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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences The Journal of Experimental Life Sciences (JELS) E-Dimas: Jurnal Pengabdian kepada Masyarakat Indonesian Journal of Electrical Engineering and Computer Science
Nashi Widodo
Universitas Brawijaya
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Medicine & Pharmacology Neuroscience Social Sciences

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in silico Study Reveals Potential Docking Sites of δ 2-isoxazolines derivates for Inhibiting Russell’s Viper PLA2 Toxin Tsaniyah Nur Kholilah; Nashi Widodo; Nia Kurniawan
Journal of Tropical Life Science Vol. 11 No. 1 (2021)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.11.01.06

Abstract

Snake venom phospholipase A2s (svPLA2s) has been known as the most abundant component and predominant cause of Russell’s viper envenomation. Limitation to serum therapy and considerable pharmacological interest led the researcher to synthesized multi-toxic PLA2 inhibitors, δ2-isoxazolines derivate. Although δ2- isoxazolines derivate already proved inhibitor activity in Group II svPLA2 with known IC50, their mechanism of action remains unveiled. Our recent study investigated their inhibitory activity via molecular docking. The virtual screening revealed that the ligand with diverse structures tied to the relatively same active site region. The result sheds light on the significance of His48 and Asp49 as part of the pro-inflammatory eliciting region. ADME analysis was also performed to filter and identify the best potential inhibitor acceptable for human use. This moiety leads to finding a better therapeutic strategy of svPLA2 inhibitors both as an alternative to serum anti-venom treatment.
Piper betle L. Leaves Extract Potentially Reduce the Nitric Oxide Production on LPS-Induced RAW 264.7 Cell Lines Dinia Rizqi Dwijayanti; Sapti Puspitarini; Nashi Widodo
The Journal of Experimental Life Science Vol. 13 No. 2 (2023)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2023.013.02.02

Abstract

Chronic inflammation can lead to several diseases that represent the leading causes of mortality worldwide. Conventional treatment of inflammation can carry some risks. Therefore, research on herbal medicine that are suspected of having anti-inflammatory effects, such as Piper betle L., is important. This study aims to investigate the effect of P. betle L. extract on nitric oxide as a pro-inflammatory mediator. The dried leaves of P. betle L. were extracted by ethanol. RAW 264.7 cells were treated with LPS and P. betle L. extract (PBE). Nitric oxide was measured by the Griess method. Antioxidant activity was determined using 2,2–Diphenyl-1-picrylhydrazyl (DPPH) method. Total flavonoids and phenolic content were also identified by aluminium chloride and Folin–Ciocalteu colorimetric assay, respectively. This study demonstrated that the PBE has excellent NO suppression activity with the IC50 56.22±16.41 μg.mL-1, without cytotoxicity. PBE also has DPPH inhibitory concentration IC50 values of 279.67±11.36 ppm. Interestingly, PBE has a flavonoid content of 50.17±3.14 mg QE.g-1 and phenolic content of 128.92±1.2 mg GAE.g-1. These compounds are thought to be responsible for its anti-inflammatory as well as antioxidant. This study proved that P. betle L. leaves extract could be used as a candidate for anti-inflammatory drugs. Nevertheless, further research about the biological activity mechanism and their bioactive compounds' purification is still required. Keywords: Inflammation, lipopolysaccharide, Macrophage, Nitric Oxide, Piper betle. 
Pendalaman Imunologi/Vaksin dan Pewarnaan Intraseluler bagi Guru SMA/Sederajat se-Malang Raya dan Sekitarnya Muhaimin Rifa'i; Muhammad Sasmito Djati; Nashi Widodo; Sefihara Paramitha Sakti; Fikriya Novita Sari; Farida Rachmawati; Aya Shofiyah; Kavana Hafil Kusuma
E-Dimas: Jurnal Pengabdian kepada Masyarakat Vol 14, No 4 (2023): E-DIMAS
Publisher : Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/e-dimas.v14i4.13509

