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Deiske Adeliene Sumilat
Sam Ratulangi University
Environmental Science

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Identification of Bioactive Compounds and Antibacterial Activity of Sea Cucumber, Holothuria (Halodeima) atra Jaeger 1833 Flesh Extract from Kalasey Coastal Waters, Minahasa District Theresia Tessa Mewengkang; Rosita Anggreiny Lintang; Fitje Losung; Deiske Adeliene Sumilat; Lawrence J. L Lumingas
Jurnal Ilmiah PLATAX Vol. 10 No. 2 (2022): ISSUE JULY-DECEMBER 2022
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.v10i2.42271

Abstract

Sea cucumbers are one of the marine biotas that contain bioactive compounds that have the potential as antibacterial ingredients. The purpose of this study was to perform antibacterial testing on fractions of the extract of the sea cucumber H. atra meat and to conduct a zoochemical analysis to determine the content of bioactive compounds that can inhibit bacterial growth. Antibacterial testing using disc diffusion method against Staphylococcus aureus and Escherichia coli bacteria. The results showed that the methanol extract of sea cucumber flesh could inhibit the growth of both types of test bacteria. In antibacterial testing for S. aureus, the ethyl acetate fraction was 11.8 mm, the n-hexane fraction was 7 mm, and the methanol fraction was 8.6 mm, while for E. coli the ethyl acetate fraction was 10.88 mm, the n-hexane fraction was 7 mm, and 8.6 mm methanol fraction. The compounds contained in the ethyl acetate fraction of sea cucumber H. atra flesh extract are alkaloids, flavonoids, steroids, and saponins which are compounds that can inhibit bacterial growth with their respective working mechanisms.Keywords: Disc diffusion, Extraction, Fractionation, Sea cucumber (H.atra), Zoo-chemical, AbstrakTeripang merupakan salah satu biota laut yang memiliki kandungan senyawa bioaktif yang berpotensi sebagai bahan antibakteri. Tujuan penelitian ini melakukan pengujian antibakteri pada fraksi-fraksi dari ekstrak daging teripang H. atra dan melakukan analisis zookimia untuk mengetahui kandungan senyawa bioaktif yang dapat menghambat pertumbuhan bakteri. Pengujian antibakteri menggunakan metode difusi cakram terhadap bakteri Staphylococcus aureus dan Escherichia coli. Hasil penelitian menunjukkan bahwa ekstrak metanol daging teripang memiliki dapat menghambat pertumbuhan kedua jenis bakteri uji. Pengujian antibakteri terhadap S. aureus fraksi etil asetat sebesar 11,8 mm, fraksi n-heksan 7 mm, dan fraksi metanol 8,6 mm sedangkan untuk bakteri E. coli fraksi etil asetat sebesar 10,88 mm, fraksi n heksana 7 mm, dan fraksi metanol 8,6 mm. Kandungan senyawa yang yang terkandung dalam fraksi etil asetat ekstrak daging teripang H. atra yaitu senyawa alkaloid, flavonoid, steroid, dan saponin yang merupakan senyawa yang dapat menghambat pertumbuhan bakteri dengan mekanisme kerja masing-masing.Kata Kunci  : Difusi cakram, Ekstraksi, Fraksinasi , Teripang (H. atra), Zoo-kimia,
Analysis Of Weather Conditions and Hydrodynamics in Taman Nasional Bunaken Ben Arther Molle; Joshian Nicolas William Schaduw; Deiske Adeliene Sumilat; Ari Berty Rondonuwu; Alfret Luasunaung; Veibe Warouw
Jurnal Ilmiah PLATAX Vol. 10 No. 2 (2022): ISSUE JULY-DECEMBER 2022
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.v10i2.43161

