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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences
Luqman Arif Zulkarnain
Universiti Teknologi Malaysia
Agriculture, Biological Sciences & Forestry Environmental Science

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Genomic Analysis for Haloacid Dehalogenase in Bacillus megaterium WSH-002 Luqman Arif Zulkarnain; Fahrul Zaman Huyop
Journal of Tropical Life Science Vol. 12 No. 1 (2022)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.12.01.07

Abstract

Bacterial dehalogenation is one of the processes that can reduce the environmental pollutions. The attributes of Bacillus megaterium sp. that able to grow in polluted environment suggested that, its genome contains pollutant degrading genes. To date, there were no reports related to dehalogenase in Bacillus megaterium WSH-002 and how it was regulated. Therefore, the presence of environmentally important genes that can detoxify organohalogens in many microbial genomes including Bacillus megaterium WSH-002 will be investigated. The results from genome annotations of Bacillus megaterium WSH-002 has shown its potential for bioremediation due to the putative haloacid dehalogenase proteins. Only one type of haloacid dehalogenase was identified. It was classified as haloacid dehalogenase type II because of its amino acid sequence are highly identical with HAD_type_II and HAD_L2-DEX. The study concluded that, the genome of Bacillus megaterium WSH-002 contains haloacid dehalogenase genes that is useful for biodegradation of halogenated compound. In future, further investigation on the expression of the dehalogenase gene as recombinant protein and bioinformatic analysis to study dehalogenase protein structure and functions will be considered. 
The Genetic Organisation and Control of Putative Dehalogenase Gene Expression in Bacillus megaterium WSH-002: Putative Dehalogenase Gene Expression in Bacillus megaterium Luqman Arif Zulkarnain; Fahrul Huyop
Journal of Tropical Life Science Vol. 13 No. 2 (2023)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.13.02.15

Abstract

Dehalogenase-producing bacterium has been extensively studied due to their ability to reduce pollution in the environment. A previous study reported that Bacillus megaterium WSH-002 contains a putative haloacid dehalogenase type II gene, therefore, the presence of other genes associated with the dehalogenase regulatory gene function and uptake of halogenated compounds are expected. In the Bacillus megaterium WSH-002 whole genome, type II dehalogenase (DehWSH002) and two other genes related to dehalogenase regulatory and haloacid uptake genes were identified suggesting its ability to control the expression of putative dehalogenase(s) and the uptake of halogenated compounds into the cells. The phylogenetic analysis showed that DehWSH002 shared common features with DehLBHS1 of Bacillus megaterium strain BHS1 and Had protein of Bacillus megaterium strain ATCC12872/QMB1551. The study concluded that the genome of Bacillus megaterium WSH-002 contains a dehalogenase gene designated as dehWSH002 that is useful for biodegradation. In addition, further investigation of the adjacent genes suggested the presence of dehalogenase regulatory gene (dehR) and an uptake gene (dehP) in a single genetic organisation.
Co-Authors Fahrul Huyop Fahrul Zaman Huyop