Article Per Year (5 Year)
p-Index From 2019 - 2024
0.444
P-Index
This Author published in this journals
All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences
Nurul Hidayah Samsulrizal
Nurul Hidayah Binti Samsulrizal Assistant Professor Kullyyiah of Science IIUM Kuantan Campus hidayahsamsulrizal@iium.edu.my 5158
Agriculture, Biological Sciences & Forestry Environmental Science

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
In Silico Analysis and 3D Structure Prediction of Putative UDP-Glycosyltransferase 76G1 Protein in Stevia rebaudiana MS007: In Silico Analysis and 3D Structure Prediction of Putative UDP-Glycosyltransferase 76G1 Nor Iswani Mokthar; Muhammad Amirul Husni Samsulrizal; Afiqah Ramatullah Khan; Zarina Zainuddin; Tamil Chelvan Meenakshi Sundram; Nik Yusnoraini Yusof; Nurul Hidayah Samsulrizal
Journal of Tropical Life Science Vol. 12 No. 3 (2022)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.12.03.11

Abstract

Stevia rebaudiana is a plant of the Asteraceae family that is used as a natural sweetener. Stevia has been shown to be safe for human consumption and has been utilised as a sweetener substitute for diabetic and obese people. In this study, the structure and gene content involved in the synthesis of putative UDP-glycosyltransferase 76G1 (UGT76G1) protein in S. rebaudiana MS007 was analyzed using an in silico method. Homologous search using blastP revealed the highest percentage of identity, score, and E-value for UDP-glycosyltransferase 76G1-like of Helianthus annuus (ID: XP_021973845.1). The presence of IPR002213 UDP-glucuronosyl/UDP-glucosyltransferase entry, which is available at locations 89bp to 246bp, was also verified by the protein family search using InterPro. MEGA-X software was used to construct a molecular phylogeny study, which revealed that this protein belongs to the Asteraceae protein family. To predict the primary, secondary, and tertiary protein structures of the putative UGT76G1 protein, the ProtParam, ExPasy, PSIPRED, and Phyre2 programs were implemented. The putative UGT76G1 protein’s tertiary structure prediction was given a score of 100.0% confidence by the single highest scoring template and a coverage of 98% with the dimension of the model being (Å) of X: 52.453, Y: 61.270, and Z: 48.102. The UGT76G1model fulfilled the quality standards and was approved for further analysis after validation performed by PROCHECK, VERIFY3D, and ERRAT. Thus, the findings of this work have contributed to a better knowledge of putative UDP-glycosyltransferase 76G1 features and target recognition processes, which will lead to a better information of protein-protein interaction in S. rebaudiana MS007.
Development of CRISPR/Cas9 Construct in Rice (Oryza sativa subsp. indica) Using Golden Gate Cloning Method Towards Drought Tolerance: Development of CRISPR/Cas9 Construct in Rice (Oryza sativa subsp. indica) Using Golden Gate Cloning Method Towards Drought Tolerance Nurul Hidayah Samsulrizal; Anis Afuza Md Yusof; Amin-Asyraf Tamizi; Nurul Asyikin Mohd Zim; Siti Syafiqa Abdul Sattar; Mohd Syahmi Salleh; Nur Sabrina Ahmad Azmi; Zamri Zainal; Zarina Zainuddin
Journal of Tropical Life Science Vol. 13 No. 2 (2023)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.13.02.04

Abstract

Rice (Oryza sativa) is a staple food consumed by the majority of the world’s population. Climate change, however, has created a significant threat to our food security as it posed severe effects on rice production. The emergence of genome editing technology has opened a new era in crop improvement. Hence, this study aims to develop the CRISPR/Cas9 construct of drought tolerance for O. sativa subsp. indica cv. IR64 using Golden Gate cloning method. For this purpose, the generation of CRISPR/Cas9 constructs involved several stages, i.e., characterization of SUMO E2-Conjugating Enzyme (OsSCE1) gene, single-guide RNA (sgRNA) design and vector construction. FGENESH, GeneMarkS, InterProScan, and Blast2GO programmes – were used for the OsSCE1 gene characterisation. The putative OsSCE1 gene isolated from IR64 was then verified by sequencing, and the gene was 585 bp long and showed 99% identity with O. sativa on chromosome 10. In silico analysis concluded the gene is involved in abiotic stress regulation. The 20 bp sgRNA was designed manually with the aid of gRNA prediction programmes including CCTop, and Benchling. The virtual vector was validated using the Golden Gate Cloning approach and later confirmed through sequencing. The assembly involved separate vectors containing the OsSCE1 sgRNA sequence, plant selectable marker, and Cas9 cassette to construct standardised elements for hierarchical modular cloning (MoClo). This study demonstrated that our format, as the gene insertion are achievable, resulting in a speedier and more efficient assembly process which may contribute to improve drought tolerance in indica rice. Further study on the Agrobacterium-mediated transformation using the developed construct may be conducted to determine the efficacy of knocking out candidate genes in improving drought tolerance ability O. sativa
Co-Authors Afiqah Ramatullah Khan Amin-Asyraf Tamizi Anis Afuza Md Yusof Mohd Syahmi Salleh Muhammad Amirul Husni Samsulrizal Nik Yusnoraini Yusof Nor Iswani Mokthar Nur Sabrina Ahmad Azmi Nurul Asyikin Mohd Zim Siti Syafiqa Abdul Sattar Tamil Chelvan Meenakshi Sundram Zamri Zainal Zarina Zainuddin