Abstract

Malang merupakan salah satu kota pelajar yang memiliki berbagai sekolah dan perguruan tinggi yang terpercaya bagi pelajar dari berbagai daerah untuk mendapatkan pendidikan yang berkualitas. Pembelajaran di tingkat SMA bukan hanya terfokus pada kegiatan belajar mengajar, namun juga pada keterampilan laboratorium yang dapat meningkatkan kemampuan siswa terutama pada bidang biologi. Peningkatan minat siswa pada bidang sains mendorong para guru untuk lebih kreatif mengembangkan ilmu pengetahuan siswa mengikuti perkembangan teknologi. Pengabdian masyarakat ini diselenggarakan sebagai media transfer ilmu Biologi terbaru, terutama terkait imunologi dan pewarnaan intraseluler, kepada guru SMA/sederajat se-Malang Raya dan sekitarnya. Tahapan kegiatan diawali dengan persiapan dan sosialisasi program kepada peserta, perumusan materi, pelaksanaan kegiatan berupa pemaparan materi dan praktikum, serta evaluasi. Pendalaman konsep vaksin dan imunologi serta konsep pewarnaan intraseluler menggunakan flow cytometry telah terlaksana dengan hasil evaluasi menunjukkan adanya peningkatan pemahaman peserta dalam penguasaan materi yang diberikan. Evaluasi pada kegiatan menunjukan 93% peserta sangat berminat dan menunjukan tingkat kepuasan peserta pada kegiatan yang dilakukan. Kegiatan ini meningkatkan pemahaman peserta pada konsep imunologi dan vaksin sebagai bekal pembelajaran siswa. Kegiatan serupa sangat diharapkan oleh peserta untuk dilakukan secara berkelanjutan dengan pemahaman yang lebih kompleks.
Single nucleotide polymorphism based on hypertension potential risk prediction using LSTM with Adam optimizer Lailil Muflikhah; Imam Cholissodin; Nashi Widodo; Feri Eko Herman; Teresa Liliana Wargasetia; Hana Ratnawati; Riyanarto Sarno
Indonesian Journal of Electrical Engineering and Computer Science Vol 33, No 2: February 2024
Publisher : Institute of Advanced Engineering and Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11591/ijeecs.v33.i2.pp1126-1139

Abstract

Recent healthcare research has focused a great deal of interest on using genetic data analysis to predict the risk of hypertension. This paper presents a unique method for accurately predicting the vulnerability to hypertension by utilizing single nucleotide polymorphism (SNP) data. We present a novel neural network design utilizing the adaptive moment (Adam) optimizer to describe the intricate temporal correlations in SNPs. The study used a dataset with carefully preprocessed SNP data from a broad cohort for model input. The long short-term memory (LSTM) network was methodically built and trained with hyper-parameter and fine-tuning using the Adam optimizer to converge on ideal weights. Our findings indicate encouraging predictive performance, highlighting the suggested methodology’s usefulness in determining hypertension risk factors. The result showed that the proposed method achieved stability in the performance of 89% accuracy, 96% precision, 88% recall, and 92% F1-score. Due to its higher accuracy and greater predictive power, our SNP-based LSTM methodology is superior to the conventional machine learning method. By providing a novel framework that uses genetic data to predict the risk of hypertension, this research makes substantial contribution to the field of predictive healthcare. This framework helps with early intervention and customized preventative efforts.
Co-Authors Aya Shofiyah Dinia Rizqi Dwijayanti Farida Rachmawati Feri Eko Herman Fikriya Novita Sari HANA RATNAWATI Imam Cholissodin Kavana Hafil Kusuma Lailil Muflikhah Muhaimin Rifa'i Muhammad Sasmito Djati Nia Kurniawan Riyanarto Sarno Sapti Puspitarini Sefihara Paramitha Sakti Tsaniyah Nur Kholilah