Abstract

Taman Nasional Bunaken (TNB) is located in North Sulawesi Province, Republic of Indonesia. TNB offers an exploration experience of marine tourism such as snorkeling and diving. This study was conducted to determine the statistics and correlation of weather parameters on hydrodynamics in the waters of the small island of TNB. This information can be used by marine tourism users. The weather data studied are wind direction and speed. The hydrodynamic conditions studied were wave height and ocean currents. Respondent surveys were also conducted on tourist users at the research sites. The wind direction for the period January - April is dominant from the Northeast, May - September is dominant from the East to the South, October tends to be evenly distributed from the East to the West, and November - December is dominant from the West. Significant wind speeds that occur in January, February, and March range from 18 – 36 knots. The average wave height shows a significant value in January – March, tends to be lower in April – June, fluctuates in July – November, and increases again in December. The maximum current velocity at a sea depth of 16.5 m is stronger than the maximum speed at a depth of 0.5 m and 8.0 m in January – April, and May August. The correlation value of wind speed and sea wave height is significant at 0.96 and the correlation between wind speed and the current speed is 0.74. These results indicate that there is a strong -very strong relationship between parameters. The survey results show that wind speed, wave height, and ocean currents have the same pattern. January, February, November, and December are the months of extreme or bad weather and hydrodynamic conditions.Keywords: Taman Nasional Bunaken, Weather, Hydrodynamics, CorrelationAbstrakTaman Nasional Bunaken (TNB) terletak di Provinsi Sulawesi Utara, Negara Republik Indonesia. TNB  menawarkan pengalaman eksplorasi suasana wisata bahari seperti snorkling dan diving. Penelitian ini dilakukan untuk mengetahui statistik dan korelasi parameter cuaca terhadap hidrodinamika di perairan pulau kecil TNB. Informasi tersebut dapat digunakan oleh pengguna wisata bahari. Data cuaca yang diteliti adalah arah dan kecepatan angin. Kondisi hidrodinamika yang diteliti ialah tinggi gelombang dan arus laut. Survei responden juga dilakukan kepada pengguna wisata di lokasi penelitian. Arah angin periode Bulan Januari - April dominan dari arah Timur Laut, Bulan Mei – September dominan dari arah Timur hingga Selatan, Bulan Oktober cenderung merata dari arah Timur hingga Barat dan Bulan November – Desember dominan dari arah Barat. Kecepatan angin signifikan terjadi pada bulan Januari, Februari, dan Maret berkisar antara 18 – 36 Knot. Rata-rata tinggi gelombang menunjukan nilai signifikan pada bulan Januari – Maret, cenderung merendah pada bulan April – Juni, berfluktuasi pada bulan Juli – November dan meningkat kembali di bulan Desember. Kecepatan maksimum arus laut di kedalaman 16.5 m lebih kuat jika dibandingkan kecepatan maksimum di kedalaman 0.5 m dan 8.0 m pada bulan Januari – April serta bulan Mei – Agustus. Nilai korelasi kecepatan angin dan tinggi gelombang laut signifikan sebesar 0.96 serta korelasi kecepatan angin dan kecepatan arus sebesar 0.74. Hasil tersebut menunjukkan antar parameter memiliki hubungan yang kuat - sangat kuat. Hasil survei menunjukan kecepatan angin, tinggi gelombang dan arus laut mempunyai pola yang sama. Bulan Januari, Februari, November, dan Desember merupakan bulan-bulan terjadinya kondisi cuaca serta hidrodinamika ekstrem atau buruk.Kata kunci: Taman Nasional Bunaken, Cuaca, Hidrodinamika, Korelasi
Antibacterial potential of marine fungus Aspergillus nomius isolated from green algae Bornetella sp Deiske Adeliene Sumilat; Rosita A. J. Lintang
Jurnal Ilmiah PLATAX Vol. 9 No. 1 (2021): ISSUE JANUARY - JUNE 2021
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.9.1.2021.33781

