: Pseudomonas aeruginosa merupakan bakteri oportunistik dengan kemampuan bertahan terhadap beberapa jenis antibiotik (resisten antibiotik). Minyak bawang putih memiliki kandungan zat aktif seperti flavonoid, alkaloid, saponin, tanin, dan kurkumin yang berfungsi sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui perbandingan efektivitas daya antibakteri antara minyak seith dengan minyak bawang putih (Allium sativum) terhadap bakteri Pseudomonas aeruginosa. Konsentrasi minyak yang digunakan ialah 10%, 15%, 20%, 25%, 50%, 75%, dan 100%. Uji aktivitas antibakteri terhadap bakteri P. aeruginosa menggunakan metode difusi cakram. Hasil penelitian memperlihatkan bahwa minyak Seith tidak dapat menghambat pertumbuhan bakteri P. aeruginosa. Minyak bawang putih menghambat pertumbuhan bakteri P. aeruginosa pada konsentrasi 50%, 75%, dan 100% dengan masing-masing rerata diameter zona hambat ialah 1,38 mm, 1,88 mm, dan 3,13 mm. Hasil uji Kruskal-Wallis menunjukkan nilai p<0,001 yang berarti terdapat perbedaan diameter zona hambat yang bermakna antara minyak Seith dan minyak bawang putih (Allium sativum). Efektivitas daya antibakteri minyak bawang putih (Allium sativum) lebih besar dibanding minyak Seith. Hasil uji Mann-Whitney U menunjukkan bahwa minyak bawang putih dengan konsentrasi 100% dan 75% memiliki perbedaan bermakna (p=0,015), serta konsentrasi 75% dan 50% (p=0,040). Simpulan penelitian ini ialah daya antibakteri minyak bawang putih (Allium sativum) lebih efektif dibandingkan daya antibakteri minyak Seith terhadap bakteri Pseudomonas aeruginosa. Kata kunci: minyak Seith; minyak bawang putih (Allium sativum); Pseudomonas aeruginosa; efek antibakteri
Co-Authors Adelia Mantong Merlin Margreth Maelissa Nathalie Elischeva Kailola Ninik Ma’athia Sallatalohy Siti N. Aihena Vina Zakiah Latuconsina Yuniasih M. J. Taihuttu Yunita, Melda