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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences
Olanike Maria Buraimoh
UNIVERSITY OF LAGOS
Agriculture, Biological Sciences & Forestry Environmental Science

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Pectinase Production by Bacillus and Paenibacillus sp. Isolated from Decomposing Wood Residues in the Lagos Lagoon Busayo Tosin Akinyemi; Olanike Maria Buraimoh; Olayemi Omowunmi Ogunrinde; Olukayode Oladipo Amund
Journal of Tropical Life Science Vol. 7 No. 3 (2017)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.07.03.03

Abstract

Three wood decomposing bacteria isolated from the Lagos lagoon, Bacillus megaterium, Bacillus bataviensis and Paenibacillus sp. were screened for their pectinase producing abilities using pectin as substrate under submerged fermentation (SMF) conditions. The results showed that all three isolates produced appreciable pectinolytic activities. Paenibacillus sp. showed the highest pectinase activity when compared with the other two isolates. The optimum pH for pectinase activity for both B. megaterium and B. bataviensis was 8.0 while it was 6.5 for Paenibacillus sp., B. bataviensis, and B. megaterium showed optimum pectinase activity at 60°C and Paenibacillus sp. at 40°C. Metal ions such as Na+ and K+ improved the activity of pectinase produced by the three isolates when compared to the effect of Zn2+ and Mn2+. The molecular weights of the enzymes were also estimated by gel filtration as 29,512 da, 32,359 da, and 25,119 da for Paenibacillus sp., B. megaterium and B. bataviensis respectively. The study has provided a platform for further investigation into the biochemical characterization of the enzyme, and optimization of culture conditions to scale up pectinase production for commercial exploitation.
Potentials of Hydrocarbon Utilization by Wood-Digesting Bacteria isolated from the Lagos Lagoon, Nigeria. Olanike Maria Buraimoh; Adewale Kayode Ogunyemi; Isaac Edward Oyegbite; Olukayode Oladipo Amund; Matthew Olusoji Ilori
Journal of Tropical Life Science Vol. 10 No. 1 (2020)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.10.01.01

Abstract

Introduction: There is a continuous search for environmental–friendly methods to address oil-polluted environments, hence this study aimed to establish the potential of using tropical estuarine bacterial strains for degradation of crude oil in polluted environment. Microbial degradation of hydrocarbons has been considered a promising, natural, less toxic and cost-effective technology. Lignocellulose-utilizing bacterial strains (Bacillus megaterium strain NOB, Streptomyces pseudogriseolus strain EOB and Paenibacillus sp. strains ROB) were isolated from decomposing wood residues in a tropical lagoon. They were identified on the basis of morphological and biochemical characteristics, observation under scanning electron microscope and 16S rRNA gene sequencing. Methods; Microbial growth assessment coupled with biosurfactant production were performed in triplicates under aerobic batch conditions in Erlenmeyer flasks containing mineral salts medium fortified with trace elements and crude oil as the sole carbon source.  Results: The generation times of strains NOB, EOB and ROB were 28.3, 44.9 and 46.8 h; with specific growth rates of 14.2, 8.9 and 8.6 h-1,respectively on crude oil. Gas chromatographic analysis of residual hydrocarbons from the growth cultures of isolates revealed that the three strains had degraded the oil by 89.34, 86.33 and 79.37%, respectively at the end of 21 day. The cell hydrophobicity of the bacterial strains also reached 83.6, 79.4 and 69.4%, respectively. Conclusion: The results from this study suggest that wood-digesting bacterial strains from a tropical lagoon possess hydrocarbon utilization capabilities. They could therefore have potentials for deployment in the bioremediation of petrochemical spills in polluted environments.
Co-Authors Adewale Kayode Ogunyemi Busayo Tosin Akinyemi Isaac Edward Oyegbite Matthew Olusoji Ilori Olayemi Omowunmi Ogunrinde Olukayode Oladipo Amund