Abstract

Isolation of marine fungi symbiont of green algae Bornetella sp as a producer of antibacterial compounds has been carried out. This study aims to obtain symbiont fungi from green algae Bornetella sp which produces antibacterial compounds. The symbiont fungus was isolated using the direct planting method. Screening for the antibacterial activity of pure symbiont fungi isolates against Escheriscia coli and Staphylococcus aureus, and was carried out by the agar diffusion method. The isolation results obtained 4 isolates, but the one with the most potential to inhibit the growth of the tested bacteria was MFALM2. Molecular characterization showed that the MFALM2 isolate was identified as Aspergillus nomius with a 100% closeness level. Keywords: isolation; marine fungi; Aspergillus nomius; green alga; Bornetella sp. AbstrakIsolasi jamur laut yang bersimbion dengan alga hijau Bornetella sp sebagai penghasil senyawa antibakteri telah dilakukan. Penelitian ini bertujuan untuk memperoleh jamur simbion dari alga hijau Bornetella sp yang menghasilkan senyawa antibakteri. Isolasi jamur simbion dilakukan dengan metode direct planting. Skrining aktivitas antibakteri isolat murni jamur simbion terhadap bakteri uji Escheriscia coli dan Staphylococcus aureus dilakukan dengan metode difusi agar.  Hasil isolasi diperoleh 4 isolat, namun yang paling berpotensi menghambat pertumbuhan bakteri uji adalah MFALM2. Karakterisasi molekuler menunjukkan bahwa isolat MFALM2 teridentifikasi sebagai Aspergillus nomius dengan tingkat keeratan sebesar 100%.Kata kunci: isolasi; jamur lau; Aspergillus nomius; alga hijau; Bornetella sp.
Study of Planned Maintenance System Senior Admiral John Lie’s Training Ship on Shipping Safety Azhar Ariansyah Ansar; Alfret Luasunaung; Deiske Adeliene Sumilat; Revols Dolfi Ch Pamikiran; Frangky Erens Kaparang; Hens Onibala
Jurnal Ilmiah PLATAX Vol. 11 No. 2 (2023): ISSUE JULY-DECEMBER 2023
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.v11i2.48973

Abstract

The implementation of the ship maintenance management system on Rear Admiral Jhon Lie's training ship did not run optimally, which impacted the less-than-optimal condition of various training ship equipment because the crew did not carry out the ship maintenance system according to the procedure, resulting in the incident that the ship's anchor winch operation did not function optimally when the ship was anchored in Belang Port, Southeast Minahasa. This study aims to identify and analyze the positive and significant effect of the planned maintenance system on shipping safety. The primary data collection methods used in this study were questionnaires and interviews. In this study, data processing and presentation were in the form of diagrams and path analysis with structural equation modeling (SEM-PLS) using SmartPLS. From the results of the data analysis, the R-square value of shipping safety (Y) is 0.307, which means that the planned maintenance system (X1) and ship maintenance (X2) can affect shipping safety (Y) by 30.7%. Ship maintenance (X2) has a significant effect on sailing safety (Y), with P-values = 0.033 0.05 (hypothesis accepted). The planned maintenance system (X1) has a significant effect on sailing safety (Y), with a P-value of 0.037 0.05 (hypothesis accepted). As a result of the analysis, it was found that the planned maintenance system has a significant effect on shipping safety.
Sponge Diversity in Kinamang Beach Area Malalayang District Manado City Putra Musak; Deiske Adeliene Sumilat; Joshian Nicolas William Schaduw; Antonius Petrus Rumengan; Esther Dellayani Angkouw; Suzanne Lydia Undap
Jurnal Ilmiah PLATAX Vol. 11 No. 2 (2023): ISSUE JULY-DECEMBER 2023
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.v11i2.48599

Abstract

Spons are primitive living settled animals that are filter feeders. These animals are commonly found in tropical and subtropical waters, ranging from the intertidal zone to the subtidal. The aim of this study is to know the diversity of species and the content of bioactive sponges. Sponge data collection: ecological index, species composition, and density of sponge as well as knowledge of the study of bioactive sponge using the library study of the research carried out and using the scientific article of the last 10 years, from 2013 to 2023. The study used the transect belt method that has been modified. From the use of the method, 17 Families were obtained, 48 individuals at a depth of 7 m and 118 individuals at 14 m. Based on the results of this study showed that the index of diversity obtained from the analysis results belonged to the average in 7 m and 14 m. Distribution patterns obtained from data analysis results obtain the distribution pattern at a depth of 7 m grouping 5 families, uniform 5 families, and random 1 family. At a depth of 14 m, the most spread pattern is the uniform spread of 10 families, and the spread model groups 4 families. The index of diversity obtained from the results of data analysis is high (stable). The composition of the species obtained from the results of the analysis showed the highest species of sponges at a depth of 7 m was 33.33%, the highest species at a deep of 14 m is 33.90%, and the composition type of the lowest sponge depth 7 m is 0%, the lower species on a deep 14 m was 11.86%. The highest value of spongy density at a depth of 7 meters is 2.44 ind/m² and the lowest is 0.15 ind/m², whereas, at depths of 14 meters, the highest values are 3.16 ind/m² and lower is 0.08 ind/m² and a library study of the bioactive content received 27 articles related to the family obtaining at the research site and in teluk manado. Keywords: Diversity, Sponge, Kinamang Beach, Bioactive Content Abstrak  Spons merupakan hewan primitif yang hidup menetap yang bersifat filter feeder (menyaring). Hewan ini sangat umum dijumpai di perairan tropis dan sub tropis, sebarannya mulai dari zona intertidal hingga zona subtidal. Tujuan dari penelitian ini yaitu mengetahui keanekaragaman jenis dan kandungan bioaktif spons. Pengambilan data spons : indeks ekologi, komposisi jenis dan kepadatan spons serta mengetahui kajian mengenai bioaktif spons dengan menggunakan studi pustaka dari penelitian yang telah dilakukan dan menggunakan artikel ilmiah 10 tahun terakhir tahun 2013 sampai 2023. Penelitian ini menggunakan metode belt transek yang telah dimodifikasi. Dari pengunaan metode tersebut diperoleh 17 Famili, 48 individu pada kedalaman 7 m dan 118 individu pada kedalaman 14 m. Berdasarkan hasil dari penelitian ini menunjukkan bahwa indeks keanekaragaman yang diperoleh dari hasil analisis tergolong sedang pada kedalam 7 m dan 14 m. Pola Sebaran yang diperoleh dari hasil analisis data didapatkan pola sebaran pada kedalaman 7 m pola sebaran mengelompok 5 famili, pola sebaran seragam 5 famili,dan pola sebaran acak 1 famili. Sedangkan pada kedalaman 14 m pola sebaran terbanyak yaitu, pola sebaran seragam 10 famili, dan pola sebaran mengelompok 4 famili. Indeks keseragaman yang diperoleh dari hasil analisis data yaitu tergolong tinggi (stabil). Komposisi jenis yang diperoleh dari hasil analisis menunjukkan jenis spons tertinggi pada kedalaman 7 m yaitu 33,33 %, jenis spons tertinggi pada kedalaman 14 m yaitu 33,90 %, dan komposisi jenis spons terendah kedalaman 7 m yaitu 0 %,jenis spons terendah pada kedalaman 14 m yaitu 11,86 %. Nilai kepadatan spons tertinggi pada kedalaman 7 m yaitu 2,44 ind/m² dan terendah yaitu 0,15 ind/m², sedangkan pada kedalaman 14 meter dengan nilai tertinggi yaitu 3,16 ind/m² dan terendah yaitu 0,08 ind/m² dan studi pustaka mengenai kandungan bioaktif yang didapatkan 27 artikel yang berkaitan dengan famili yang didapatkan di lokasi penelitian dan di teluk manado. Kata Kunci: Keanekaragaman, Spons, Pantai Kinamang, Kandungan Bioaktif
Co-Authors Alfret Luasunaung Antonius Petrus Rumengan Ari Berty Rondonuwu Azhar Ariansyah Ansar Ben Arther Molle Esther Dellayani Angkouw Fitje Losung Frangky Erens Kaparang Hens Onibala Joshian Nicolas William Schaduw Joshian Nicolas William Schaduw Lawrence J. L Lumingas Putra Musak Revols Dolfi Ch Pamikiran Rosita A. J. Lintang Rosita Anggreiny Lintang Suzanne Lydia Undap Theresia Tessa Mewengkang Veibe Warouw